Effect of Hepatocyte Growth Factor-Transfected Human Umbilical Cord Mesenchymal Stem Cells on Hepatic Stellate Cells by Regulating Transforming Growth Factor-β1/Smads Signaling Pathway.

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  • Author(s): Yin F;Yin F; Mao LC; Mao LC; Cai QQ; Cai QQ; Jiang WH; Jiang WH
  • Source:
    Stem cells and development [Stem Cells Dev] 2021 Nov 01; Vol. 30 (21), pp. 1070-1081. Date of Electronic Publication: 2021 Oct 22.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 101197107 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1557-8534 (Electronic) Linking ISSN: 15473287 NLM ISO Abbreviation: Stem Cells Dev Subsets: MEDLINE
    • Publication Information:
      Original Publication: Larchmont, NY : Mary Ann Liebert, Inc., c2004-
    • Subject Terms:
      Hepatic Stellate Cells*/metabolism ; Hepatocyte Growth Factor*/administration & dosage ; Hepatocyte Growth Factor*/metabolism ; Mesenchymal Stem Cell Transplantation* ; Mesenchymal Stem Cells* ; Smad Proteins*/metabolism ; Transforming Growth Factor beta1*/metabolism; Cell Proliferation ; Humans ; Liver Cirrhosis/metabolism ; Liver Cirrhosis/therapy ; Signal Transduction ; Umbilical Cord
    • Abstract:
      Studies have shown that human umbilical cord mesenchymal stem cells (hUCMSCs) could ameliorate liver fibrosis (LF) through inhibiting the activation of hepatic stellate cells (HSCs). However, the specific mechanisms have not been studied clearly. The purpose of this study was to explore the possible mechanism of hepatocyte growth factor (HGF)-transfected hUCMSCs in inhibiting the proliferation and activation of HSCs-T6. The upper and lower double-cell coculture system was established among HGF-hUCMSCs, LV5-NC-hUCMSCs, hUCMSCs, and HSCs-T6 in experimental groups; HSCs-T6 were cultured alone as control group. After coculturing for 1, 2, and 3 days, results showed that HGF-transfected hUCMSCs could decrease cell viability of HSCs-T6 and promote apoptosis; inhibit their activation and reduce the expression of Collagen I, Collagen III, TGF-β1, Smad2 and Smad3, which may be related to inhibiting the activation of TGF-β1/Smads signaling pathway. These findings suggested that HGF-transfected hUCMSCs may be used as an alternative and novel therapeutic approach for the treatment of LF.
    • Contributed Indexing:
      Keywords: HGF; TGF-β1/Smads signaling pathway; coculture; hepatic stellate cells-T6; human umbilical cord mesenchymal stem cells; liver fibrosis
    • Accession Number:
      0 (Smad Proteins)
      0 (Transforming Growth Factor beta1)
      67256-21-7 (Hepatocyte Growth Factor)
    • Publication Date:
      Date Created: 20210913 Date Completed: 20220321 Latest Revision: 20220531
    • Publication Date:
      20240105
    • Accession Number:
      10.1089/scd.2021.0136
    • Accession Number:
      34514810