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9 a.m. – 7 p.m.
Phone: (843) 766-6635
Main Library
9 a.m. - 8 p.m.
Phone: (843) 805-6930
Folly Beach Library
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Phone: (843) 588-2001
John L. Dart Library
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St. Paul's/Hollywood Library
9 a.m. - 8 p.m.
Phone: (843) 889-3300
Mt. Pleasant Library
9 a.m. – 8 p.m.
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DNA methylation changes between relapse and remission of minimal change nephrotic syndrome.
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- Author(s): Kobayashi, Yasuko; Aizawa, Akira; Takizawa, Takumi; Yoshizawa, Chikage; Horiguchi, Hiromi; Ikeuchi, Yuka; Kakegawa, Satoko; Watanabe, Toshio; Maruyama, Kenichi; Morikawa, Akihiro; Hatada, Izuho; Arakawa, Hirokazu
- Source:
Pediatric Nephrology; Dec2012, Vol. 27 Issue 12, p2233-2241, 9p, 1 Diagram, 3 Charts, 3 Graphs- Subject Terms:
- Source:
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- Abstract: Background: DNA methylation of gene promoters is associated with transcriptional inactivation. Changes in DNA methylation can lead to differences in gene expression levels and thereby influence disease development. We hypothesized that epigenetics underlies the pathogenesis of minimal change nephrotic syndrome (MCNS). Methods: Genome-wide DNA methylation changes between relapse and remission in monocytes ( n = 6) and naive T helper cells (Th0s) ( n = 4) isolated from patients with MCNS were investigated using the microarray-based integrated analysis of methylation by isochizomers (MIAMI) method. We confirmed the MIAMI results using bisulfite-pyrosequencing analysis. Expression analysis was performed using quantitative real-time PCR. Results: Three gene loci ( GATA2, PBX4, and NYX) were significantly less methylated in Th0s during relapse than in remission, compared to none in monocytes. In addition, the distance distribution from the regression line of all probes in MIAMI was significantly different between monocytes and Th0s. The mRNA levels of the three genes in Th0s were not significantly different between relapse and remission. Conclusions: Our results demonstrate that the change in DNA methylation patterns from remission to relapse in MCNS occurs predominantly in Th0s rather than in monocytes and suggest that epigenetic regulation in Th0s underlies the pathogenesis of MCNS. [ABSTRACT FROM AUTHOR]
- Abstract: Copyright of Pediatric Nephrology is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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