PlGF mediates neutrophil elastase-induced airway epithelial cell apoptosis and emphysema.

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  • Additional Information
    • Publication Year:
      2014
    • Author-Supplied Keywords:
      Apoptosis
      Chronic pulmonary obstructive disease
      Emphysema
      Neutrophil elastase
      Placenta growth factor
    • NAICS/Industry Codes:
      NAICS/Industry Codes 112990 All Other Animal Production
    • Abstract:
      Background Chronic pulmonary obstructive disease (COPD) has become the fourth leading cause of death worldwide. Cigarette smoking induces neutrophil elastase (NE) and contributes to COPD, but the detailed mechanisms involved are not fully established. In an animal model of pulmonary emphysema, there are increased expressions of placenta growth factor (PlGF) and lung epithelial (LE) cell apoptosis. This study hypothesized that excessive NE may up-regulate PlGF and that PlGF-induced LE apoptosis mediates the pathogenesis of pulmonary emphysema. Methods Human bronchial epithelial cells, BEAS-2B, and primary mouse type II alveolar epithelial cells were treated with NE. The PlGF promoter activity was examined by luciferase activity assay, while PlGF expression and secretion were evaluated by RT-PCR, Western blotting, and ELISA. Both cell lines were treated with PlGF to evaluate its effects and the downstream signaling pathways leading to LE cell apoptosis. PlGF knockout and wild-type mice were instilled with NE to determine the roles of PlGF and its downstream molecules in NEpromoted mice pulmonary apoptosis and emphysema phenotype. Results The transcriptional factor, early growth response gene-1, was involved in the NE-promoted PlGF promoter activity, and the expression and secretion of PlGF mRNA and protein in LE cells. PlGF-induced LE cell apoptosis and NE-induced mice pulmonary apoptosis and emphysema were mediated by the downstream c-Jun N-terminal kinase (JNK) and protein kinase C (PKC)δ signaling pathways. Conclusion The NE-PlGF-JNK/PKCδ pathway contributes to the pathogenesis of LE cell apoptosis and emphysema. PlGF and its downstream signaling molecules may be potential therapeutic targets for COPD. [ABSTRACT FROM AUTHOR]
    • Abstract:
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    • ISSN:
      14659921
    • Accession Number:
      101777627
  • Citations
    • ABNT:
      HSIN-HAN HOU et al. PlGF mediates neutrophil elastase-induced airway epithelial cell apoptosis and emphysema. Respiratory Research, [s. l.], v. 15, n. 9, p. 55, 2014. Disponível em: . Acesso em: 17 ago. 2019.
    • AMA:
      Hsin-Han Hou, Shih-Lung Cheng, Kuei-Pin Chung, et al. PlGF mediates neutrophil elastase-induced airway epithelial cell apoptosis and emphysema. Respiratory Research. 2014;15(9):55. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=101777627. Accessed August 17, 2019.
    • APA:
      Hsin-Han Hou, Shih-Lung Cheng, Kuei-Pin Chung, Shu-Chen Wei, Po-Nien Tsao, Hsuan-Hsuan Lu, … Chong-Jen Yu. (2014). PlGF mediates neutrophil elastase-induced airway epithelial cell apoptosis and emphysema. Respiratory Research, 15(9), 55. Retrieved from http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=101777627
    • Chicago/Turabian: Author-Date:
      Hsin-Han Hou, Shih-Lung Cheng, Kuei-Pin Chung, Shu-Chen Wei, Po-Nien Tsao, Hsuan-Hsuan Lu, Hao-Chien Wang, and Chong-Jen Yu. 2014. “PlGF Mediates Neutrophil Elastase-Induced Airway Epithelial Cell Apoptosis and Emphysema.” Respiratory Research 15 (9): 55. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=101777627.
    • Harvard:
      Hsin-Han Hou et al. (2014) ‘PlGF mediates neutrophil elastase-induced airway epithelial cell apoptosis and emphysema’, Respiratory Research, 15(9), p. 55. Available at: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=101777627 (Accessed: 17 August 2019).
    • Harvard: Australian:
      Hsin-Han Hou, Shih-Lung Cheng, Kuei-Pin Chung, Shu-Chen Wei, Po-Nien Tsao, Hsuan-Hsuan Lu, Hao-Chien Wang & Chong-Jen Yu 2014, ‘PlGF mediates neutrophil elastase-induced airway epithelial cell apoptosis and emphysema’, Respiratory Research, vol. 15, no. 9, p. 55, viewed 17 August 2019, .
    • MLA:
      Hsin-Han Hou, et al. “PlGF Mediates Neutrophil Elastase-Induced Airway Epithelial Cell Apoptosis and Emphysema.” Respiratory Research, vol. 15, no. 9, Sept. 2014, p. 55. EBSCOhost, search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=101777627.
    • Chicago/Turabian: Humanities:
      Hsin-Han Hou, Shih-Lung Cheng, Kuei-Pin Chung, Shu-Chen Wei, Po-Nien Tsao, Hsuan-Hsuan Lu, Hao-Chien Wang, and Chong-Jen Yu. “PlGF Mediates Neutrophil Elastase-Induced Airway Epithelial Cell Apoptosis and Emphysema.” Respiratory Research 15, no. 9 (September 2014): 55. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=101777627.
    • Vancouver/ICMJE:
      Hsin-Han Hou, Shih-Lung Cheng, Kuei-Pin Chung, Shu-Chen Wei, Po-Nien Tsao, Hsuan-Hsuan Lu, et al. PlGF mediates neutrophil elastase-induced airway epithelial cell apoptosis and emphysema. Respiratory Research [Internet]. 2014 Sep [cited 2019 Aug 17];15(9):55. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=101777627