Wound healing properties of pharmaceutical gel containing isopimarane diterpene isolated from Kaempferia galanga L.

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  • Additional Information
    • Source:
      Publisher: Elsevier Sequoia Country of Publication: Ireland NLM ID: 7903310 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7573 (Electronic) Linking ISSN: 03788741 NLM ISO Abbreviation: J Ethnopharmacol Subsets: MEDLINE
    • Publication Information:
      Publication: Limerick : Elsevier Sequoia
      Original Publication: Lausanne, Elsevier Sequoia.
    • Subject Terms:
      Abietanes/*pharmacology ; Oxidative Stress/*drug effects ; Wound Healing/*drug effects ; Zingiberaceae/*chemistry; Abietanes/administration & dosage ; Abietanes/isolation & purification ; Animals ; Anti-Inflammatory Agents/administration & dosage ; Anti-Inflammatory Agents/isolation & purification ; Anti-Inflammatory Agents/pharmacology ; Cell Line ; Cell Survival/drug effects ; Fibroblasts/drug effects ; Fibroblasts/pathology ; Gels ; Humans ; Hydrogen Peroxide ; Mice ; Nitric Oxide/metabolism ; Plant Preparations/pharmacology ; RAW 264.7 Cells ; Rhizome
    • Abstract:
      Ethnopharmacological Relevance: Kaempferia galanga L. rhizomes have been widely used in Thailand as medicine for treating inflammation and wound. A number of bioactive compounds have been isolated from the rhizomes of K. galanga and these compounds exhibited various pharmacological activities.
      Aim of the Study: The objective of this study is to investigate the wound healing properties of gel containing 6β-acetoxysandaracopimaradiene-1α, 9α-diol (KG6), a compound from K. galanga.
      Materials and Methods: KG6 gel formulations were prepared using 1.0% carbopol 940 as gelling agent. Three KG6 gel formulations (0.10, 0.25, 0.50% w/w) were subjected to heating-cooling test to determine their physical, chemical and biological stabilities. The wound healing properties of KG6 gel formulations were performed using RAW264.7 cells for anti-inflammatory effect, while their impact on cell proliferation and migration, collagen content and H 2 O 2 -induced oxidative stress was examined using human dermal fibroblasts (HDF).
      Results: The pH, viscosity and general appearance after the heating-cooling test of the three prepared gels were stable in the acceptable range of gel formulation for skin. Gel containing 0.25% KG6 showed better chemical stability than other formulations. The 0.25% KG6 gel significantly increased cell viability (102.8%) and produced the highest HDF cell migration (91.9%) which was greater than that of Aloe vera gel (96.2, 78.4%, respectively). This gel exhibited anti-inflammatory activity via suppressing nitric oxide release and improved the viability of HDF cells against H 2 O 2 -induced oxidative stress. The 0.25% KG6 gels also increased collagen content in HDF cells.
      Conclusion: The gel formulation consisting of 0.25% KG6 with 1.0% of carbopol 940 was found to be a promising pharmaceutical gel for wound treatments due to marked wound healing properties.
      (Copyright © 2022 Elsevier B.V. All rights reserved.)
    • Contributed Indexing:
      Keywords: Isopimarane diterpenes; Kaempferia galanga; Wound healing activity
    • Accession Number:
      0 (Abietanes)
      0 (Aloe vera gel)
      0 (Anti-Inflammatory Agents)
      0 (Gels)
      0 (Plant Preparations)
      31C4KY9ESH (Nitric Oxide)
      BBX060AN9V (Hydrogen Peroxide)
    • Publication Date:
      Date Created: 20220201 Date Completed: 20220314 Latest Revision: 20220314
    • Publication Date:
      20240104
    • Accession Number:
      10.1016/j.jep.2022.115052
    • Accession Number:
      35101569