Changes in Cytochrome C Oxidase Redox State and Hemoglobin Concentration in Rat Brain During 810 nm Irradiation Measured by Broadband Near-Infrared Spectroscopy.

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  • Author(s): Oh Y;Oh Y; Nguyen N; Nguyen N; Jung HJ; Jung HJ; Choe Y; Choe Y; Kim JG; Kim JG
  • Source:
    Photobiomodulation, photomedicine, and laser surgery [Photobiomodul Photomed Laser Surg] 2022 May; Vol. 40 (5), pp. 315-324. Date of Electronic Publication: 2022 Apr 28.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 101734781 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2578-5478 (Electronic) Linking ISSN: 25785478 NLM ISO Abbreviation: Photobiomodul Photomed Laser Surg Subsets: MEDLINE
    • Publication Information:
      Original Publication: [New Rochelle, NY] : Mary Ann Liebert, Inc., [2019]-
    • Subject Terms:
      Isoflurane*/metabolism ; Isoflurane*/pharmacology ; Spectroscopy, Near-Infrared*/methods; Animals ; Brain/radiation effects ; Electron Transport Complex IV/metabolism ; Hemoglobins/metabolism ; Oxidation-Reduction ; Rats ; Rats, Sprague-Dawley
    • Abstract:
      Objective: The effects of 810 nm light-emitting diode (LED) photobiomodulation (PBM) on cerebral metabolism and cerebral hemodynamic were investigated by using a broadband near-infrared spectroscopy (bb-NIRS) under anesthesia conditions with isoflurane. Background data: PBM was supposed to increase cerebral hemodynamic and cerebral metabolism. There has been no study about the effect of 810 nm LED stimulation on cerebral hemodynamic and cerebral metabolism in vivo by using bb-NIRS measurement. Methods: PBM was applied to seven Sprague-Dawley rats at 50 mW/cm 2 power density. The changes in hemoglobin concentration ( Δ H b O 2 and Δ H H b ) and oxidized cytochrome c oxidase (CCO) concentration ( Δ o x C C O ) were measured using a bb-NIRS. The total hemoglobin and the difference in hemoglobin concentration changes were calculated by summation and difference of Δ H b O 2 and Δ H H b , respectively. Results: PBM evoked the gradual increases of Δ H b O 2 (+7.7 μM vs. baseline, p  = 0.008), Δ H b T (+9.5 μM vs. baseline, p  = 0.0044), and Δ H b D (+5.9 μM vs. baseline, p  > 0.05) during light stimulation. Meanwhile, Δ o x C C O (-3.5 μM vs. baseline, p  = 0.0019) was significantly decreased right after the onset of stimulation. Conclusions: PBM with 810 nm LED (50 mW/cm 2 ) increased cerebral oxygenation and blood volume as expected. However, oxidized CCO concentration was decreased, which was contrary to most previous studies. The two pathways of PBM effects on mitochondria and the inhibition of complex I by isoflurane were suggested to explain the decreased Δ o x C C O during PBM, but further study is required for the verification.
    • Contributed Indexing:
      Keywords: 810 nm LED; bb-NIRS; cerebral hemodynamic; cerebral metabolism; photobiomodulation
    • Accession Number:
      0 (Hemoglobins)
      CYS9AKD70P (Isoflurane)
      EC 1.9.3.1 (Electron Transport Complex IV)
    • Publication Date:
      Date Created: 20220428 Date Completed: 20220517 Latest Revision: 20220517
    • Publication Date:
      20240104
    • Accession Number:
      10.1089/photob.2021.0089
    • Accession Number:
      35483096