Immunosuppressive protocols with tacrolimus after cryopreserved aortal allotransplantation in rats.

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  • Additional Information
    • Publication Year:
      2018
    • Author-Supplied Keywords:
      Anatomy
      Antibodies
      Aorta
      Biochemistry
      Biology and life sciences
      Blood
      Blood vessels
      Body fluids
      Cardiovascular anatomy
      Clinical immunology
      Clinical medicine
      Diagnostic medicine
      Drugs
      Immune physiology
      Immune suppression
      Immune system proteins
      Immunology
      Immunosuppressives
      Major histocompatibility complex
      Medicine and health sciences
      Pathology and laboratory medicine
      Pharmacology
      Physiology
      Proteins
      Research Article
      Signs and symptoms
      Transplant rejection
      Transplantation immunology
    • NAICS/Industry Codes:
      NAICS/Industry Codes 112990 All Other Animal Production
    • Abstract:
      Objectives and design: The aim of our study was to simulate in rats all aspects and techniques used in our new clinical program of cryopreserved alloarterial transplantation and investigate the influence of two immunosuppressive protocols with tacrolimus on acute rejection of these allografts. Materials and methods: Cryopreserved abdominal aortic grafts were transplanted between Brown-Norway and Lewis rats. Tacrolimus (0.2 mg/kg daily) was administered from day 1 to day 30 (TAC1) or from day 7 to day 30 (TAC7), respectively. No immunosuppressed isogeneic (ISO) and allogeneic (ALO) rats combination served as control. Aortal wall infiltration by immunocompetent cells (MHC II+ cells of recipient origin) was studied on day 30 after transplantation. Flow cytometry was used for the analysis of day 30 sera for the presence of donor specific anti-MHC class I and II antibodies. Results: The aortal allografts in both immunosuppressed groups showed regular morphology of aortal wall with no depositions of immunoglobulin G on day 30. The adventitial infiltration of non-immunosuppressed aortal allografts by MHC class II positive cells of recipient origin was significantly higher (ALO 20.7±6.7 cells, P<0.001) compared to both immunosuppressed groups (TAC1 5.9±5.5 cells, TAC7 6.1±5.1 cells). Day 30 sera from the allogeneic non-immunosuppressed animals decreased significantly the binding of fluorescence-labelled MHC class I (46.9±19.4%) and class II (65.8±11.9%) antibody to donors spleen cells compared with day 30 sera from both immunosuppressed groups (TAC1, anti-MHC class I 102.4±4.2%, p < 0.001, anti-MHC class II 102.6±6.0%), (TAC7, anti-MHC class I 79.9±3.3%, p < 0.001, anti-MHC class II 80.9±2.7%). Conclusion: Both immunosuppressed protocols with tacrolimus (administration from day 1 or from day 7 following transplantation) were able to suppress acute cell- and antibody-mediated rejection of cryopreserved abdominal aortic allografts processed in accordance with our new standardized clinical protocol. [ABSTRACT FROM AUTHOR]
    • Abstract:
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    • ISSN:
      19326203
    • Accession Number:
      131158341
  • Citations
    • ABNT:
      SPUNDA, R. et al. Immunosuppressive protocols with tacrolimus after cryopreserved aortal allotransplantation in rats. PLoS ONE, [s. l.], v. 13, n. 8, p. 1, 2018. Disponível em: . Acesso em: 20 out. 2019.
    • AMA:
      Spunda R, Hruby J, Mericka P, et al. Immunosuppressive protocols with tacrolimus after cryopreserved aortal allotransplantation in rats. PLoS ONE. 2018;13(8):1. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=131158341. Accessed October 20, 2019.
    • APA:
      Spunda, R., Hruby, J., Mericka, P., Mlcek, M., Pecha, O., Splith, K., … Spacek, M. (2018). Immunosuppressive protocols with tacrolimus after cryopreserved aortal allotransplantation in rats. PLoS ONE, 13(8), 1. Retrieved from http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=131158341
    • Chicago/Turabian: Author-Date:
      Spunda, Rudolf, Jan Hruby, Pavel Mericka, Mikulas Mlcek, Ondrej Pecha, Kathrin Splith, Moritz Schmelzle, et al. 2018. “Immunosuppressive Protocols with Tacrolimus after Cryopreserved Aortal Allotransplantation in Rats.” PLoS ONE 13 (8): 1. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=131158341.
    • Harvard:
      Spunda, R. et al. (2018) ‘Immunosuppressive protocols with tacrolimus after cryopreserved aortal allotransplantation in rats’, PLoS ONE, 13(8), p. 1. Available at: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=131158341 (Accessed: 20 October 2019).
    • Harvard: Australian:
      Spunda, R, Hruby, J, Mericka, P, Mlcek, M, Pecha, O, Splith, K, Schmelzle, M, Krenzien, F, Lindner, J, Matia, I & Spacek, M 2018, ‘Immunosuppressive protocols with tacrolimus after cryopreserved aortal allotransplantation in rats’, PLoS ONE, vol. 13, no. 8, p. 1, viewed 20 October 2019, .
    • MLA:
      Spunda, Rudolf, et al. “Immunosuppressive Protocols with Tacrolimus after Cryopreserved Aortal Allotransplantation in Rats.” PLoS ONE, vol. 13, no. 8, Aug. 2018, p. 1. EBSCOhost, search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=131158341.
    • Chicago/Turabian: Humanities:
      Spunda, Rudolf, Jan Hruby, Pavel Mericka, Mikulas Mlcek, Ondrej Pecha, Kathrin Splith, Moritz Schmelzle, et al. “Immunosuppressive Protocols with Tacrolimus after Cryopreserved Aortal Allotransplantation in Rats.” PLoS ONE 13, no. 8 (August 9, 2018): 1. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=131158341.
    • Vancouver/ICMJE:
      Spunda R, Hruby J, Mericka P, Mlcek M, Pecha O, Splith K, et al. Immunosuppressive protocols with tacrolimus after cryopreserved aortal allotransplantation in rats. PLoS ONE [Internet]. 2018 Aug 9 [cited 2019 Oct 20];13(8):1. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=131158341