Differentiation-dependent susceptibility of human muscle cells to Zika virus infection.

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    • Source:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101291488 Publication Model: eCollection Cited Medium: Internet ISSN: 1935-2735 (Electronic) Linking ISSN: 19352727 NLM ISO Abbreviation: PLoS Negl Trop Dis Subsets: MEDLINE
    • Publication Information:
      Original Publication: San Francisco, CA : Public Library of Science
    • Subject Terms:
    • Abstract:
      Muscle cells are potential targets of many arboviruses, such as Ross River, Dengue, Sindbis, and chikungunya viruses, that may be involved in the physiopathological course of the infection. During the recent outbreak of Zika virus (ZIKV), myalgia was one of the most frequently reported symptoms. We investigated the susceptibility of human muscle cells to ZIKV infection. Using an in vitro model of human primary myoblasts that can be differentiated into myotubes, we found that myoblasts can be productively infected by ZIKV. In contrast, myotubes were shown to be resistant to ZIKV infection, suggesting a differentiation-dependent susceptibility. Infection was accompanied by a caspase-independent cytopathic effect, associated with paraptosis-like cytoplasmic vacuolization. Proteomic profiling was performed 24h and 48h post-infection in cells infected with two different isolates. Proteome changes indicate that ZIKV infection induces an upregulation of proteins involved in the activation of the Interferon type I pathway, and a downregulation of protein synthesis. This work constitutes the first observation of primary human muscle cells susceptibility to ZIKV infection, and differentiation-dependent restriction of infection from myoblasts to myotubes. Since myoblasts constitute the reservoir of stem cells involved in reparation/regeneration in muscle tissue, the infection of muscle cells and the viral-induced alterations observed here could have consequences in ZIKV infection pathogenesis.
      Competing Interests: The authors have declared that no competing interests exist.
    • References:
      J Neurovirol. 2018 Feb;24(1):52-61. (PMID: 29063515)
      Pediatr Infect Dis J. 2010 Mar;29(3):238-42. (PMID: 20032806)
      J Neurol Sci. 2011 Jan 15;300(1-2):14-8. (PMID: 21081241)
      Cell Death Differ. 2004 Oct;11(10):1066-75. (PMID: 15195070)
      Nucleic Acids Res. 2017 Jan 4;45(D1):D362-D368. (PMID: 27924014)
      EMBO J. 2017 Jun 14;36(12):1653-1668. (PMID: 28473450)
      Euro Surveill. 2014 Mar 06;19(9):. (PMID: 24626205)
      Proc Natl Acad Sci U S A. 2000 Dec 19;97(26):14376-81. (PMID: 11121041)
      Trends Microbiol. 2015 Jan;23(1):35-43. (PMID: 25449049)
      Ann Intern Med. 2017 Jan 17;166(2):99-108. (PMID: 27893080)
      Case Rep Rheumatol. 2016;2016:5395249. (PMID: 27119037)
      Trans R Soc Trop Med Hyg. 1952 Sep;46(5):509-20. (PMID: 12995440)
      J Clin Diagn Res. 2016 Dec;10(12):OD05-OD06. (PMID: 28208912)
      MMWR Morb Mortal Wkly Rep. 2016 Sep 30;65(38):1032-8. (PMID: 27684886)
      N Engl J Med. 2009 Jun 11;360(24):2536-43. (PMID: 19516034)
      Vet Res. 2016 Jan 08;47:9. (PMID: 26743565)
      Nat Protoc. 2009;4(1):44-57. (PMID: 19131956)
      Infect Genet Evol. 2017 Apr;49:134-137. (PMID: 28095299)
      Cell Death Dis. 2017 Oct 12;8(10):e3106. (PMID: 29022904)
      PLoS Pathog. 2008 Feb 8;4(2):e29. (PMID: 18282093)
