Endogenous interleukin ( IL)-17 A promotes pristane-induced systemic autoimmunity and lupus nephritis induced by pristane.

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    • Abstract:
      Interleukin ( IL)-17 A is increased both in serum and in kidney biopsies from patients with lupus nephritis, but direct evidence of pathogenicity is less well established. Administration of pristane to genetically intact mice results in the production of autoantibodies and proliferative glomerulonephritis, resembling human lupus nephritis. These studies sought to define the role of IL-17 A in experimental lupus induced by pristane administration. Pristane was administered to wild-type ( WT) and IL-17 A−/− mice. Local and systemic immune responses were assessed after 6 days and 8 weeks, and autoimmunity, glomerular inflammation and renal injury were measured at 7 months. IL-17 A production increased significantly 6 days after pristane injection, with innate immune cells, neutrophils ( Ly6 G+) and macrophages ( F4/80+) being the predominant source of IL-17 A. After 8 weeks, while systemic IL-17 A was still readily detected in WT mice, the levels of proinflammatory cytokines, interferon ( IFN)-γ and tumour necrosis factor ( TNF) were diminished in the absence of endogenous IL-17 A. Seven months after pristane treatment humoral autoimmunity was diminished in the absence of IL-17 A, with decreased levels of immunoglobulin ( Ig)G and anti-ds DNA antibodies. Renal inflammation and injury was less in the absence of IL-17 A. Compared to WT mice, glomerular IgG, complement deposition, glomerular CD4+ T cells and intrarenal expression of T helper type 1 ( Th1)-associated proinflammatory mediators were decreased in IL-17 A−/− mice. WT mice developed progressive proteinuria, but functional and histological renal injury was attenuated in the absence of IL-17 A. Therefore, IL-17 A is required for the full development of autoimmunity and lupus nephritis in experimental SLE, and early in the development of autoimmunity, innate immune cells produce IL-17A. [ABSTRACT FROM AUTHOR]
    • Abstract:
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