Optimization Method for Phenolic Compounds Extraction from Medicinal Plant ( Juniperus procera) and Phytochemicals Screening.

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  • Additional Information
    • Source:
      Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE
    • Publication Information:
      Original Publication: Basel, Switzerland : MDPI, c1995-
    • Subject Terms:
    • Abstract:
      Juniperus procera is a natural source of bioactive compounds with the potential of antitumor, antimicrobial, insecticidal, antifungal, and antioxidant activities. An optimization method was developed for total phenolic content (TPC), total flavonoid content (TFC), and total tannin content (TTC) in leaf and seed extract of Juniperus procera. Organic solvents (methanol (99.8%), ethanol (99%), and acetone (99.5%)), and deionized water (DI) were used for extraction. The estimation of TPC, TFC, and TTC in plant materials was carried out using UV-spectrophotometer and HPLC with the standards gallic acid, quercetin, and tannic acid. Recovery of TPC in leaf extract ranged from 2.9 to 9.7 mg GAE/g DW, TFC from 0.9 to 5.9 mg QE/g DW, and TTC ranged from 1.5 to 4.3 mg TA/g DW while the TPC value in the seed extract ranged from 0.53 to 2.6 mg GAE/g DW, TFC from 0.5 to 1.6 mg QE/g DW, and TTC ranged from 0.5 to 1.4 mg TA/g DW. This result revealed that methanol is the best solvent for recovery of the TPC value (9.7 mg) from leaf extract in comparison to other solvents. Ethanol recorded the highest result of TFC (5.9 mg) in leaf extract among the solvents whereas acetone was the best for TTC yield recovery from leaf extract (4.3 mg). In the case of the seed extract, ethanol was the best solvent for both TPC (2.6 mg), and TFC (1.6 mg) recovery in comparison to other solvents. Total tannin content in methanol resulted in significant recovery from seed extract (1.4 mg). Separation and quantification of gallic acid, quercetin, and tannic acid in plant materials were undertaken using HPLC. Gallic acid in leaf and seed of J. procera ranged from 6.6 to 9.2, 6.5 to 7.2 µg/g DW, quercetin from 6.3 to 18.2, 0.9 to 4.2 µg/g DW, and tannic acid from 16.2 to 29.3, 6.6 to 9.3 µg/g DW, respectively. Solvents have shown a significant effect in the extraction of phenolic compounds. Moreover, phytochemicals in plant materials were identified using GC-MS and resulted in very important bioactive compounds, which include anti-inflammatory, antibacterial, and antitumor agents such as ferruginol, phenanthrene, and n-hexadecanoic acid. In conclusion, the optimal solvent for extraction depends on the part of the plant material and the compounds that are to be isolated.
    • References:
      Curr Drug Metab. 2018;19(3):236-263. (PMID: 29380697)
      Farmaco. 2000 Sep-Oct;55(9-10):619-23. (PMID: 11152243)
      Methods Mol Biol. 2012;864:427-64. (PMID: 22367907)
      Chem Biol Drug Des. 2012 Sep;80(3):434-9. (PMID: 22642495)
      Bioorg Med Chem. 2005 Dec 15;13(24):6685-92. (PMID: 16125391)
      Crit Rev Anal Chem. 2016 May 3;46(3):257-65. (PMID: 26440222)
      Int J Mol Sci. 2016 Feb 18;17(2):160. (PMID: 26901191)
      J Agric Food Chem. 2016 Sep 28;64(38):7049-58. (PMID: 27615454)
      Chem Sci. 2017 Feb 1;8(2):1406-1413. (PMID: 28616142)
