Nonviral human beta defensin-3 expression in a bioengineered human skin tissue: A therapeutic alternative for infected wounds.

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  • Additional Information
    • Publication Year:
      2012
    • NAICS/Industry Codes:
      NAICS/Industry Codes 541711 Research and Development in Biotechnology
      112990 All Other Animal Production
      111421 Nursery and Tree Production
    • Abstract:
      The innate immune system differentially regulates the expression of host defense peptides to combat infection during wound healing. We enhanced the expression of a host defense peptide, human beta defensin-3 ( hBD-3), in keratinocytes to generate a three-dimensional biologic dressing to improve healing of infected wounds. The NIKS human keratinocyte cell line was stably transfected ex vivo with a construct containing an epidermis-specific promoter driving hBD-3 ( NIKShBD-3) using nonviral methods. Levels of hBD-3 mRNA and protein in three-dimensional skin tissue produced from NIKShBD-3 were determined using quantitative polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. Tissue architecture was characterized by hematoxylin and eosin staining and by indirect immunofluorescence using proliferation and keratinocyte differentiation markers. Antimicrobial activity was assessed using an in vitro bacterial growth assay and in vivo using a murine burn infection model. Three-dimensional full thickness skin tissues containing epidermal NIKShBD-3 or control NIKS possessed histologic features of interfollicular epidermis and exhibited normal tissue growth and differentiation. NIKShBD-3 tissue contained approximately fivefold more hBD-3 protein than tissue containing unmodified control NIKS. In vitro studies showed that NIKShBD-3 tissue produced a significant reduction in the growth of Staphylococcus aureus multiple peptide resistance factor ( mprF) compared with control tissue. In an in vivo infected murine burn model, NIKShBD-3 tissue resulted in a 90% reduction in bacterial growth. These results demonstrate that sustained delivery of hBD-3 by a bioengineered skin tissue results in a therapeutically relevant reduction in growth of a S. aureus strain in an animal model of infected third-degree burn wounds. [ABSTRACT FROM AUTHOR]
    • Abstract:
      Copyright of Wound Repair & Regeneration is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
    • ISSN:
      10671927
    • Accession Number:
      74981262
  • Citations
    • ABNT:
      GIBSON, A. L. et al. Nonviral human beta defensin-3 expression in a bioengineered human skin tissue: A therapeutic alternative for infected wounds. Wound Repair & Regeneration, [s. l.], v. 20, n. 3, p. 414, 2012. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=74981262. Acesso em: 12 dez. 2019.
    • AMA:
      Gibson AL, Thomas VCL, Centanni JM, et al. Nonviral human beta defensin-3 expression in a bioengineered human skin tissue: A therapeutic alternative for infected wounds. Wound Repair & Regeneration. 2012;20(3):414. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=74981262. Accessed December 12, 2019.
    • APA:
      Gibson, A. L., Thomas, V. C. L., Centanni, J. M., Schlosser, S. J., Johnston, C. E., Van Winkle, K. F., … Allen, H. B. L. (2012). Nonviral human beta defensin-3 expression in a bioengineered human skin tissue: A therapeutic alternative for infected wounds. Wound Repair & Regeneration, 20(3), 414. Retrieved from http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=74981262
    • Chicago/Turabian: Author-Date:
      Gibson, Angela L., Virnig, Christina L. Thomas, John M. Centanni, Sandy J. Schlosser, Colette E. Johnston, Kelly F. Van Winkle, Andrea Szilagyi, Li‐Ke He, Ravi Shankar, and Hoffmann, B. Lynn Allen. 2012. “Nonviral Human Beta Defensin-3 Expression in a Bioengineered Human Skin Tissue: A Therapeutic Alternative for Infected Wounds.” Wound Repair & Regeneration 20 (3): 414. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=74981262.
    • Harvard:
      Gibson, A. L. et al. (2012) ‘Nonviral human beta defensin-3 expression in a bioengineered human skin tissue: A therapeutic alternative for infected wounds’, Wound Repair & Regeneration, 20(3), p. 414. Available at: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=74981262 (Accessed: 12 December 2019).
    • Harvard: Australian:
      Gibson, AL, Thomas, VCL, Centanni, JM, Schlosser, SJ, Johnston, CE, Van Winkle, KF, Szilagyi, A, He, L, Shankar, R & Allen, HBL 2012, ‘Nonviral human beta defensin-3 expression in a bioengineered human skin tissue: A therapeutic alternative for infected wounds’, Wound Repair & Regeneration, vol. 20, no. 3, p. 414, viewed 12 December 2019, .
    • MLA:
      Gibson, Angela L., et al. “Nonviral Human Beta Defensin-3 Expression in a Bioengineered Human Skin Tissue: A Therapeutic Alternative for Infected Wounds.” Wound Repair & Regeneration, vol. 20, no. 3, May 2012, p. 414. EBSCOhost, search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=74981262.
    • Chicago/Turabian: Humanities:
      Gibson, Angela L., Virnig, Christina L. Thomas, John M. Centanni, Sandy J. Schlosser, Colette E. Johnston, Kelly F. Van Winkle, Andrea Szilagyi, Li‐Ke He, Ravi Shankar, and Hoffmann, B. Lynn Allen. “Nonviral Human Beta Defensin-3 Expression in a Bioengineered Human Skin Tissue: A Therapeutic Alternative for Infected Wounds.” Wound Repair & Regeneration 20, no. 3 (May 2012): 414. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=74981262.
    • Vancouver/ICMJE:
      Gibson AL, Thomas VCL, Centanni JM, Schlosser SJ, Johnston CE, Van Winkle KF, et al. Nonviral human beta defensin-3 expression in a bioengineered human skin tissue: A therapeutic alternative for infected wounds. Wound Repair & Regeneration [Internet]. 2012 May [cited 2019 Dec 12];20(3):414. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=74981262