      Int J Mol Sci. 2016 Dec 09;17(12):. (PMID: 27941671)
      PLoS Pathog. 2017 Mar 9;13(3):e1006219. (PMID: 28278237)
      PLoS One. 2007 Jun 13;2(6):e527. (PMID: 17565380)
      Emerg Infect Dis. 2014 Jun;20(6):1085-6. (PMID: 24856001)
      Nature. 2008 Sep 11;455(7210):242-5. (PMID: 18690214)
      Euro Surveill. 2016 Jul 14;21(28):. (PMID: 27447300)
      J Infect Dis. 2012 Aug 1;206(3):407-14. (PMID: 22615321)
      Nucleic Acids Res. 2015 Apr 20;43(7):e47. (PMID: 25605792)
      Aging Cell. 2007 Aug;6(4):515-23. (PMID: 17559502)
      Bioinformatics. 2006 Aug 15;22(16):1979-87. (PMID: 16777905)
      Hum Gene Ther. 1997 Aug 10;8(12):1429-38. (PMID: 9287143)
      J Biol Chem. 2011 Feb 18;286(7):5151-6. (PMID: 21149444)
      J Virol. 2015 Sep;89(17):8880-96. (PMID: 26085147)
      Nat Microbiol. 2018 Mar;3(3):302-309. (PMID: 29379210)
      Lancet. 2016 May 21;387(10033):2125-2132. (PMID: 26993883)
      Am J Trop Med Hyg. 2016 Jun 1;94(6):1362-1369. (PMID: 27022155)
      Lancet. 2016 Apr 9;387(10027):1531-1539. (PMID: 26948433)
      Bioinformatics. 2017 Jan 1;33(1):135-136. (PMID: 27605098)
      PLoS One. 2013 Nov 05;8(11):e79628. (PMID: 24223983)
      Cell Stem Cell. 2016 May 5;18(5):587-90. (PMID: 26952870)
      Virol J. 2011 Sep 08;8:432. (PMID: 21902836)
      Lancet. 2016 Aug 27;388(10047):868. (PMID: 27597462)
      Proteomics. 2016 Jan;16(1):29-32. (PMID: 26572953)
      Virology. 2016 Jun;493:217-26. (PMID: 27060565)
      Cell Host Microbe. 2016 Aug 10;20(2):155-66. (PMID: 27443522)
      Cell Stem Cell. 2016 Nov 3;19(5):672. (PMID: 27814481)
      Nat Cell Biol. 2013 May;15(5):472-80. (PMID: 23604317)
      Southeast Asian J Trop Med Public Health. 2015;46 Suppl 1:149-52. (PMID: 26506741)
      J Proteome Res. 2015 Aug 7;14(8):3348-61. (PMID: 26074025)
      Sci Rep. 2017 Jan 23;7:40780. (PMID: 28112162)
      Infection. 2012 Apr;40(2):125-30. (PMID: 22005935)
      Cell Stem Cell. 2016 May 5;18(5):591-6. (PMID: 27038591)
      J Virol. 2006 Jan;80(2):737-49. (PMID: 16378976)
      PLoS Negl Trop Dis. 2016 Apr 19;10(4):e0004682. (PMID: 27093158)
      Front Neurol. 2012 Mar 21;3:37. (PMID: 22461779)
      Sci Rep. 2016 Feb 19;6:21406. (PMID: 26892458)
      Mol Cell Proteomics. 2014 Sep;13(9):2513-26. (PMID: 24942700)
      Cell Res. 2016 Jul;26(7):753-4. (PMID: 27283801)
      Antiviral Res. 2018 Dec;160:38-47. (PMID: 30326204)
      Antiviral Res. 2010 Sep;87(3):281-94. (PMID: 20452379)
      PLoS Negl Trop Dis. 2019 May 3;13(5):e0007375. (PMID: 31050676)
      J Immunol. 2000 May 15;164(10):5459-65. (PMID: 10799913)
      J Gen Virol. 2008 Jul;89(Pt 7):1605-1615. (PMID: 18559930)
      Cell Rep. 2017 Jan 10;18(2):324-333. (PMID: 28076778)
      Vector Borne Zoonotic Dis. 2005 Fall;5(3):252-7. (PMID: 16187894)
      J Gen Virol. 2017 Aug;98(8):2061-2068. (PMID: 28786784)
      Emerg Microbes Infect. 2016 Aug 24;5:e93. (PMID: 27553173)
      Brain Res Bull. 2017 Jan;128:40-47. (PMID: 27871950)
      MMWR Morb Mortal Wkly Rep. 2016 May 06;65(17):451-5. (PMID: 27149205)
    • Accession Number:
      0 (Interferon Type I)
      0 (Proteins)
    • Publication Date:
      Date Created: 20200821 Date Completed: 20201007 Latest Revision: 20201007
    • Publication Date:
      20240105
    • Accession Number:
      PMC7508361
    • Accession Number:
      10.1371/journal.pntd.0008282
    • Accession Number:
      32817655