      Phytother Res. 2012 Feb;26(2):273-6. (PMID: 21674631)
      Anc Sci Life. 2012 Apr;31(4):198-201. (PMID: 23661869)
      J Chromatogr A. 2004 Oct 29;1054(1-2):95-111. (PMID: 15553136)
      Viruses. 2016 Jun 06;8(6):. (PMID: 27275830)
      Materials (Basel). 2020 Jul 20;13(14):. (PMID: 32698426)
      Curr Res Food Sci. 2021 Apr 02;4:200-214. (PMID: 33899007)
      Biomed Chromatogr. 2009 Oct;23(10):1116-20. (PMID: 19444795)
      J Chromatogr Sci. 2013 Oct;51(9):883-90. (PMID: 23135132)
      J Toxicol. 2011;2011:494168. (PMID: 21776261)
      J Chromatogr A. 2003 Nov 7;1018(1):29-40. (PMID: 14582624)
      Mol Med Rep. 2017 Nov;16(5):7013-7017. (PMID: 28901510)
      Antibiotics (Basel). 2020 May 19;9(5):. (PMID: 32438609)
      Biomed Pharmacother. 2021 Jan;133:110985. (PMID: 33212373)
      Medicines (Basel). 2018 Aug 25;5(3):. (PMID: 30149600)
      Biochim Biophys Acta. 1997 Oct 20;1336(3):509-21. (PMID: 9367179)
      J Sex Med. 2015 Dec;12(12):2401-12. (PMID: 26597311)
      J Food Sci. 2007 Mar;72(2):S159-66. (PMID: 17995858)
      J Invest Dermatol. 2013 Feb;133(2):377-85. (PMID: 23014338)
      Molecules. 2011 Aug 10;16(8):6806-15. (PMID: 21832971)
      Nat Protoc. 2007;2(4):875-7. (PMID: 17446889)
      J Org Chem. 2018 Oct 5;83(19):11804-11813. (PMID: 30141629)
      Steroids. 2017 Mar;119:43-52. (PMID: 28143704)
      Expert Opin Ther Pat. 2010 Oct;20(10):1295-319. (PMID: 20645884)
      Life (Basel). 2020 Sep 28;10(10):. (PMID: 32998223)
      Sci Rep. 2021 Sep 27;11(1):19136. (PMID: 34580362)
      Biotechnol Rep (Amst). 2020 Jun 25;27:e00496. (PMID: 32637346)
      Biotechnol Adv. 2015 Dec;33(8):1582-1614. (PMID: 26281720)
      J Chromatogr A. 2011 May 6;1218(18):2505-12. (PMID: 21411105)
      Drug Alcohol Depend. 2017 Apr 01;173:59-68. (PMID: 28199917)
      Toxins (Basel). 2021 Jul 16;13(7):. (PMID: 34357967)
      Molecules. 2010 Dec 03;15(12):8813-26. (PMID: 21131901)
      BMC Plant Biol. 2021 Apr 21;21(1):192. (PMID: 33882830)
      Asian Pac J Trop Biomed. 2012 Dec;2(12):960-5. (PMID: 23593576)
      J Sep Sci. 2007 Dec;30(18):3268-95. (PMID: 18069740)
      Phytomedicine. 2017 Jan 15;24:157-165. (PMID: 28160857)
      J Antimicrob Chemother. 2001 Oct;48(4):487-91. (PMID: 11581226)
      Bioorg Med Chem Lett. 2014 Nov 15;24(22):5234-7. (PMID: 25316317)
      Molecules. 2010 Oct 21;15(10):7313-52. (PMID: 20966876)
      Molecules. 2013 Feb 19;18(2):2328-75. (PMID: 23429347)
      Antioxidants (Basel). 2020 Dec 12;9(12):. (PMID: 33322700)
    • Grant Information:
      RSP-2021/73 The authors extend their appreciation to Researchers supporting project number (RSP-2021/73) at king Saud University, Riyadh, Saudi Arabia.
    • Contributed Indexing:
      Keywords: chromatography analysis; flavonoid; new approach; phytochemicals profiling; solvents; spectrophotometry; tannin
    • Accession Number:
      0 (Antioxidants)
      0 (Flavonoids)
      0 (Phenols)
      0 (Phytochemicals)
      0 (Plant Extracts)
    • Publication Date:
      Date Created: 20211224 Date Completed: 20220211 Latest Revision: 20220211
    • Publication Date:
      20240104
    • Accession Number:
      PMC8708409
    • Accession Number:
      10.3390/molecules26247454
    • Accession Number:
      34946537