Potential for high dynamic range Sedia Limiting Antigen antibody assay to support viral load monitoring during antiretroviral therapy.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
  • Author(s): Sempa JB;Sempa JB;Sempa JB; Welte A; Welte A
  • Source:
    PloS one [PLoS One] 2024 Jun 06; Vol. 19 (6), pp. e0303393. Date of Electronic Publication: 2024 Jun 06 (Print Publication: 2024).
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
    • Publication Information:
      Original Publication: San Francisco, CA : Public Library of Science
    • Subject Terms:
    • Abstract:
      Introduction: Viral Load (VL) monitoring is a crucial component of patient care during antiretroviral therapy (ART) but is not routinely available in many resource-constrained settings, where millions of patients will require care for decades to come. We hypothesise a serologic 'recent infection' test (Sedia LAg assay) which has a high dynamic range for detecting antigen-driven antibody response can provide informative proxies for VL trajectories.
      Methods: A retrospective study where we analysed data linked via specimens in a well-described repository for recent infection test benchmarking (CEPHIA collaboration). Patient panels were comprised of 1) observations straddling ART start; 2) observations from a period of stable viral suppression; 3) observations straddling rebound after a period of viral suppression. We analysed an individual's Sedia LAg ELISA normalised optical density (ODn) trends within these categories. Using groups 2) and 3) we evaluated the specificity and sensitivity of a proposed proxy for "the latest observation is at a time of VL rebound"; proxy was defined as follows: we estimated patient-specific mean-previous-ODn for all observations with at least two preceding virally suppressed observations. We considered various thresholds to define both "VL suppression" and "ODn uptick".
      Results: In regression analysis by category: 1) ODn gradients are statistically significantly negative just after ART-start (p = 0.010); 2) During periods of stable viral suppression, ODn tended to decline, but not statistically significantly, for a range of clinically meaningful "VL suppression" thresholds; 3) comparing ODn values just before, versus at, "VL rebound", ODn changes were statistically significantly increasing at rebound (p = 0.001). In the analysis comparing groups 2) and 3), at a Z score threshold of 0.8, the proposed proxy for a first viral rebound had an observed specificity and sensitivity both close to 90%.
      Conclusion: The high dynamic range of serological tests previously investigated for defining 'recent infection' has potential, as demonstrated using the Sedia LAg ELISA, to provide meaningful information about the success of ART, during treatment initiation, at times of stable suppression, and to flag possible viral rebound. It should be investigated how this can be combined with patient management workflows and (clinical and) other data, to provide efficiencies in long-term monitoring viral control in resource-limited settings.
      Competing Interests: The authors have declared that no competing interests exist.
      (Copyright: © 2024 Sempa, Welte. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
    • References:
      MMWR Morb Mortal Wkly Rep. 2016 Dec 02;65(47):1332-1335. (PMID: 27906910)
      PLoS One. 2019 Jul 26;14(7):e0220345. (PMID: 31348809)
      AIDS. 2014 Oct 23;28(16):2439-49. (PMID: 25144218)
      J Infect Dis. 2017 Jul 1;216(1):72-81. (PMID: 28498985)
      J Acquir Immune Defic Syndr. 2007 Oct 1;46(2):145-50. (PMID: 17589369)
    • Accession Number:
      0 (Anti-Retroviral Agents)
    • Publication Date:
      Date Created: 20240606 Date Completed: 20240606 Latest Revision: 20240608
    • Publication Date:
      20240608
    • Accession Number:
      PMC11156293
    • Accession Number:
      10.1371/journal.pone.0303393
    • Accession Number:
      38843247