SDF Factor-1α Promotes the Migration, Proliferation, and Osteogenic Differentiation of Mouse Bone Marrow Mesenchymal Stem Cells Through the Wnt/β-Catenin Pathway.

Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 101197107 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1557-8534 (Electronic) Linking ISSN: 15473287 NLM ISO Abbreviation: Stem Cells Dev Subsets: MEDLINE

Original Publication: Larchmont, NY : Mary Ann Liebert, Inc., c2004-

Cell Differentiation/*genetics ; Cell Movement/*genetics ; Cell Proliferation/*genetics ; Chemokine CXCL12/*genetics ; Mesenchymal Stem Cells/*metabolism ; Osteogenesis/*genetics ; Wnt Signaling Pathway/*genetics ; beta Catenin/*genetics; Alkaline Phosphatase/metabolism ; Animals ; Cell Differentiation/drug effects ; Cell Movement/drug effects ; Cell Proliferation/drug effects ; Cells, Cultured ; Chemokine CXCL12/metabolism ; Chemokine CXCL12/pharmacology ; Gene Expression Regulation/drug effects ; Lentivirus/genetics ; Mesenchymal Stem Cells/cytology ; Mice, Inbred C57BL ; Microscopy, Fluorescence ; Osteogenesis/drug effects ; RNA Interference ; Reverse Transcriptase Polymerase Chain Reaction ; Wnt Signaling Pathway/drug effects ; beta Catenin/metabolism ; Mice

Bone marrow mesenchymal stem cells (BMSCs) are thought to have great potential in the treatment of many diseases and may serve as a cell source for tissue engineering. These cells may be regulated by stromal cell-derived factor-1α (SDF-1α), which has been shown to promote the migration, proliferation, and osteogenic differentiation of BMSCs in inflammation-associated diseases. However, the specific mechanism underlying this process remains unclear. We herein transduced lentivirus carrying SDF-1α, empty vector, or siRNA-SDF-1α into mouse BMSCs and then performed transwell, CCK-8, cell cycle, alkaline phosphatase activity, and Alizarin Red staining experiments on the three groups of samples. Overexpression of SDF-1α promoted the migration, proliferation, and osteogenic differentiation of BMSCs, and SDF-1α upregulated the expression of Wnt pathway-related factors and downstream target genes as determined by western blot, real-time polymerase chain reaction, and immunofluorescence. The effect of low SDF-1α expression on BMSCs was significantly weakened. In addition, we transduced lentivirus carrying siRNA-Wnt3a into BMSCs and treated them with SDF-1 drugs. After inhibiting the Wnt pathway, SDF-1 significantly weakened the migration, proliferation, and osteogenic differentiation of BMSCs. From this, we concluded that high SDF-1 expression can promote the migration, proliferation, and osteogenic differentiation of BMSCs, at least in part by activating the Wnt pathway.

Keywords: Wnt/β-catenin; bone marrow mesenchymal stem cells; migration; osteogenic differentiation; proliferation; stromal cell-derived factor-1α

0 (Chemokine CXCL12)
0 (beta Catenin)
EC 3.1.3.1 (Alkaline Phosphatase)

Date Created: 20201125 Date Completed: 20211213 Latest Revision: 20240226

20240226

10.1089/scd.2020.0165

33234049