Cannabis Use Associates With Reduced Proviral Burden and Inflammatory Cytokine in Tissues From Men With Clade C HIV-1 on Suppressive Antiretroviral Therapy.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
  • Additional Information
    • Source:
      Publisher: Oxford University Press Country of Publication: United States NLM ID: 0413675 Publication Model: Print Cited Medium: Internet ISSN: 1537-6613 (Electronic) Linking ISSN: 00221899 NLM ISO Abbreviation: J Infect Dis Subsets: MEDLINE
    • Publication Information:
      Publication: Jan. 2011- : Oxford : Oxford University Press
      Original Publication: 1904-2010 : Chicago, IL : University of Chicago Press
    • Subject Terms:
    • Abstract:
      Background: Human immunodeficiency virus 1 (HIV-1) tissue reservoirs remain the main obstacle against an HIV cure. Limited information exists regarding cannabis's effects on HIV-1 infections in vivo, and the impact of cannabis use on HIV-1 parenchymal tissue reservoirs is unexplored.
      Methods: To investigate whether cannabis use alters HIV-1 tissue reservoirs, we systematically collected 21 postmortem brain and peripheral tissues from 20 men with subtype C HIV-1 and with suppressed viral load enrolled in Zambia, 10 of whom tested positive for cannabis use. The tissue distribution and copies of subtype C HIV-1 LTR, gag, env DNA and RNA, and the relative mRNA levels of cytokines IL-1β, IL-6, IL-10, and TGF-β1 were quantified using PCR-based approaches. Utilizing generalized linear mixed models we compared persons with HIV-1 and suppressed viral load, with and without cannabis use.
      Results: The odds of tissues harboring HIV-1 DNA and the viral DNA copies in those tissues were significantly lower in persons using cannabis. Moreover, the transcription levels of proinflammatory cytokines IL-1β and IL-6 in lymphoid tissues of persons using cannabis were also significantly lower.
      Conclusions: Our findings suggested that cannabis use is associated with reduced sizes and inflammatory cytokine expression of subtype C HIV-1 reservoirs in men with suppressed viral load.
      Competing Interests: Potential conflicts of interest. All authors: No reported conflicts. All authors have submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest. Conflicts that the editors consider relevant to the content of the manuscript have been disclosed.
      (© The Author(s) 2024. Published by Oxford University Press on behalf of Infectious Diseases Society of America. All rights reserved. For permissions, please e-mail: [email protected].)
    • References:
      Med Hypotheses. 2019 Dec;133:109371. (PMID: 31465975)
      J Virus Erad. 2021 May 17;7(2):100043. (PMID: 34136266)
      Int J Drug Policy. 2017 Apr;42:63-70. (PMID: 28336000)
      Chem Biol Interact. 2021 Feb 25;336:109398. (PMID: 33503444)
      Nat Microbiol. 2019 Apr;4(4):633-644. (PMID: 30718846)
      J Infect Dis. 2021 Jul 2;224(1):92-100. (PMID: 33216132)
      J Virol. 2016 Sep 29;90(20):8968-83. (PMID: 27466426)
      J Clin Invest. 2000 Oct;106(7):839-45. (PMID: 11018071)
      BMC Pediatr. 2021 Jan 11;21(1):31. (PMID: 33430827)
      AIDS Behav. 2020 Apr;24(4):1275-1280. (PMID: 30778810)
      Lancet. 2019 May 4;393(10183):1804-1805. (PMID: 31057166)
      AIDS Res Hum Retroviruses. 2014 Dec;30(12):1216-25. (PMID: 25113915)
      Retrovirology. 2022 May 31;19(1):10. (PMID: 35642061)
      J Clin Invest. 2020 Apr 1;130(4):1699-1712. (PMID: 31910162)
      Nat Med. 2016 Sep;22(9):1043-9. (PMID: 27500724)
      Am J Drug Alcohol Abuse. 2017 Jan;43(1):103-110. (PMID: 27398989)
      J Virol. 2020 Jan 17;94(3):. (PMID: 31694954)
      Nat Commun. 2023 Jul 13;14(1):4186. (PMID: 37443365)
      PLoS One. 2018 Jul 24;13(7):e0201325. (PMID: 30040863)
      Bull Narc. 1982 Jul-Dec;34(3-4):45-60. (PMID: 6985027)
      Addiction. 2023 Jul;118(7):1201-1215. (PMID: 36807954)
      PLoS Pathog. 2015 Sep 24;11(9):e1005153. (PMID: 26402858)
      Neurol Neuroimmunol Neuroinflamm. 2020 Jun 17;7(5):. (PMID: 32554630)
      Clin Infect Dis. 2020 Jan 1;70(1):140-143. (PMID: 31074488)
      J Virol. 2022 Jul 13;96(13):e0012222. (PMID: 35674431)
      Cannabis Cannabinoid Res. 2021 Aug;6(4):288-299. (PMID: 33998855)
      Nat Commun. 2019 Jun 21;10(1):2737. (PMID: 31227699)
      JCI Insight. 2020 Jul 23;5(14):. (PMID: 32544096)
      Drug Alcohol Rev. 2015 Mar;34(2):135-40. (PMID: 25389027)
      PLoS Pathog. 2022 Sep 8;18(9):e1010845. (PMID: 36074794)
      AIDS. 2016 Oct 23;30(16):2545-2550. (PMID: 27428744)
      PLoS Pathog. 2016 Mar 03;12(3):e1005472. (PMID: 26938995)
      Adv Exp Med Biol. 2021;1264:1-13. (PMID: 33332000)
      JCI Insight. 2022 Oct 24;7(20):. (PMID: 36278485)
      Clin Infect Dis. 2018 Jun 1;66(12):1872-1882. (PMID: 29471387)
      AIDS Behav. 2017 Jul;21(7):1996-2004. (PMID: 28213820)
      Ital J Pediatr. 2013 Feb 15;39:13. (PMID: 23415178)
    • Grant Information:
      U54 CA221204 United States CA NCI NIH HHS; TWO10354 Fogarty International D43; R01DA044920 United States NH NIH HHS
    • Contributed Indexing:
      Keywords: cannabis use; inflammatory cytokines; proviral DNA; subtype C HIV-1 tissue reservoirs
    • Publication Date:
      Date Created: 20240120 Date Completed: 20240515 Latest Revision: 20240517
    • Publication Date:
    • Accession Number:
    • Accession Number:
    • Accession Number: