Coenzyme Q10 reduces expression of apoptotic markers in adult rat nucleus accumbens dopaminergic neurons treated with methamphetamine.

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    • Source:
      Publisher: Reidel Country of Publication: Netherlands NLM ID: 0403234 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-4978 (Electronic) Linking ISSN: 03014851 NLM ISO Abbreviation: Mol Biol Rep Subsets: MEDLINE
    • Publication Information:
      Original Publication: Dordrecht, Boston, Reidel.
    • Subject Terms:
    • Abstract:
      Background: Abuse of addictive drugs such as methamphetamine (METH) has become a global problem, leading to many social, economic, and health disturbances, including neurological and cognitive disorders. Neuronal damage is reported in chronic METH abusers. The neuroprotective role of CoQ10 has been shown in many studies. In the present study, we aimed to assess the pre and post-efficacy of CoQ10 on the dopaminergic neurons of the Nucleus Accumbens (de Miranda et al. in Food Res Int 121:641-647, 2019) in the male adult rats treated with METH.
      Methods: 80 rats were randomly divided into eight groups (n = 10), including: negative control (intact), positive control (received 5 mg/kg/day METH/IP), three post-treatment groups (METH + 5, 10, 20 mg/kg CoQ10) and three pre-treatment groups (received 5, 10, 20 mg/kg CoQ10 as pre-treatment for 14 days before METH injection). The expression of Bax, Bcl-2, Bax/Bcl-2 ratio, P53, Caspase-3 and tyrosine hydroxylase in NAc studied using western blotting. Nissl staining was used to study the neuronal density of NAc.
      Results: Our results showed that the different doses of CoQ10 in METH-treated animals significantly changed pro-apoptotic proteins' expression in the benefit of neuronal survival of NAc (P < 0.05). Neuronal density in NAc were significantly lower in the METH group compared to the control and CoQ10 treated groups. Pre- and post-treatment with different doses of CoQ10 restored the neuronal damage in NAc.
      Conclusions: CoQ10 could decrease the activation of pro-apoptotic proteins and reduce the neurodegenerative effects induced by METH. From a clinical point of view, it seems that certain antioxidants such as CoQ10 should receive more attention in clinical trial research. We believe that antioxidants could be the promising for drug abuse treatment in the future.
      (© 2021. The Author(s), under exclusive licence to Springer Nature B.V.)
    • References:
      Peacock A, Leung J, Larney S, Colledge S, Hickman M, Rehm J et al (2018) Global statistics on alcohol, tobacco and illicit drug use: 2017 status report. Addiction 113(10):1905–1926. (PMID: 10.1111/add.14234)
      Potvin S, Pelletier J, Grot S, Hebert C, Barr AM, Lecomte T (2018) Cognitive deficits in individuals with methamphetamine use disorder: a meta-analysis. Addict Behav 80:154–160. (PMID: 10.1016/j.addbeh.2018.01.021)
      Turowski P, Kenny B-A (2015) The blood-brain barrier and methamphetamine: open sesame? Front NeuroSci 9:156. (PMID: 10.3389/fnins.2015.00156)
