Akt inhibition upregulates FasL, downregulates c-FLIPs and induces caspase-8-dependent cell death in Jurkat T lymphocytes.

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  • Additional Information
    • Publication Year:
      2005
    • Author-Supplied Keywords:
      Akt
      apoptosis
      FasL
    • Abstract:
      In T lymphocytes, the role of Akt in regulating Fas/Fas ligand (FasL)-mediated apoptotic signaling and death is not clearly understood. In this study, we observed that inhibition of Akt causes enhanced expression of FasL mRNA and protein and increased death-inducing signaling complex (DISC) formation with Fas-associated death domain (FADD) and procaspase-8 recruitment. Also, caspase-8 was activated at the DISC with accompanying decrease in c-FLIPs expression. FasL neutralizing antibody significantly decreased apoptotic death in the Akt-inhibited T cells. Additionally, Akt inhibition-induced Fas signaling was observed to link to the mitochondrial pathway via Bid cleavage. Further, inhibition of caspase-8 activity effectively blocked the loss of mitochondrial membrane potential and DNA fragmentation, suggesting that DISC formation and subsequent caspase-8 activation are critical initiating events in Akt inhibition-induced apoptotic death in T lymphocytes. These data demonstrate yet another important survival function governed by Akt kinase in T lymphocytes, which involves the regulation of FasL expression and consequent apoptotic signaling.Cell Death and Differentiation (2005) 12, 233-242. doi:10.1038/sj.cdd.4401549 Published online 21 January 2005 [ABSTRACT FROM AUTHOR]
    • Abstract:
      Copyright of Cell Death & Differentiation is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
    • ISSN:
      13509047
    • Accession Number:
      16113646
  • Citations
    • ABNT:
      URIARTE, S. M. et al. Akt inhibition upregulates FasL, downregulates c-FLIPs and induces caspase-8-dependent cell death in Jurkat T lymphocytes. Cell Death & Differentiation, [s. l.], v. 12, n. 3, p. 233, 2005. Disponível em: . Acesso em: 24 ago. 2019.
    • AMA:
      Uriarte SM, Joshi-Barve S, Song Z, et al. Akt inhibition upregulates FasL, downregulates c-FLIPs and induces caspase-8-dependent cell death in Jurkat T lymphocytes. Cell Death & Differentiation. 2005;12(3):233. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=16113646. Accessed August 24, 2019.
    • APA:
      Uriarte, S. M., Joshi-Barve, S., Song, Z., Sahoo, R., Gobejishvili, L., Jala, V. R., … Barve, S. (2005). Akt inhibition upregulates FasL, downregulates c-FLIPs and induces caspase-8-dependent cell death in Jurkat T lymphocytes. Cell Death & Differentiation, 12(3), 233. Retrieved from http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=16113646
    • Chicago/Turabian: Author-Date:
      Uriarte, S.M., S. Joshi-Barve, Z. Song, R. Sahoo, L. Gobejishvili, V.R. Jala, B. Haribabu, C. McClain, and S. Barve. 2005. “Akt Inhibition Upregulates FasL, Downregulates c-FLIPs and Induces Caspase-8-Dependent Cell Death in Jurkat T Lymphocytes.” Cell Death & Differentiation 12 (3): 233. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=16113646.
    • Harvard:
      Uriarte, S. M. et al. (2005) ‘Akt inhibition upregulates FasL, downregulates c-FLIPs and induces caspase-8-dependent cell death in Jurkat T lymphocytes’, Cell Death & Differentiation, 12(3), p. 233. Available at: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=16113646 (Accessed: 24 August 2019).
    • Harvard: Australian:
      Uriarte, SM, Joshi-Barve, S, Song, Z, Sahoo, R, Gobejishvili, L, Jala, VR, Haribabu, B, McClain, C & Barve, S 2005, ‘Akt inhibition upregulates FasL, downregulates c-FLIPs and induces caspase-8-dependent cell death in Jurkat T lymphocytes’, Cell Death & Differentiation, vol. 12, no. 3, p. 233, viewed 24 August 2019, .
    • MLA:
      Uriarte, S. M., et al. “Akt Inhibition Upregulates FasL, Downregulates c-FLIPs and Induces Caspase-8-Dependent Cell Death in Jurkat T Lymphocytes.” Cell Death & Differentiation, vol. 12, no. 3, Mar. 2005, p. 233. EBSCOhost, search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=16113646.
    • Chicago/Turabian: Humanities:
      Uriarte, S.M., S. Joshi-Barve, Z. Song, R. Sahoo, L. Gobejishvili, V.R. Jala, B. Haribabu, C. McClain, and S. Barve. “Akt Inhibition Upregulates FasL, Downregulates c-FLIPs and Induces Caspase-8-Dependent Cell Death in Jurkat T Lymphocytes.” Cell Death & Differentiation 12, no. 3 (March 2005): 233. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=16113646.
    • Vancouver/ICMJE:
      Uriarte SM, Joshi-Barve S, Song Z, Sahoo R, Gobejishvili L, Jala VR, et al. Akt inhibition upregulates FasL, downregulates c-FLIPs and induces caspase-8-dependent cell death in Jurkat T lymphocytes. Cell Death & Differentiation [Internet]. 2005 Mar [cited 2019 Aug 24];12(3):233. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=16113646