Prevalence and virulence gene profiles of Escherichia coli O157 from cattle slaughtered in Buea, Cameroon.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read Online Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
    • Publication Information:
      Original Publication: San Francisco, CA : Public Library of Science
    • Subject Terms:
      Escherichia coli Infections/*microbiology ; Escherichia coli O157/*genetics ; Virulence/*genetics ; Virulence Factors/*genetics; Animals ; Cameroon ; Cattle ; Escherichia coli Proteins/genetics ; Food Microbiology/methods ; Genetic Profile ; Meat/microbiology ; Polymerase Chain Reaction/methods ; Prevalence
    • Abstract:
      Background: Escherichia coli O157 is an emerging foodborne pathogen of great public health concern. It has been associated with bloody diarrhoea, haemorrhagic colitis and haemolytic uremic syndrome in humans. Most human infections have been traced to cattle and the consumption of contaminated cattle products. In order to understand the risk associated with the consumption of cattle products, this study sought to investigate the prevalence and identify virulence genes in E. coli O157 from cattle in Cameroon.
      Method: A total of 512 rectal samples were obtained and analysed using conventional bacteriological methods (enrichment on modified Tryptone Soy Broth and selective plating on Cefixime-Tellurite Sorbitol Mac-Conkey Agar) for the isolation of E. coli O157. Presumptive E. coli O157 isolates were confirmed serologically using E. COLIPROTM O157 latex agglutination test and molecularly using PCR targeting the rfb gene in the isolates. Characterisation of the confirmed E. coli O157 strains was done by amplification of stx1, stx2, eaeA and hlyA virulence genes using both singleplex and multiplex PCR.
      Results: E. coli O157 was detected in 56 (10.9%) of the 512 samples examined. The presence of the virulence genes stx2, eaeA and hylA was demonstrated in 96.4% (54/56) of the isolates and stx1 in 40 (71.4%) of the 54. The isolates exhibited three genetic profiles (I-III) with I (stx1, stx2, eaeA and hlyA) being the most prevalent (40/56; 71.4%) while two isolates had none of the virulence genes tested.
      Conclusion: A proportion of cattle slaughtered in abattoirs in Buea are infected with pathogenic E. coli O157 and could be a potential source of human infections. We recommend proper animal food processing measures and proper hygiene be prescribed and implemented to reduce the risk of beef contamination.
      Competing Interests: The authors have declared that no competing interests exist.
    • References:
      Clin Infect Dis. 2012 Jun;54 Suppl 5:S472-9. (PMID: 22572672)
      Appl Environ Microbiol. 2015 Feb;81(4):1397-1405. (PMID: 25527532)
      J Infect Dis. 1995 Nov;172(5):1397-401. (PMID: 7594687)
      Chin Med J (Engl). 2006 Feb 5;119(3):217-22. (PMID: 16537008)
      Nat Rev Microbiol. 2010 Feb;8(2):105-16. (PMID: 20023663)
      Microbiol Spectr. 2014 Aug;2(4):EHEC-0024-2013. (PMID: 25530917)
      J Clin Microbiol. 1991 May;29(5):985-9. (PMID: 2056066)
      Infect Immun. 2000 Jan;68(1):64-71. (PMID: 10603369)
      J Med Microbiol. 1993 Aug;39(2):155-8. (PMID: 8345511)
      Int J Microbiol. 2019 Nov 26;2019:2894328. (PMID: 31885595)
      Int J Food Microbiol. 2013 Aug 1;165(3):295-301. (PMID: 23803571)
      Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2014;31(6):1064-80. (PMID: 24684161)
      J Infect Dev Ctries. 2008 Oct 01;2(5):389-93. (PMID: 19745509)
      J Clin Microbiol. 1998 Feb;36(2):598-602. (PMID: 9466788)
      Appl Environ Microbiol. 2003 May;69(5):2444-7. (PMID: 12732509)
      Histochem Cell Biol. 2002 Feb;117(2):131-41. (PMID: 11935289)
      Jundishapur J Microbiol. 2015 Nov 21;8(11):e25317. (PMID: 26865935)
      Adv Appl Microbiol. 2014;86:145-97. (PMID: 24377855)
      Curr Issues Mol Biol. 2009;11(2):67-79. (PMID: 19351974)
      Infect Ecol Epidemiol. 2016 May 18;6:31055. (PMID: 27197617)
      Toxins (Basel). 2019 Aug 29;11(9):. (PMID: 31470552)
      Saudi J Biol Sci. 2015 Nov;22(6):725-9. (PMID: 26587000)
      Am J Trop Med Hyg. 2010 Aug;83(2 Suppl):58-64. (PMID: 20682907)
      Microbiol Spectr. 2014 Aug;2(4):EHEC-0027-2014. (PMID: 26104194)
      MMWR Morb Mortal Wkly Rep. 2018 Jun 15;67(23):659-662. (PMID: 29902164)
      BMC Microbiol. 2008 Mar 17;8:46. (PMID: 18366637)
      Epidemiol Infect. 2015 Jan;143(2):274-87. (PMID: 24731271)
      J Vet Med B Infect Dis Vet Public Health. 2006 Sep;53(7):299-305. (PMID: 16930272)
    • Accession Number:
      0 (Escherichia coli Proteins)
      0 (Virulence Factors)
    • Publication Date:
      Date Created: 20201215 Date Completed: 20210111 Latest Revision: 20210111
    • Publication Date:
      20240104
    • Accession Number:
      PMC7737970
    • Accession Number:
      10.1371/journal.pone.0235583
    • Accession Number:
      33320853