Strategies for Enhancing the Homology-Directed Repair Efficiency of CRISPR-Cas Systems.

Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 101738191 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2573-1602 (Electronic) Linking ISSN: 25731599 NLM ISO Abbreviation: CRISPR J Subsets: MEDLINE

Original Publication: [New Rochelle, NY] : Mary Ann Liebert, Inc., [2018]-

CRISPR-Cas Systems*/genetics ; Gene Editing*; DNA End-Joining Repair/genetics ; Endonucleases/genetics ; Recombinational DNA Repair

The CRISPR-Cas nuclease has emerged as a powerful genome-editing tool in recent years. The CRISPR-Cas system induces double-strand breaks that can be repaired via the non-homologous end joining or homology-directed repair (HDR) pathway. Compared to non-homologous end joining, HDR can be used for the treatment of incurable monogenetic diseases. Therefore, remarkable efforts have been dedicated to enhancing the efficacy of HDR. In this review, we summarize the currently used strategies for enhancing the HDR efficiency of CRISPR-Cas systems based on three factors: (1) regulation of the key factors in the DNA repair pathways, (2) modulation of the components in the CRISPR machinery, and (3) alteration of the intracellular environment around double-strand breaks. Representative cases and potential solutions for further improving HDR efficiency are also discussed, facilitating the development of new CRISPR technologies to achieve highly precise genetic manipulation in the future.

EC 3.1.- (Endonucleases)

Date Created: 20220125 Date Completed: 20220404 Latest Revision: 20220405

20240105

10.1089/crispr.2021.0039

35076280