Occurrence of bla NDM-7 and association with bla KPC-2 , bla CTX-M15 , aac, aph, mph(A), catB3 and virulence genes in a clinical isolate of Klebsiella pneumoniae with different plasmids in Brazil.

Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 0410427 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-072X (Electronic) Linking ISSN: 03028933 NLM ISO Abbreviation: Arch Microbiol Subsets: MEDLINE

Original Publication: Berlin, New York, Springer-Verlag.

Klebsiella Infections* ; Klebsiella pneumoniae*/genetics; Brazil ; Drug Resistance, Multiple, Bacterial/genetics ; Galanin/analogs & derivatives ; Humans ; Plasmids/genetics ; Sequence Analysis, DNA ; Substance P/analogs & derivatives ; Virulence/genetics ; beta-Lactamases/genetics

To characterize phenotypically and genotypically an isolate of multidrug-resistant (MDR) K. pneumoniae from a patient with septicemia in a hospital in Recife-PE, Brazil, resistance and virulence genes were investigated using PCR and sequencing the amplicons, and the plasmid DNA was also sequenced. The K74-A3 isolate was resistant to all β-lactams, including carbapenems, as well as to aminoglycosides and quinolones. By conducting a PCR analysis and sequencing, the variants bla NDM-7 associated with bla KPC-2 and the cps, wabG, fim-H, mrkD and entB virulence genes were identified. The analysis of plasmid revealed the presence of bla CTX-M15 , aac(3)-IVa, aph(3')-Ia, aph(4)-Ia, aac(6')ib-cr, mph(A) and catB3, and also the plasmids IncX3, IncFIB, IncQ1, ColRNAI and ColpVC. To our knowledge, this is the first report of the bla NDM-7 gene in Recife-PE and we suggest that this variant is located in IncX3. These results alert us to the risk of spreading an isolate with a vast genetic arsenal of resistance, in addition to which several plasmids are present that favor the horizontal transfer of these genes.
(© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

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Keywords: Brazil; INCX3; Klebsiella pneumoniae; Resistance genes; Virulence genes; bla NDM-7

138579-66-5 (galantide)
33507-63-0 (Substance P)
88813-36-9 (Galanin)
EC 3.5.2.6 (beta-Lactamases)

Date Created: 20220705 Date Completed: 20220706 Latest Revision: 20220706

20240105

10.1007/s00203-022-03051-0

35788427