Magnetic resonance spectroscopy and imaging on fresh human brain tumor biopsies at microscopic resolution.

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  • Additional Information
    • Publication Year:
      2015
    • Author-Supplied Keywords:
      Biopsy
      Fresh tissue
      Glioblastoma
      High-resolution magic angle spinning spectroscopy (HR-MAS)
      Human brain tumor
      Magnetic resonance imaging
      Magnetic resonance microscopy
      Magnetic resonance spectroscopy
    • NAICS/Industry Codes:
      NAICS/Industry Codes 621512 Diagnostic Imaging Centers
    • Abstract:
      The metabolic composition and concentration knowledge provided by magnetic resonance spectroscopy (MRS) liquid and high-resolution magic angle spinning spectroscopy (HR-MAS) has a relevant impact in clinical practice during magnetic resonance imaging (MRI) monitoring of human tumors. In addition, the combination of morphological and chemical information by MRI and MRS has been particularly useful for diagnosis and prognosis of tumor evolution. MRI spatial resolution reachable in human beings is limited for safety reasons and the demanding necessary conditions are only applicable on experimental model animals. Nevertheless, MRS and MRI can be performed on human biopsies at high spatial resolution, enough to allow a direct correlation between the chemical information and the histological features observed in such biopsies. Although HR-MAS is nowadays a well-established technique for spectroscopic analysis of tumor biopsies, with this approach just a mean metabolic profile of the whole sample can be obtained and thus the high histological heterogeneity of some important tumors is mostly neglected. The value of metabolic HR-MAS data strongly depends on a wide statistical analysis and usually the microanatomical rationale for the correlation between histology and spectroscopy is lost. We present here a different approach for the combined use of MRI and MRS on fresh human brain tumor biopsies with native contrast. This approach has been designed to achieve high spatial (18 × 18 × 50 μm) and spectral (0.031 μL) resolution in order to obtain as much spatially detailed morphological and metabolical information as possible without any previous treatment that can alter the sample. The preservation of native tissue conditions can provide information that can be translated to in vivo studies and additionally opens the possibility of performing other techniques to obtain complementary information from the same sample. [ABSTRACT FROM AUTHOR]
    • Abstract:
      Copyright of Analytical & Bioanalytical Chemistry is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
    • ISSN:
      16182642
    • Accession Number:
      109078073
  • Citations
    • ABNT:
      MARTÍNEZ-BISBAL, M. et al. Magnetic resonance spectroscopy and imaging on fresh human brain tumor biopsies at microscopic resolution. Analytical & Bioanalytical Chemistry, [s. l.], v. 407, n. 22, p. 6771, 2015. Disponível em: . Acesso em: 24 ago. 2019.
    • AMA:
      Martínez-Bisbal M, Martínez-Granados B, Rovira V, Celda B, Esteve V. Magnetic resonance spectroscopy and imaging on fresh human brain tumor biopsies at microscopic resolution. Analytical & Bioanalytical Chemistry. 2015;407(22):6771. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=109078073. Accessed August 24, 2019.
    • APA:
      Martínez-Bisbal, M., Martínez-Granados, B., Rovira, V., Celda, B., & Esteve, V. (2015). Magnetic resonance spectroscopy and imaging on fresh human brain tumor biopsies at microscopic resolution. Analytical & Bioanalytical Chemistry, 407(22), 6771. Retrieved from http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=109078073
    • Chicago/Turabian: Author-Date:
      Martínez-Bisbal, M., Beatriz Martínez-Granados, Vicente Rovira, Bernardo Celda, and Vicent Esteve. 2015. “Magnetic Resonance Spectroscopy and Imaging on Fresh Human Brain Tumor Biopsies at Microscopic Resolution.” Analytical & Bioanalytical Chemistry 407 (22): 6771. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=109078073.
    • Harvard:
      Martínez-Bisbal, M. et al. (2015) ‘Magnetic resonance spectroscopy and imaging on fresh human brain tumor biopsies at microscopic resolution’, Analytical & Bioanalytical Chemistry, 407(22), p. 6771. Available at: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=109078073 (Accessed: 24 August 2019).
    • Harvard: Australian:
      Martínez-Bisbal, M, Martínez-Granados, B, Rovira, V, Celda, B & Esteve, V 2015, ‘Magnetic resonance spectroscopy and imaging on fresh human brain tumor biopsies at microscopic resolution’, Analytical & Bioanalytical Chemistry, vol. 407, no. 22, p. 6771, viewed 24 August 2019, .
    • MLA:
      Martínez-Bisbal, M., et al. “Magnetic Resonance Spectroscopy and Imaging on Fresh Human Brain Tumor Biopsies at Microscopic Resolution.” Analytical & Bioanalytical Chemistry, vol. 407, no. 22, Sept. 2015, p. 6771. EBSCOhost, search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=109078073.
    • Chicago/Turabian: Humanities:
      Martínez-Bisbal, M., Beatriz Martínez-Granados, Vicente Rovira, Bernardo Celda, and Vicent Esteve. “Magnetic Resonance Spectroscopy and Imaging on Fresh Human Brain Tumor Biopsies at Microscopic Resolution.” Analytical & Bioanalytical Chemistry 407, no. 22 (September 2015): 6771. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=109078073.
    • Vancouver/ICMJE:
      Martínez-Bisbal M, Martínez-Granados B, Rovira V, Celda B, Esteve V. Magnetic resonance spectroscopy and imaging on fresh human brain tumor biopsies at microscopic resolution. Analytical & Bioanalytical Chemistry [Internet]. 2015 Sep [cited 2019 Aug 24];407(22):6771. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=109078073