Frequency of HBsAg variants in occult hepatitis B virus infected patients and detection by ARCHITECT HBsAg quantitative.

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  • Author(s): He C;He C; Liu Y; Liu Y; Jiang X; Jiang X; Xu Z; Xu Z; Xiang Z; Xiang Z; Lu Z; Lu Z
  • Source:
    Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2024 May 03; Vol. 14, pp. 1368473. Date of Electronic Publication: 2024 May 03 (Print Publication: 2024).
  • Publication Type:
    Journal Article
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  • Additional Information
    • Source:
      Publisher: Frontiers Media SA Country of Publication: Switzerland NLM ID: 101585359 Publication Model: eCollection Cited Medium: Internet ISSN: 2235-2988 (Electronic) Linking ISSN: 22352988 NLM ISO Abbreviation: Front Cell Infect Microbiol Subsets: MEDLINE
    • Publication Information:
      Original Publication: Lausanne : Frontiers Media SA
    • Subject Terms:
    • Abstract:
      Objective: To analyze the amino acid substitution caused by mutations in the major hydrophilic region (MHR) of the S-region genes in the serum samples of occult hepatitis B virus infection (OBI), and to explore the reasons for the missed detection of HBsAg.
      Method: The full-length gene of the S-region in hepatitis B virus(HBV) in the chronic hepatitis B virus(CHB)(10 samples) and OBI groups(42 samples) was amplified using a lab-developed, two-round PCR amplification technology. The PCR amplification products were sequenced/clone sequenced, and the nucleotide sequences of the S-region gene in HBV were compared to the respective genotype consensus sequence.
      Results: Only 20 of the 42 samples in the OBI group had the S-region genes successfully amplified, with the lowest HBV DNA load of 20.1IU/ml. As S-region genes in HBV, 68 cloned strains were sequenced. In the OBI and CHB groups MHR region, with a mutation rate of 3.21% (155/4828) and 0.70% (5/710). The genetic mutation rate was significantly higher in the OBI group than in the CHB group (P<0.05). The common mutation types in the MHR region were: I126T, L162R, K122E, C124R, and C147Y.Mutations at s122, s126, and s162 were associated with subgenotypes, most of which being C genotypes. The high-frequency mutation sites L162R and K122E found in this study have not been reported in previous literature.
      Conclusion: The results of this study confirmed that MHR mutations can cause the missed detection of HBsAg, giving rise to OBI.
      Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
      (Copyright © 2024 He, Liu, Jiang, Xu, Xiang and Lu.)
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    • Contributed Indexing:
      Keywords: S region mutation; major hydrophilic region; missed detection; occult hepatitis B virus infection; α determinant
    • Publication Date:
      Date Created: 20240520 Date Completed: 20240520 Latest Revision: 20240521
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