Three-dimensional culture of chicken primordial germ cells (cPGCs) in defined media containing the functional polymer FP003.

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  • Additional Information
    • Publication Year:
      2018
    • Author-Supplied Keywords:
      Agriculture
      Amniotes
      Animals
      Biochemistry
      Biological cultures
      Biology and life sciences
      Birds
      Cell cultures
      Chemistry
      Chickens
      Cytophotometry
      Eukaryota
      Flow cytometry
      Fowl
      Gamefowl
      Livestock
      Macromolecules
      Materials by structure
      Materials physics
      Materials science
      Organisms
      Physical sciences
      Physics
      Polymer chemistry
      Polymers
      Poultry
      Proteins
      Recombinant proteins
      Research and analysis methods
      Research Article
      Sedimentation
      Spectrophotometry
      Spectrum analysis techniques
      Vertebrates
    • NAICS/Industry Codes:
      NAICS/Industry Codes 112999 All other miscellaneous animal production
      112310 Chicken Egg Production
      112340 Poultry Hatcheries
      311615 Poultry Processing
    • Abstract:
      Scalable production of avian cell lines exhibits a valuable potential on therapeutic application by producing recombinant proteins and as the substrate for virus growth due to the special glycosylation occurs in avian species. Chicken primordial germ cells (cPGCs), a germinal pluripotent avian cell type, present the ability of self-renewal, an anchorage-independent cell growth and the ability to be genetically modified. This cell type could be an interesting bioreactor system for industrial purposes. This study sought to establish an expandable culture system with defined components for three-dimensional (3D) culture of cPGCs. cPGCs were cultured in medium supplemented with the functional polymer FP003. Viscoelasticity was low in this medium but cPGCs did not sediment in culture and efficiencies of space and nutrient utilization were thus enhanced and consequently their expansion was improved. The total number of cPGCs increased by 17-fold after 1 week of culture in 3D-FAot medium, an aseric defined medium containing FP003 polymer, FGF2 and Activin A as growth factors and Ovotransferrin as protein. Moreover, cPGC cell lines stably expressed the germline-specific reporter VASA:tdTOMATO, as well as other markers of cPGCs, for more than 1 month upon culture in 3D-FAot medium, indicating that the characteristics of these cells are maintained. In summary, this novel 3D culture system can be used to efficiently expand cPGCs in suspension without mechanical stirring, which is available for long-term culture and no loss of cellular properties was found. This system provides a platform for large-scale culture of cPGCs. [ABSTRACT FROM AUTHOR]
    • Abstract:
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    • ISSN:
      19326203
    • Accession Number:
      131914011
  • Citations
    • ABNT:
      CHEN, Y.-C. et al. Three-dimensional culture of chicken primordial germ cells (cPGCs) in defined media containing the functional polymer FP003. PLoS ONE, [s. l.], v. 13, n. 9, p. 1, 2018. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=131914011. Acesso em: 22 jan. 2020.
    • AMA:
      Chen Y-C, Chang W-C, Lin S-P, et al. Three-dimensional culture of chicken primordial germ cells (cPGCs) in defined media containing the functional polymer FP003. PLoS ONE. 2018;13(9):1. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=131914011. Accessed January 22, 2020.
    • APA:
      Chen, Y.-C., Chang, W.-C., Lin, S.-P., Minami, M., Jean, C., Hayashi, H., … Pain, B. (2018). Three-dimensional culture of chicken primordial germ cells (cPGCs) in defined media containing the functional polymer FP003. PLoS ONE, 13(9), 1. Retrieved from http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=131914011
    • Chicago/Turabian: Author-Date:
      Chen, Yi-Chen, Wei-Che Chang, Shau-Ping Lin, Masataka Minami, Christian Jean, Hisato Hayashi, Sylvie Rival-Gervier, Tatsuro Kanaki, Shinn-Chih Wu, and Bertrand Pain. 2018. “Three-Dimensional Culture of Chicken Primordial Germ Cells (CPGCs) in Defined Media Containing the Functional Polymer FP003.” PLoS ONE 13 (9): 1. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=131914011.
    • Harvard:
      Chen, Y.-C. et al. (2018) ‘Three-dimensional culture of chicken primordial germ cells (cPGCs) in defined media containing the functional polymer FP003’, PLoS ONE, 13(9), p. 1. Available at: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=131914011 (Accessed: 22 January 2020).
    • Harvard: Australian:
      Chen, Y-C, Chang, W-C, Lin, S-P, Minami, M, Jean, C, Hayashi, H, Rival-Gervier, S, Kanaki, T, Wu, S-C & Pain, B 2018, ‘Three-dimensional culture of chicken primordial germ cells (cPGCs) in defined media containing the functional polymer FP003’, PLoS ONE, vol. 13, no. 9, p. 1, viewed 22 January 2020, .
    • MLA:
      Chen, Yi-Chen, et al. “Three-Dimensional Culture of Chicken Primordial Germ Cells (CPGCs) in Defined Media Containing the Functional Polymer FP003.” PLoS ONE, vol. 13, no. 9, Sept. 2018, p. 1. EBSCOhost, search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=131914011.
    • Chicago/Turabian: Humanities:
      Chen, Yi-Chen, Wei-Che Chang, Shau-Ping Lin, Masataka Minami, Christian Jean, Hisato Hayashi, Sylvie Rival-Gervier, Tatsuro Kanaki, Shinn-Chih Wu, and Bertrand Pain. “Three-Dimensional Culture of Chicken Primordial Germ Cells (CPGCs) in Defined Media Containing the Functional Polymer FP003.” PLoS ONE 13, no. 9 (September 21, 2018): 1. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=131914011.
    • Vancouver/ICMJE:
      Chen Y-C, Chang W-C, Lin S-P, Minami M, Jean C, Hayashi H, et al. Three-dimensional culture of chicken primordial germ cells (cPGCs) in defined media containing the functional polymer FP003. PLoS ONE [Internet]. 2018 Sep 21 [cited 2020 Jan 22];13(9):1. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=131914011