Protecting HaCaT cells from ionizing radiation using persimmon tannin- Aloe gel composite.

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  • Additional Information
    • Source:
      Publisher: Taylor & Francis Country of Publication: England NLM ID: 9812552 Publication Model: Print Cited Medium: Internet ISSN: 1744-5116 (Electronic) Linking ISSN: 13880209 NLM ISO Abbreviation: Pharm Biol Subsets: MEDLINE
    • Publication Information:
      Publication: [London] : Taylor & Francis
      Original Publication: Lisse, the Netherlands : Swets & Zeitlinger, c1998-
    • Subject Terms:
    • Abstract:
      Context: Persimmon tannin (extract of Diospyros kaki L.f [Ebenaceae]) and Aloe gel ( extract of Aloe vera (L.) Burm.f. [Asphodelaceae]) are known as anti-radiation agents. However, radiation resistance of the persimmon tannin- Aloe gel composite remains inconclusive. Objective: To investigate the capacity of the persimmon tannin- Aloe gel composite to protect against ionising radiation at the cellular level. Materials and methods: HaCaT (human epidermal keratinocytes) cells were pre-treated with PT-A-1 (the mass ratio of persimmon tannin and Aloe gel was 2:1) or the single component (persimmon tannin or Aloe gel) at various concentrations (0, 50, 100, 200, 400, 800 μg/mL. Control group: medium with no HaCaT cells), and then radiated with X-rays (radiation dose: 4, 8, 12, 16, and 20 Gy). Cell viability, cell apoptosis, and radiation-induced intracellular reactive oxygen species (ROS) generation were analysed by CCK-8, Hoechst 33258 staining/flow cytometry, and 2',7'-dichlorfluorescein diacetate (DCFH-DA) assay, respectively, for 12 or 24 h incubation after radiation. Results: The optimal radiation dose and post-radiation incubation period were determined to be 8 Gy and 12 h. CCK-8 activity detection showed that the cell activity was 77.85% ( p  < 0.05, IC 50 = 55.67 μg/mL). The apoptotic rate was the lowest (4.32%) at 200 μg/mL of PT-A-1 towards HaCaT cells. ROS production was the most effectively suppressed by 200 μg/mL PT-A-1 towards HaCaT cells. Discussion and conclusions: The persimmon tannin- Aloe gel composite has good radioprotective effect, and which will facilitate its clinic application as a potential natural anti-radiation agent in future.
    • References:
      Biomed Pharmacother. 2019 Mar;111:151-161. (PMID: 30579254)
      J Environ Radioact. 2018 Dec;192:32-47. (PMID: 29883875)
      J Environ Radioact. 2016 Oct;162-163:182-188. (PMID: 27267156)
      J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Jul 15;1058:57-67. (PMID: 28535423)
      Environ Technol. 2019 Jan;40(1):112-124. (PMID: 28911271)
      Radiother Oncol. 2011 Nov;101(2):329-36. (PMID: 22079530)
      Brain Res Bull. 2012 Jan 4;87(1):74-9. (PMID: 22020131)
      Int J Biol Macromol. 2015;77:336-43. (PMID: 25841373)
      J Thorac Oncol. 2015 Dec;10(12):1703-12. (PMID: 26709479)
      Mater Sci Eng C Mater Biol Appl. 2017 Oct 1;79:227-236. (PMID: 28629012)
      Mutat Res. 2018 Sep;811:1-15. (PMID: 30014950)
      J Ayurveda Integr Med. 2016 Mar;7(1):62-8. (PMID: 27240731)
      Complement Ther Clin Pract. 2019 May;35:126-130. (PMID: 31003648)
      J Environ Manage. 2018 Jul 1;217:305-314. (PMID: 29614479)
      Cell J. 2016 Spring;18(1):46-51. (PMID: 27054118)
      Cell Stress Chaperones. 2015 Mar;20(2):289-95. (PMID: 25181965)
      Pharm Biol. 2015 Apr;53(4):540-7. (PMID: 25356658)
      Fitoterapia. 2017 Jul;120:41-51. (PMID: 28552595)
      Carbohydr Polym. 2019 Mar 1;207:460-470. (PMID: 30600029)
      Acta Crystallogr D Biol Crystallogr. 2000 Mar;56(Pt 3):328-41. (PMID: 10713520)
    • Contributed Indexing:
      Keywords: Human epidermal keratinocytes cells; Radiation resistance; X-rays
    • Accession Number:
      0 (Gels)
      0 (Plant Extracts)
      0 (Tannins)
    • Publication Date:
      Date Created: 20200602 Date Completed: 20210326 Latest Revision: 20211205
    • Publication Date:
      20240104
    • Accession Number:
      PMC8641672
    • Accession Number:
      10.1080/13880209.2020.1767158
    • Accession Number:
      32476533