      Thrash B, Karuppagounder SS, Uthayathas S, Suppiramaniam V, Dhanasekaran M (2010) Neurotoxic effects of methamphetamine. Neurochem Res 35(1):171–179. (PMID: 10.1007/s11064-009-0042-5)
      Rocha A, Kalivas PW (2010) Role of the prefrontal cortex and nucleus accumbens in reinstating methamphetamine seeking. Eur J Neurosci 31(5):903–909. (PMID: 10.1111/j.1460-9568.2010.07134.x)
      Moszczynska A, Callan SP (2017) Molecular, behavioral, and physiological consequences of methamphetamine neurotoxicity: implications for treatment. J Pharmacol Exp Ther 362(3):474–488. (PMID: 10.1124/jpet.116.238501)
      Moszczynska A, Yamamoto BK (2011) Methamphetamine oxidatively damages parkin and decreases the activity of 26S proteasome in vivo. J Neurochem 116(6):1005–1017. (PMID: 10.1111/j.1471-4159.2010.07147.x)
      Limanaqi F, Gambardella S, Biagioni F, Busceti CL, Fornai F (2018) Epigenetic effects induced by methamphetamine and methamphetamine-dependent oxidative stress. Oxidative Med Cell Longev. https://doi.org/10.1155/2018/4982453. (PMID: 10.1155/2018/4982453)
      Frank MG, Adhikary S, Sobesky JL, Weber MD, Watkins LR, Maier SF (2016) The danger-associated molecular pattern HMGB1 mediates the neuroinflammatory effects of methamphetamine. Brain Behav Immun 51:99–108. (PMID: 10.1016/j.bbi.2015.08.001)
      Halpin LE, Collins SA, Yamamoto BK (2014) Neurotoxicity of methamphetamine and 3, 4-methylenedioxymethamphetamine. Life Sci 97(1):37–44. (PMID: 10.1016/j.lfs.2013.07.014)
      Chen C, Qincao L, Xu J, Du S, Huang E, Liu C et al (2016) Role of PUMA in methamphetamine-induced neuronal apoptosis. Toxicol Lett 240(1):149–160. (PMID: 10.1016/j.toxlet.2015.10.020)
      Elyasi L, Eftekhar-Vaghefi SH, Esmaeili-Mahani S (2014) Morphine protects SH-SY5Y human neuroblastoma cells against 6-hydroxydopamine–induced cell damage: involvement of anti-oxidant, calcium blocking, and anti-apoptotic properties. Rejuven Res 17(3):255–263. (PMID: 10.1089/rej.2013.1473)
      Nopparat C, Porter JE, Ebadi M, Govitrapong P (2010) The mechanism for the neuroprotective effect of melatonin against methamphetamine-induced autophagy. J Pineal Res 49(4):382–389. (PMID: 10.1111/j.1600-079X.2010.00805.x)
      Bank G, Kagan D, Madhavi D (2011) Coenzyme Q10: clinical update and bioavailability. J Evid-Based Complement Altern Med 16(2):129–137. (PMID: 10.1177/2156587211399438)
      Yang X, Zhang Y, Xu H, Luo X, Yu J, Liu J et al (2016) Neuroprotection of coenzyme Q10 in neurodegenerative diseases. Curr Top Med Chem 16(8):858–866. (PMID: 10.2174/1568026615666150827095252)
      Thanh HN, Minh HPT, Duc LV, Thanh TB (2016) Protective effect of coenzyme Q10 on methamphetamine-induced neurotoxicity in the mouse brain. Trends Med Res 11(1):1–10. (PMID: 10.3923/tmr.2016.1.10)
      Klongpanichapak S, Govitrapong P, Sharma SK, Ebadi M (2006) Attenuation of cocaine and methamphetamine neurotoxicity by coenzyme Q 10. Neurochem Res 31(3):303–311. (PMID: 10.1007/s11064-005-9025-3)
      Gholipour F, Shams J, Zahiroddin A (2017) Protective effect of coenzyme Q10 on methamphetamine-induced apoptosis in adult male rats. Nov Biomed 5(3):127–132.
