Bovine coronavirus infections in Turkey: molecular analysis of the full-length spike gene sequences of viruses from digestive and respiratory infections.

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  • Additional Information
    • Source:
      Publisher: Springer-Verlag Country of Publication: Austria NLM ID: 7506870 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-8798 (Electronic) Linking ISSN: 03048608 NLM ISO Abbreviation: Arch Virol Subsets: MEDLINE
    • Publication Information:
      Original Publication: Wien, New York, Springer-Verlag.
    • Subject Terms:
      Cattle Diseases/*epidemiology ; Coronavirus Infections/*veterinary ; Coronavirus, Bovine/*genetics ; Diarrhea/*veterinary ; RNA, Viral/*genetics ; Respiratory Tract Infections/*veterinary ; Spike Glycoprotein, Coronavirus/*genetics; Agriculture ; Amino Acid Substitution ; Animals ; Cattle ; Cattle Diseases/virology ; Coronavirus Infections/epidemiology ; Coronavirus Infections/virology ; Coronavirus, Bovine/classification ; Diarrhea/epidemiology ; Diarrhea/virology ; Epidemiological Monitoring/veterinary ; Evolution, Molecular ; Feces/virology ; Humans ; Mutation ; Nasal Cavity/virology ; Phylogeny ; Respiratory Tract Infections/epidemiology ; Respiratory Tract Infections/virology ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Analysis, DNA ; Turkey/epidemiology
    • Abstract:
      Bovine coronavirus (BCoV) can be spread by animal activity. Although cattle farming is widespread in Turkey, there are few studies of BCoV. The aim of this study was to evaluate the current situation regarding BCoV in Turkey. This is the first study reporting the full-length nucleotide sequences of BCoV spike (S) genes in Turkey. Samples were collected from 119 cattle with clinical signs of respiratory (n = 78) or digestive tract (n = 41) infection on different farms located across widely separated provinces in Turkey. The samples were screened for BCoV using RT-nested PCR targeting the N gene, which identified BCoV in 35 samples (9 faeces and 26 nasal discharge). RT-PCR analysis of the S gene produced partial/full-length S gene sequences from 11 samples (8 faeces and 3 nasal discharge samples). A phylogenetic tree of the S gene sequences was made to analyze the genetic relationships among BCoVs from Turkey and other countries. The results showed that the local strains present in faeces and nasal discharge samples had many different amino acid changes. Some of these changes were shown in previous studies to be critical for tropism. This study provides new data on BCoV in Turkey that will be valuable in designing effective vaccine approaches and control strategies.
      (© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
    • References:
      Clin Diagn Lab Immunol. 2001 Mar;8(2):297-302. (PMID: 11238212)
      Nat Commun. 2020 Nov 26;11(1):6013. (PMID: 33243994)
      J Virol Methods. 2008 Aug;151(2):167-171. (PMID: 18579223)
      Vet Med Sci. 2018 Apr 24;:. (PMID: 29687958)
      J Gen Virol. 1990 Feb;71 ( Pt 2):487-92. (PMID: 2155300)
      Vet Clin North Am Food Anim Pract. 2010 Jul;26(2):349-64. (PMID: 20619189)
      J Virol. 1994 Sep;68(9):5403-10. (PMID: 7520090)
      Vet Microbiol. 2008 Jan 1;126(1-3):30-9. (PMID: 17669602)
      Virology. 1997 Jan 20;227(2):378-88. (PMID: 9018137)
      Acta Vet Hung. 2005;53(1):137-46. (PMID: 15782666)
      J Gen Virol. 2013 Sep;94(Pt 9):2036-2049. (PMID: 23804565)
      Genet Mol Res. 2017 Apr 05;16(2):. (PMID: 28387879)
      Infect Genet Evol. 2012 Dec;12(8):1870-8. (PMID: 22634277)
      Virology. 2001 Jan 20;279(2):371-4. (PMID: 11162792)
      Virus Genes. 1998;17(1):33-42. (PMID: 9778786)
      Bioinformatics. 2014 Nov 15;30(22):3276-8. (PMID: 25095880)
      Arch Virol. 2001 Dec;146(12):2401-19. (PMID: 11811688)
      Virology. 1991 Jan;180(1):395-9. (PMID: 1984658)
      J Clin Microbiol. 2006 Mar;44(3):957-60. (PMID: 16517883)
      Arch Virol. 2006 Sep;151(9):1735-48. (PMID: 16583154)
      Braz J Med Biol Res. 2008 Apr;41(4):277-82. (PMID: 18392449)
      Virus Genes. 2019 Jun;55(3):415-420. (PMID: 30771080)
      Virus Evol. 2017 Jun 12;3(1):vex012. (PMID: 28630747)
      J Virol. 1991 Nov;65(11):6232-7. (PMID: 1920630)
      Nat Struct Mol Biol. 2006 Aug;13(8):751-2. (PMID: 16845391)
      Virus Res. 2002 Mar 20;84(1-2):101-9. (PMID: 11900843)
      Ir Vet J. 2015 Dec 30;68:31. (PMID: 26719792)
      Vet Med Small Anim Clin. 1972 Feb;67(2):173-4 passim. (PMID: 4334051)
      Lancet. 2020 Feb 15;395(10223):497-506. (PMID: 31986264)
      Arch Virol. 2007;152(10):1885-900. (PMID: 17564760)
      Viruses. 2019 Jan 14;11(1):. (PMID: 30646565)
      Methods Mol Biol. 2015;1282:1-23. (PMID: 25720466)
      Transbound Emerg Dis. 2011 Apr;58(2):179-85. (PMID: 26353053)
    • Grant Information:
      18L0239002 Ankara Universitesi
    • Accession Number:
      0 (RNA, Viral)
      0 (Spike Glycoprotein, Coronavirus)
    • Publication Date:
      Date Created: 20210702 Date Completed: 20210804 Latest Revision: 20220716
    • Publication Date:
      20240105
    • Accession Number:
      PMC8247624
    • Accession Number:
      10.1007/s00705-021-05147-2
    • Accession Number:
      34212242