Proteomic Analysis of Retinal Conditioned Medium: The Effect on Early Differentiation of Embryonic Stem Cells into Retina.

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  • Additional Information
    • Source:
      Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 101197107 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1557-8534 (Electronic) Linking ISSN: 15473287 NLM ISO Abbreviation: Stem Cells Dev Subsets: MEDLINE
    • Publication Information:
      Original Publication: Larchmont, NY : Mary Ann Liebert, Inc., c2004-
    • Subject Terms:
      Proteomics* ; Embryonic Stem Cells*; Animals ; Mice ; Culture Media, Conditioned/pharmacology ; Culture Media, Conditioned/metabolism ; Cell Differentiation ; Retina/metabolism
    • Abstract:
      Stem cell replacement therapy has emerged as one of the most promising treatment options for retinal degenerative diseases, which are the main causes of irreversible vision loss. Three-dimensional (3D) retinal organoid culture is a cutting-edge technology for differentiating embryonic stem cells into retinal cells by forming a laminated retinal structure. However, 3D culture systems have strict requirements with respect to the experimental environment and culture technologies. Our study aimed to investigate the effect of retinal conditioned medium (RCM) at different developmental stages on the early differentiation of embryonic stem cells into retina in a 3D culture system. In this study, we added RCM to the 3D culture system and found that it could promote the differentiation of mouse embryonic stem cells (mESCs) into neuroretina. We further explored the possible mechanisms of RCM that regulate differentiation through proteomic analysis. RCM at different time points disclosed different protein profiles. Proteins which improved energy metabolism of mESCs might help improve the viability of embryonic bodies. We then screened out Snap25 , Cntn1 , Negr1 , Dpysl2 , Dpysl3 , and Crmp1 as candidate proteins that might play roles in the differentiation and neurogenesis processes of mESCs, hoping to provide a basis for optimizing a retinal differentiation protocol from embryonic stem cells.
    • Contributed Indexing:
      Keywords: differentiation; mouse embryonic stem cell; proteomic analysis; retinal conditioned medium
    • Accession Number:
      0 (Culture Media, Conditioned)
    • Publication Date:
      Date Created: 20220602 Date Completed: 20221111 Latest Revision: 20221214
    • Publication Date:
      20240105
    • Accession Number:
      10.1089/scd.2022.0067
    • Accession Number:
      35652355