High frequency of benzimidazole resistance alleles in trichostrongyloids from Austrian sheep flocks in an alpine transhumance management system.

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  • Additional Information
    • Publication Year:
      2020
    • Subject Terms:
    • Author-Supplied Keywords:
      Anthelmintic resistance
      Nematode
      Single nucleotide polymorphism
      Trichostrongyloids, pyrosequencing
    • NAICS/Industry Codes:
      NAICS/Industry Codes 112410 Sheep Farming
    • Abstract:
      Background: Infections of small ruminants with trichostrongyloid nematodes often result in reduced productivity and may be detrimental to the host. Anthelmintic resistance (AR) against most anthelmintic drug classes is now widespread amongst the trichostrongyloids. Baseline establishment, followed by regular monitoring of the level of AR, is necessary for farmers and veterinarians to make informed decisions about parasite management. The detection of single nucleotide polymorphisms (SNPs) is a sensitive method to detect AR against benzimidazoles (BZs), one of the most widely used anthelmintic classes. Alpine transhumance constitutes a special type of pasturing of sheep from many different farms, the aim of this study was to investigate the prevalence of benzimidazole resistance alleles in this particular management system. Results: Sixteen sheep flocks in Styria and Salzburg in Austria were examined by pyrosequencing for SNPs at codons 167, 198 and 200 of the isotype-1 β-tubulin gene. The frequency of the resistance-associated exchange F200Y was 87–100% for H. contortus, 77–100% for T. colubriformis and < 5–66% for T. circumcincta. Additionally, the F167Y polymorphism was detected in T. colubriformis from two farms at a frequency of 19 and 23% respectively. Conclusions: The high resistance allele frequency in H. contortus and T. colubriformis in the examined sheep population urgently calls for the development of new treatment strategies to sustainably control trichostrongyloid infections for this kind of pasturing, since the frequent mixing of flocks during the alpine summer grazing must be considered an important risk factor for the spread of resistant nematodes to a large number of farms. [ABSTRACT FROM AUTHOR]
    • Abstract:
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    • ISSN:
      17466148
    • Accession Number:
      143152573
  • Citations
    • ABNT:
      HINNEY, B. et al. High frequency of benzimidazole resistance alleles in trichostrongyloids from Austrian sheep flocks in an alpine transhumance management system. BMC Veterinary Research, [s. l.], v. 16, n. 1, p. 1, 2020. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=143152573. Acesso em: 3 jul. 2020.
    • AMA:
      Hinney B, Schoiswohl J, Melville L, et al. High frequency of benzimidazole resistance alleles in trichostrongyloids from Austrian sheep flocks in an alpine transhumance management system. BMC Veterinary Research. 2020;16(1):1. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=143152573. Accessed July 3, 2020.
    • AMA11:
      Hinney B, Schoiswohl J, Melville L, et al. High frequency of benzimidazole resistance alleles in trichostrongyloids from Austrian sheep flocks in an alpine transhumance management system. BMC Veterinary Research. 2020;16(1):1. Accessed July 3, 2020. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=143152573
    • APA:
      Hinney, B., Schoiswohl, J., Melville, L., Ameen, V. J., Wille-Piazzai, W., Bauer, K., Joachim, A., Krücken, J., Skuce, P. J., & Krametter-Frötscher, R. (2020). High frequency of benzimidazole resistance alleles in trichostrongyloids from Austrian sheep flocks in an alpine transhumance management system. BMC Veterinary Research, 16(1), 1.
    • Chicago/Turabian: Author-Date:
      Hinney, Barbara, Julia Schoiswohl, Lynsey Melville, Vahel J. Ameen, Walpurga Wille-Piazzai, Karl Bauer, Anja Joachim, Jürgen Krücken, Philip J. Skuce, and Reinhild Krametter-Frötscher. 2020. “High Frequency of Benzimidazole Resistance Alleles in Trichostrongyloids from Austrian Sheep Flocks in an Alpine Transhumance Management System.” BMC Veterinary Research 16 (1): 1. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=143152573.
    • Harvard:
      Hinney, B. et al. (2020) ‘High frequency of benzimidazole resistance alleles in trichostrongyloids from Austrian sheep flocks in an alpine transhumance management system’, BMC Veterinary Research, 16(1), p. 1. Available at: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=143152573 (Accessed: 3 July 2020).
    • Harvard: Australian:
      Hinney, B, Schoiswohl, J, Melville, L, Ameen, VJ, Wille-Piazzai, W, Bauer, K, Joachim, A, Krücken, J, Skuce, PJ & Krametter-Frötscher, R 2020, ‘High frequency of benzimidazole resistance alleles in trichostrongyloids from Austrian sheep flocks in an alpine transhumance management system’, BMC Veterinary Research, vol. 16, no. 1, p. 1, viewed 3 July 2020, .
    • MLA:
      Hinney, Barbara, et al. “High Frequency of Benzimidazole Resistance Alleles in Trichostrongyloids from Austrian Sheep Flocks in an Alpine Transhumance Management System.” BMC Veterinary Research, vol. 16, no. 1, May 2020, p. 1. EBSCOhost, search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=143152573.
    • Chicago/Turabian: Humanities:
      Hinney, Barbara, Julia Schoiswohl, Lynsey Melville, Vahel J. Ameen, Walpurga Wille-Piazzai, Karl Bauer, Anja Joachim, Jürgen Krücken, Philip J. Skuce, and Reinhild Krametter-Frötscher. “High Frequency of Benzimidazole Resistance Alleles in Trichostrongyloids from Austrian Sheep Flocks in an Alpine Transhumance Management System.” BMC Veterinary Research 16, no. 1 (May 11, 2020): 1. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=143152573.
    • Vancouver/ICMJE:
      Hinney B, Schoiswohl J, Melville L, Ameen VJ, Wille-Piazzai W, Bauer K, et al. High frequency of benzimidazole resistance alleles in trichostrongyloids from Austrian sheep flocks in an alpine transhumance management system. BMC Veterinary Research [Internet]. 2020 May 11 [cited 2020 Jul 3];16(1):1. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=143152573