A mixture of quebracho and chestnut tannins drives butyrate-producing bacteria populations shift in the gut microbiota of weaned piglets.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read Online Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
    • Publication Information:
      Original Publication: San Francisco, CA : Public Library of Science
    • Subject Terms:
      Bacteria/*genetics ; Butyrates/*metabolism ; Nuts/*chemistry ; RNA, Ribosomal, 16S/*genetics ; Sequence Analysis, DNA/*methods ; Tannins/*administration & dosage; Animal Feed/analysis ; Animals ; Bacteria/classification ; Bacteria/drug effects ; Bacteria/isolation & purification ; DNA, Bacterial/genetics ; DNA, Ribosomal/genetics ; Feces/microbiology ; Female ; Gastrointestinal Microbiome/drug effects ; High-Throughput Nucleotide Sequencing ; Male ; Phylogeny ; Swine ; Tannins/chemistry ; Tannins/pharmacology ; Weaning
    • Abstract:
      Weaning is a critical period for piglets, in which unbalanced gut microbiota and/or pathogen colonisation can contribute to diseases that interfere with animal performance. Tannins are natural compounds that could be used as functional ingredients to improve gut health in pig farming thanks to their antibacterial, antioxidant, and antidiarrhoeal properties. In this study, a mixture of quebracho and chestnut tannins (1.25%) was evaluated for its efficacy in reducing the negative weaning effects on piglet growth. Microbiota composition was assessed by Illumina MiSeq 16S rRNA gene sequencing of DNA extracted from stools at the end of the trial. Sequence analysis revealed an increase in the genera Shuttleworthia, Pseudobutyrivibrio, Peptococcus, Anaerostipes, and Solobacterium in the tannin-supplemented group. Conversely, this dietary intervention reduced the abundance of the genera Syntrophococcus, Atopobium, Mitsuokella, Sharpea, and Prevotella. The populations of butyrate-producing bacteria were modulated by tannin, and higher butyrate concentrations in stools were detected in the tannin-fed pigs. Co-occurrence analysis revealed that the operational taxonomic units (OTUs) belonging to the families Veillonellaceae, Lachnospiraceae, and Coriobacteriaceae occupied the central part of the network in both the control and the tannin-fed animals. Instead, in the tannin group, the OTUs belonging to the families Acidaminococcaceae, Alcaligenaceae, and Spirochaetaceae characterised its network, whereas Family XIII Incertae Sedis occupied a more central position than in the control group. Conversely, the presence of Desulfovibrionaceae characterised the network of the control group, and this family was not present in the network of the tannin group. Moreover, the prediction of metabolic pathways revealed that the gut microbiome of the tannin group possessed an enhanced potential for carbohydrate transport and metabolism, as well as a lower abundance of pathways related to cell wall/membrane/envelope biogenesis and inorganic ion transport. In conclusion, the tested tannins seem to modulate the gut microbiota, favouring groups of butyrate-producing bacteria.
      Competing Interests: The authors have declared that no competing interests exist.
