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Hypoxia Enhances Mesenchymal Characteristics Maintenance of Buffalo Bone Marrow-Derived Mesenchymal Stem Cells.
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- Author(s): Zhang J;Zhang J; Lei C; Lei C; Deng Y; Deng Y; Ahmed JZ; Ahmed JZ; Shi D; Shi D; Lu F; Lu F
- Source:
Cellular reprogramming [Cell Reprogram] 2020 Jun; Vol. 22 (3), pp. 167-177. Date of Electronic Publication: 2020 May 21.- Publication Type:
Journal Article; Research Support, Non-U.S. Gov't- Language:
English - Source:
- Additional Information
- Source: Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 101528176 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2152-4998 (Electronic) Linking ISSN: 21524971 NLM ISO Abbreviation: Cell Reprogram Subsets: MEDLINE
- Publication Information: Original Publication: New Rochelle, NY : Mary Ann Liebert, Inc.
- Subject Terms: Bone Marrow Cells/*metabolism ; Hypoxia/*metabolism ; Hypoxia-Inducible Factor 1, alpha Subunit/*metabolism ; Mesenchymal Stem Cells/*metabolism ; Twist-Related Protein 1/*metabolism; Animals ; Bone Marrow Cells/cytology ; Buffaloes ; Cell Differentiation/physiology ; Cell Hypoxia/physiology ; Cells, Cultured ; Mesenchymal Stem Cells/cytology ; Signal Transduction ; Stress, Mechanical
- Abstract: Bone marrow-derived mesenchymal stem cells (BMSCs) from livestock are valuable resources for veterinary therapeutics and animal reproduction. Previous studies have shown that hypoxic conditions were beneficial in maintaining the mesenchymal feature of BMSCs. However, the effects of hypoxia on buffalo BMSCs (bBMSCs) remain unclear. In this study, the effects of hypoxic conditions on cell morphology, migration, polarity, and karyotype of bBMSCs were examined. The results showed that hypoxia (5% oxygen) enhanced colony formation and stress fiber synthesis of bBMSCs. Under the hypoxic culture conditions, the migration capacity and normal karyotype rate of bBMSCs were significantly improved ( p < 0.05), which resulted in weakened cell polarity and enhanced karyotype stability in bBMSCs. In addition, it was significantly ( p < 0.05) upregulated in the expression levels of HIF-TWIST signaling pathway axis-related genes ( Hif-1 , Hif-2 , Twist , Snail , Slug , Fn1 , N-cadherin , Collal ). The HIF-TWIST axis of bBMSCs was also activated in hypoxia. Finally, it was more effective and easier to maintain the mesenchymal feature of bBMSCs in hypoxic conditions. These findings not only provide theoretical guidance to elucidate the detailed regulation mechanism of hypoxia on mesenchymal nature maintenance of bBMSCs, but also provide positive support to further establish the stable in vitro culture system of bBMSCs.
- Contributed Indexing: Keywords: buffalo bone marrow-derived mesenchymal stem cells; hypoxia; maintenance; mesenchymal characteristics
- Accession Number: 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
0 (Twist-Related Protein 1) - Publication Date: Date Created: 20200527 Date Completed: 20210614 Latest Revision: 20210614
- Publication Date: 20240105
- Accession Number: 10.1089/cell.2019.0097
- Accession Number: 32453601
- Source:
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