Menu
×
West Ashley Library
9 a.m. – 7 p.m.
Phone: (843) 766-6635
Main Library
9 a.m. - 8 p.m.
Phone: (843) 805-6930
Folly Beach Library
Closed for renovations
Phone: (843) 588-2001
John L. Dart Library
9 a.m. – 7 p.m.
Phone: (843) 722-7550
St. Paul's/Hollywood Library
9 a.m. - 8 p.m.
Phone: (843) 889-3300
Mt. Pleasant Library
9 a.m. – 8 p.m.
Phone: (843) 849-6161
Dorchester Road Library
9 a.m. - 8 p.m.
Phone: (843) 552-6466
Edgar Allan Poe/Sullivan's Island Library
9 a.m. - 6 p.m.
Phone: (843) 883-3914
John's Island Library
9 a.m. – 8 p.m.
Phone: (843) 559-1945
McClellanville Library
Closed for renovations
Phone: (843) 887-3699
Edisto Library
9 a.m. - 6 p.m.
Phone: (843) 869-2355
Wando Mount Pleasant Library
9 a.m. - 8 p.m.
Phone: (843) 805-6888
Otranto Road Library
9 a.m. - 8 p.m.
Phone: (843) 572-4094
Hurd/St. Andrews Library
9 a.m. - 8 p.m.
Phone: (843) 766-2546
Baxter-Patrick James Island
9 p.m. - 8 p.m.
Phone: (843) 795-6679
Bees Ferry West Ashley Library
9 a.m. - 8 p.m.
Phone: (843) 805-6892
Village Library
9 a.m. - 6 p.m.
Phone: (843) 884-9741
Keith Summey North Charleston Library
9 a.m. – 8 p.m.
Phone: (843) 744-2489
Mobile Library
9 a.m. - 5 p.m.
Phone: (843) 805-6909
Today's Hours
West Ashley Library
9 a.m. – 7 p.m.
Phone: (843) 766-6635
Main Library
9 a.m. - 8 p.m.
Phone: (843) 805-6930
Folly Beach Library
Closed for renovations
Phone: (843) 588-2001
John L. Dart Library
9 a.m. – 7 p.m.
Phone: (843) 722-7550
St. Paul's/Hollywood Library
9 a.m. - 8 p.m.
Phone: (843) 889-3300
Mt. Pleasant Library
9 a.m. – 8 p.m.
Phone: (843) 849-6161
Dorchester Road Library
9 a.m. - 8 p.m.
Phone: (843) 552-6466
Edgar Allan Poe/Sullivan's Island Library
9 a.m. - 6 p.m.
Phone: (843) 883-3914
John's Island Library
9 a.m. – 8 p.m.
Phone: (843) 559-1945
McClellanville Library
Closed for renovations
Phone: (843) 887-3699
Edisto Library
9 a.m. - 6 p.m.
Phone: (843) 869-2355
Wando Mount Pleasant Library
9 a.m. - 8 p.m.
Phone: (843) 805-6888
Otranto Road Library
9 a.m. - 8 p.m.
Phone: (843) 572-4094
Hurd/St. Andrews Library
9 a.m. - 8 p.m.
Phone: (843) 766-2546
Baxter-Patrick James Island
9 p.m. - 8 p.m.
Phone: (843) 795-6679
Bees Ferry West Ashley Library
9 a.m. - 8 p.m.
Phone: (843) 805-6892
Village Library
9 a.m. - 6 p.m.
Phone: (843) 884-9741
Keith Summey North Charleston Library
9 a.m. – 8 p.m.
Phone: (843) 744-2489
Mobile Library
9 a.m. - 5 p.m.
Phone: (843) 805-6909
Patron Login
menu
Item request has been placed!
×
Item request cannot be made.
×
Processing Request
Comparison of chimeric antigen receptor-T cell-mediated cytotoxicity assays with suspension tumor cells using plate-based image cytometry method.
