Regulation of epithelial integrity and organ growth by Tctp and Coracle in Drosophila.

Publisher: Public Library of Science Country of Publication: United States NLM ID: 101239074 Publication Model: eCollection Cited Medium: Internet ISSN: 1553-7404 (Electronic) Linking ISSN: 15537390 NLM ISO Abbreviation: PLoS Genet Subsets: MEDLINE

Original Publication: San Francisco, CA : Public Library of Science, c2005-

Drosophila Proteins/*metabolism ; Drosophila melanogaster/*physiology ; Epithelium/*metabolism ; Intracellular Signaling Peptides and Proteins/*metabolism ; Membrane Proteins/*metabolism ; Wings, Animal/*growth & development; Animals ; Animals, Genetically Modified ; Cell Proliferation/genetics ; Drosophila Proteins/genetics ; Embryo, Nonmammalian ; Female ; Gene Expression Regulation, Developmental ; Gene Knockdown Techniques ; Imaginal Discs/growth & development ; Intercellular Junctions/metabolism ; Intracellular Signaling Peptides and Proteins/genetics ; Male ; Membrane Proteins/genetics ; Morphogenesis/genetics ; Synthetic Lethal Mutations ; Wings, Animal/metabolism

Regulation of cell junctions is crucial for the integrity of epithelial tissues and organs. Cell junctions also play roles in controlling cell proliferation for organ growth. Translationally controlled tumor protein (TCTP) is a conserved protein involved in growth control, but its role in cell junctions is unknown. Here we show that Drosophila Tctp directly interacts with the septate junction protein Coracle (Cora) to regulate epithelial integrity and organ growth. Tctp localizes together with Cora in the epidermis of the embryo. Loss of Cora reduces the level of Tctp in the epidermis but not vice versa. cora/+ or Tctp/+ single heterozygotes develop normally to adulthood. However, double heterozygotes for cora and Tctp mutations show severe disruption of epithelia causing synthetic lethality in the embryo. Double knockdown of Cora and Tctp in eye imaginal disc synergistically leads to disruption of the eye disc, resulting in a severe reduction or loss of eye and head. Conversely, double knockdown of Cora and Tctp in wing disc causes overgrowth as well as cell death. Inhibition of cell death under this condition causes hyperplastic growth of the wing disc. Tctp also shows direct and functional interaction with Cora-associated factors like Yurt and Na+/K+-ATPase. This study suggests that proper levels of Tctp and Cora are essential for the maintenance of the Cora complex and the integrity of epithelia. Our data also provide evidence that both Cora and Tctp are required to suppress overgrowth in developing wing.
Competing Interests: The authors have declared that no competing interests exist.

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0 (Drosophila Proteins)
0 (Intracellular Signaling Peptides and Proteins)
0 (Membrane Proteins)
0 (TCTP protein, Drosophila)
0 (cora protein, Drosophila)
0 (yrt protein, Drosophila)

Date Created: 20200620 Date Completed: 20200831 Latest Revision: 20200831

20240105

PMC7329144

10.1371/journal.pgen.1008885

32559217