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Short Tandem Repeat (STR) Profiles of Commonly Used Human Ocular Surface Cell Lines.
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- Author(s): McDermott, Alison M; Baidouri, Hasna; Woodward, Ashley M; Kam, Wendy R; Liu, Yang; Chen, Xiaomin; Ziemanski, Jillian F; Vistisen, Kerry; Hazlett, Linda D; Nichols, Kelly K; Argüeso, Pablo; Sullivan, David A
- Source:
Current Eye Research; Sep2018, Vol. 43 Issue 9, p1097-1101, 5p- Subject Terms:
- Source:
- Additional Information
- Abstract: Purpose: The purpose of this study is to establish the short tandem repeat (STR) profiles of several human cell lines commonly used in ocular surface research. Materials and Methods: Independently DNA was extracted from multiple passages of three human corneal epithelial cell lines, two human conjunctival epithelial cell lines and one meibomian gland cell line, from different laboratories actively involved in ocular surface research. The samples were then subjected to STR analysis on a fee-for-service basis in an academic setting and the data compared against that in available databases. Results: The STR profiles for the human corneal epithelial cells were different among the three cell lines studied and for each line the profiles were identical across the samples provided by three laboratories. Profiles for the human conjunctival epithelial cells were different among the two cell lines studied. Profiles for the meibomian gland cell line were identical across the samples provided by three laboratories. No samples were contaminated by elements of other cell lines such as HeLa. Conclusions: This comprehensive study provides verification of STR profiles for commonly used human ocular surface cell lines that can now be used as a reference by others in the field to authenticate the cell lines in use in their own laboratories. [ABSTRACT FROM AUTHOR]
- Abstract: Copyright of Current Eye Research is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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