Selection of GmSWEET39 for oil and protein improvement in soybean.

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  • Additional Information
    • Source:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101239074 Publication Model: eCollection Cited Medium: Internet ISSN: 1553-7404 (Electronic) Linking ISSN: 15537390 NLM ISO Abbreviation: PLoS Genet Subsets: MEDLINE
    • Publication Information:
      Original Publication: San Francisco, CA : Public Library of Science, c2005-
    • Subject Terms:
      Plant Breeding*; Monosaccharide Transport Proteins/*genetics ; Plant Proteins/*genetics ; Glycine max/*genetics; Chromosome Mapping ; Genome, Plant/genetics ; Genome-Wide Association Study ; Haplotypes ; Monosaccharide Transport Proteins/metabolism ; North America ; Plant Oils/metabolism ; Plant Proteins/metabolism ; Plant Proteins, Dietary/biosynthesis ; Polymorphism, Single Nucleotide ; Quantitative Trait Loci ; Seeds/metabolism ; Glycine max/metabolism
    • Abstract:
      Soybean [Glycine max (L.) Merr.] was domesticated from wild soybean (G. soja Sieb. and Zucc.) and has been further improved as a dual-use seed crop to provide highly valuable oil and protein for food, feed, and industrial applications. However, the underlying genetic and molecular basis remains less understood. Having combined high-confidence bi-parental linkage mapping with high-resolution association analysis based on 631 whole sequenced genomes, we mapped major soybean protein and oil QTLs on chromosome15 to a sugar transporter gene (GmSWEET39). A two-nucleotide CC deletion truncating C-terminus of GmSWEET39 was strongly associated with high seed oil and low seed protein, suggesting its pleiotropic effect on protein and oil content. GmSWEET39 was predominantly expressed in parenchyma and integument of the seed coat, and likely regulates oil and protein accumulation by affecting sugar delivery from maternal seed coat to the filial embryo. We demonstrated that GmSWEET39 has a dual function for both oil and protein improvement and undergoes two different paths of artificial selection. A CC deletion (CC-) haplotype H1 has been intensively selected during domestication and extensively used in soybean improvement worldwide. H1 is fixed in North American soybean cultivars. The protein-favored (CC+) haplotype H3 still undergoes ongoing selection, reflecting its sustainable role for soybean protein improvement. The comprehensive knowledge on the molecular basis underlying the major QTL and GmSWEET39 haplotypes associated with soybean improvement would be valuable to design new strategies for soybean seed quality improvement using molecular breeding and biotechnological approaches.
      Competing Interests: The authors have declared that no competing interests exist.
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    • Accession Number:
      0 (Monosaccharide Transport Proteins)
      0 (Plant Oils)
      0 (Plant Proteins)
      0 (Plant Proteins, Dietary)
    • Publication Date:
      Date Created: 20201111 Date Completed: 20210106 Latest Revision: 20240330
    • Publication Date:
      20240330
    • Accession Number:
      PMC7721174
    • Accession Number:
      10.1371/journal.pgen.1009114
    • Accession Number:
      33175845