Item request has been placed!
×
Item request cannot be made.
×
Processing Request
- Author(s): Sun YJ;Sun YJ; Chen YC; Chen YC; Hua WK; Hua WK; Wu SC; Wu SC; Chan LL; Chan LL
- Source:
Cytometry. Part A : the journal of the International Society for Analytical Cytology [Cytometry A] 2023 Jan; Vol. 103 (1), pp. 27-38. Date of Electronic Publication: 2022 Aug 03.- Publication Type:
Journal Article- Language:
English - Source:
- Additional Information
- Source: Publisher: Wiley-Liss Country of Publication: United States NLM ID: 101235694 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1552-4930 (Electronic) Linking ISSN: 15524922 NLM ISO Abbreviation: Cytometry A Subsets: MEDLINE
- Publication Information: Original Publication: Hoboken, N.J. : Wiley-Liss, c2002-
- Subject Terms:
- Abstract: In the recent decade, chimeric antigen receptor (CAR)-T cell therapy has revolutionized strategies for cancer treatments due to its highly effective clinical efficacy and response for B cell malignancies. The success of CAR-T cell therapy has stimulated the increase in the research and development of various CAR constructs to target different tumor types. Therefore, a robust and efficient in vitro potency assay is needed to quickly identify potential CAR gene design from a library of construct candidates. Image cytometry methodologies have been utilized for various CAR-T cell-mediated cytotoxicity assay using different fluorescent labeling methods, mainly due to their ease-of-use, ability to capture cell images for verification, and higher throughput performance. In this work, we employed the Celigo Image Cytometer to evaluate and compare two CAR-T cell-mediated cytotoxicity assays using GFP-expressing or fluorescent dye-labeled myeloma and plasmacytoma cells. The GFP-based method demonstrated higher sensitivity in detecting CAR-T cell-mediated cytotoxicity when compared to the CMFDA/DAPI viability method. We have established the criteria and considerations for the selection of cytotoxicity assays that are fit-for-purpose to ensure the results produced are meaningful for the specific testing conditions.
(© 2022 International Society for Advancement of Cytometry.) - References: Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, et al. Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer. 2015;136:E359-86.
Albinger N, Hartmann J, Ullrich E. Current status and perspective of CAR-T and CAR-NK cell therapy trials in Germany. Gene Ther. 2021;28:513-27.
Thallinger C, Füreder T, Preusser M, Heller G, Müllauer L, Höller C, et al. Review of cancer treatment with immune checkpoint inhibitors. Wien Klin Wochenschr. 2018;130:85-91.
Sterner RC, Sterner RM. CAR-T cell therapy: current limitations and potential strategies. Blood Cancer J. 2021;11:69.
Zheng P-P, Kros JM, Li J. Approved CAR T cell therapies: ice bucket challenges on glaring safety risks and long-term impacts. Drug Discov Today. 2018;23:1175-82.
Han D, Xu Z, Zhuang Y, Ye Z, Qian Q. Current Progress in CAR-T cell therapy for hematological malignancies. J Cancer. 2021;12:326-34.
Huang W, Li J, Liao MZ, Liu SN, Yu J, Jing J, et al. Clinical pharmacology perspectives for adoptive cell therapies in oncology. Clin Pharmacol Ther. 2021;0:1-14.
Cockroft A, Wilson A. Comparability: what we can learn from the review of advanced therapy medicinal products. Regen Med. 2021;16:655-67.
Martin T, Usmani SZ, Berdeja JG, Agha M, Cohen AD, Hari P, et al. Ciltacabtagene autoleucel, an anti-B-cell maturation antigen chimeric antigen receptor T-cell therapy, for relapsed/refractory multiple myeloma: CARTITUDE-1 2-year follow-up. J Clin Oncol. 2022;JCO2200842.
Korzeniewski C, Callewaert DM. An enzyme-release assay for natural cytotoxicity. J Immunol Methods. 1983;64:313-20.