    • References:
      Food Funct. 2014 Sep;5(9):2298-308. (PMID: 25066634)
      Front Microbiol. 2016 Jun 28;7:979. (PMID: 27446020)
      Anim Sci J. 2020 Jan;91(1):e13418. (PMID: 32648357)
      Bioinformatics. 2014 Aug 1;30(15):2114-20. (PMID: 24695404)
      Molecules. 2015 Jul 13;20(7):12698-731. (PMID: 26184148)
      Appl Environ Microbiol. 2008 Oct;74(19):5986-90. (PMID: 18676709)
      Appl Environ Microbiol. 2017 Jul 17;83(15):. (PMID: 28526795)
      Appl Environ Microbiol. 2004 Oct;70(10):5810-7. (PMID: 15466518)
      Bioinformatics. 2009 Aug 15;25(16):2078-9. (PMID: 19505943)
      Appl Environ Microbiol. 2007 Jul;73(14):4484-90. (PMID: 17526792)
      Environ Microbiol. 2017 Mar;19(3):835-841. (PMID: 28028921)
      mBio. 2014 Apr 22;5(2):e00889. (PMID: 24757212)
      Microbiome. 2019 Jun 13;7(1):91. (PMID: 31196177)
      Front Microbiol. 2017 Aug 15;8:1555. (PMID: 28861066)
      Biomed Res Int. 2018 Feb 21;2018:1879168. (PMID: 29682522)
      Anim Nutr. 2017 Dec;3(4):322-330. (PMID: 29767133)
      Anim Sci J. 2019 May;90(5):680-689. (PMID: 30848035)
      Environ Microbiol Rep. 2015 Jun;7(3):554-69. (PMID: 25727666)
      Int J Syst Evol Microbiol. 2003 Jan;53(Pt 1):201-209. (PMID: 12656174)
      J Nutr. 1999 May;129(5):1002-9. (PMID: 10222392)
      Nutr Diabetes. 2014 Jun 30;4:e121. (PMID: 24979150)
      Basic Life Sci. 1992;59:693-8. (PMID: 1417695)
      PLoS One. 2018 May 25;13(5):e0197878. (PMID: 29799865)
      mSystems. 2017 Nov 14;2(6):. (PMID: 29152585)
      PLoS Comput Biol. 2015 May 07;11(5):e1004226. (PMID: 25950956)
      Animal. 2020 Jan;14(1):95-107. (PMID: 31571564)
      J Dairy Sci. 2020 Mar;103(3):2264-2271. (PMID: 31864747)
      BMC Res Notes. 2016 Jan 11;9:22. (PMID: 26754836)
      Anim Nutr. 2018 Jun;4(2):137-150. (PMID: 30140753)
      J Anim Sci. 2014 Jan;92(1):211-9. (PMID: 24243899)
      Sci Rep. 2018 Mar 28;8(1):5315. (PMID: 29593306)
      Front Microbiol. 2019 Jan 18;9:3335. (PMID: 30713531)
      Science. 2007 Jul 6;317(5834):58-62. (PMID: 17615333)
      Animals (Basel). 2020 Apr 27;10(5):. (PMID: 32349238)
      Microorganisms. 2020 Apr 15;8(4):. (PMID: 32326636)
      BMC Genomics. 2018 Nov 7;19(1):808. (PMID: 30404613)
      Cell Metab. 2017 Jan 10;25(1):140-151. (PMID: 27889387)
      Front Microbiol. 2018 Feb 13;9:224. (PMID: 29487593)
      Nat Protoc. 2020 Mar;15(3):799-821. (PMID: 31942082)
      Front Immunol. 2018 Jul 26;9:1704. (PMID: 30093904)
      Biomed Res Int. 2019 Dec 26;2019:6916189. (PMID: 31976326)
      PLoS One. 2014 Jun 18;9(6):e100040. (PMID: 24941112)
      Asian-Australas J Anim Sci. 2018 May;31(5):755-762. (PMID: 26954189)
      Arch Anim Nutr. 2010 Apr;64(2):121-35. (PMID: 20481351)
      Cell Host Microbe. 2016 Apr 13;19(4):443-54. (PMID: 27078066)
      Animals (Basel). 2020 Jan 23;10(2):. (PMID: 31979207)
      Microbiol Mol Biol Rev. 2006 Dec;70(4):939-1031. (PMID: 17158705)
      Microbiologyopen. 2019 Dec;8(12):e923. (PMID: 31496126)
      Molecules. 2020 Jan 30;25(3):. (PMID: 32019231)
      Animals (Basel). 2020 Apr 14;10(4):. (PMID: 32295190)
      Nucleic Acids Res. 2017 Jul 3;45(W1):W180-W188. (PMID: 28449106)
      Animals (Basel). 2020 Oct 22;10(11):. (PMID: 33105748)
      Gut. 2016 Mar;65(3):540-2. (PMID: 26276682)
    • Accession Number:
      0 (Butyrates)
      0 (DNA, Bacterial)
      0 (DNA, Ribosomal)
      0 (RNA, Ribosomal, 16S)
      0 (Tannins)
    • Publication Date:
      Date Created: 20210429 Date Completed: 20210930 Latest Revision: 20240401
    • Publication Date:
      20240401
    • Accession Number:
      PMC8084250
    • Accession Number:
      10.1371/journal.pone.0250874
    • Accession Number:
      33914832