      Jameie SB, Masoumipoor M, Janzadeh A, Nasirinezhad F, Kerdari M, Soleimani M (2014) Combined therapeutic effects of low power laser (980 nm) and CoQ10 on Neuropathic Pain in adult male rat. Med J Islam Repub Iran 28:58. (PMID: 254051244219887)
      Björklund A, Dunnett SB (2019) The amphetamine induced rotation test: a re-assessment of its use as a tool to monitor motor impairment and functional recovery in rodent models of Parkinson’s disease. J Parkinson’s Dis 9(1):17–29. (PMID: 10.3233/JPD-181525)
      Thanos PK, Kim R, Delis F, Ananth M, Chachati G, Rocco MJ et al (2016) Chronic methamphetamine effects on brain structure and function in rats. PLoS One 11(6):e0155457. (PMID: 10.1371/journal.pone.0155457)
      Hori N, Kadota M, Watanabe M, Ito Y, Akaike N, Carpenter D (2010) Neurotoxic effects of methamphetamine on rat hippocampus pyramidal neurons. Cell Mol Neurobiol 30(6):849–856. (PMID: 10.1007/s10571-010-9512-1)
      Martin TA, Jayanthi S, McCoy MT, Brannock C, Ladenheim B, Garrett T et al (2012) Methamphetamine causes differential alterations in gene expression and patterns of histone acetylation/hypoacetylation in the rat nucleus accumbens. PLoS One 7(3):e34236. (PMID: 10.1371/journal.pone.0034236)
      Yu S, Zhu L, Shen Q, Bai X, Di X (2015) Recent advances in methamphetamine neurotoxicity mechanisms and its molecular pathophysiology. Behav Neurol. https://doi.org/10.1155/2015/103969. (PMID: 10.1155/2015/1039692655773826557738)
      Zhou F, Yang Y, Xing D (2011) Bcl-2 and Bcl‐xL play important roles in the crosstalk between autophagy and apoptosis. FEBS J 278(3):403–413. (PMID: 10.1111/j.1742-4658.2010.07965.x)
      Huang W, Xie W-B, Qiao D, Qiu P, Huang E, Li B et al (2015) Caspase-11 plays an essential role in methamphetamine-induced dopaminergic neuron apoptosis. Toxicol Sci 145(1):68–79. (PMID: 10.1093/toxsci/kfv014)
      Parrish AB, Freel CD, Kornbluth S (2013) Cellular mechanisms controlling caspase activation and function. Cold Spring Harb Perspect Biol 5(6):a008672. (PMID: 10.1101/cshperspect.a008672)
      Reiner DJ, Yu S-J, Shen H, He Y, Bae E, Wang Y (2014) 9-Cis retinoic acid protects against methamphetamine-induced neurotoxicity in nigrostriatal dopamine neurons. Neurotox Res 25(3):248–261. (PMID: 10.1007/s12640-013-9413-4)
      Bentinger M, Tekle M, Dallner G (2010) Coenzyme Q–biosynthesis and functions. Biochem Biophys Res Commun 396(1):74–79. (PMID: 10.1016/j.bbrc.2010.02.147)
      Badawy Khair NS, Mohammed SA (2021) A comparative study on the protective role of Silymarin and Coenzyme-Q10 on the cerebellar cortex of experimentally induced atherosclerosis in adult male albino rats: a histological, immunohistochemical and biochemical study. Egypt J Histol 44(2):322–338.
      Mancuso M, Orsucci D, Volpi L, Calsolaro V, Siciliano G (2010) Coenzyme Q10 in neuromuscular and neurodegenerative disorders. Curr Drug Targets 11(1):111–121. (PMID: 10.2174/138945010790031018)
      Spindler M, Beal MF, Henchcliffe C (2009) Coenzyme Q10 effects in neurodegenerative disease. Neuropsychiatr Dis Treat 5:597. (PMID: 199669072785862)
      Vaselbehagh M, Sadegh M, Karami H, Babaie S (2021) Coenzyme Q10 modulates apoptotic effects of chronic administration of methadone on NMRI mouse hippocampus. Cell J (Yakhteh) 23:538–5435.
    • Contributed Indexing:
      Keywords: Apoptotic markers; CoQ10; Methamphetamine; Nucleus Accumbens
    • Accession Number:
      1339-63-5 (Ubiquinone)
      44RAL3456C (Methamphetamine)
      EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
      EJ27X76M46 (coenzyme Q10)
    • Publication Date:
      Date Created: 20220116 Date Completed: 20220331 Latest Revision: 20220401
    • Publication Date:
      20240105
    • Accession Number:
      10.1007/s11033-021-07049-7
    • Accession Number:
      35034284