Miller RG, Dunkley M. Quantitative analysis of the 51Cr release cytotoxicity assay for cytotoxic lymphocytes. Cell Immunol. 1974;14:284-302.
Wang XM, Terasaki PI, Rankin GW Jr, Chia D, Zhong HP, Hardy S. A new microcellular cytotoxicity test based on calcein AM release. Hum Immunol. 1993;37:264-70.
Somanchi SS, McCulley KJ, Somanchi A, Chan LL, Lee DA. A novel method for assessment of natural killer cell cytotoxicity using image cytometry. PLoS One. 2015;10:1-15.
McCulley KJ, Somanchi SS. Assessment of natural killer cell cytotoxicity using image cytometry method. Methods Mol Biol. 2016;1441:107-16.
Chan LL-Y, Wucherpfennig KW, Andrade LF. Visualization and quantification of NK cell-mediated cytotoxicity over extended time periods by image cytometry. J Immunol Methods. 2019;469:47-51.
Maldini CR, Love AC, Tosh KW, Chan LL-Y, Gayout K, Smith T, et al. Characterization of CAR T cell expansion and cytotoxic potential during ex vivo manufacturing using image-based cytometry. J Immunol Methods. 2020;484-485:112830. https://doi.org/10.1016/j.jim.2020.112830.
Wang Y, Chan LL-Y, Grimaud M, Fayed A, Zhu Q, Marasco WA. High-throughput image cytometry detection method for CAR-T transduction, cell proliferation, and cytotoxicity assays. Cytometry A. 2020;99(7):689-97. https://doi.org/10.1002/cyto.a.24267.
Mazor Y, Sachsenmeier KF, Yang C, Hansen A, Filderman J, Mulgrew K, et al. Enhanced tumor-targeting selectivity by modulating bispecific antibody binding affinity and format valence. Sci Rep. 2017;7:40098.
Borrok MJ, Mody N, Lu X, Kuhn ML, Wu H, Dall'Acqua WF, et al. An “Fc-silenced” IgG1 format with extended half-life designed for improved stability. J Pharm Sci. 2017;106:1008-17.
Fantini M, David JM, Saric O, Dubeykovskiy A, Cui Y, Mavroukakis SA, et al. Preclinical characterization of a novel monoclonal antibody NEO-201 for the treatment of human carcinomas. Front Immunol. 2018;8:1-14.
Cherradi S, Ayrolles-Torro A, Vezzo-Vié N, Gueguinou N, Denis V, Combes E, et al. Canterel-Thouennon L, Mollevi C and others. Antibody targeting of claudin-1 as a potential colorectal cancer therapy. J Exp Clin Cancer Res. 2017;36:1-13.
Gonzalez-Exposito R, Semiannikova M, Griffiths B, Khan K, Barber LJ, Woolston A, et al. CEA expression heterogeneity and plasticity confer resistance to the CEA-targeting bispecific immunotherapy antibody cibisatamab (CEA-TCB) in patient-derived colorectal cancer organoids. J Immunother Cancer. 2019;7:1-14.
Grote S, Chan KC-H, Baden C, Bösmüller H, Sulyok M, Frauenfeld L, et al. CD276 as a novel CAR NK-92 therapeutic target for neuroblastoma. Adv Cell Gene Ther. 2020;4:e105. - Contributed Indexing: Keywords: CAR-T cell-mediated cytotoxicity; Celigo; chimeric antigen receptor; immuno-oncology; plate-based image cytometry
- Accession Number: 0 (Receptors, Chimeric Antigen)
- Publication Date: Date Created: 20220723 Date Completed: 20230119 Latest Revision: 20230205
- Publication Date: 20240105
- Accession Number: 10.1002/cyto.a.24673
- Accession Number: 35869932
- Source:
Contact CCPL
Copyright 2022 Charleston County Public Library Powered By EBSCO Stacks 3.3.0 [350.3] | Staff Login
No Comments.