Imunohistohemijska analiza ekspresije α-aktina glatkih mišića i glijalnog kiselog fibrilarnog proteina u stelatnim ćelijama jetre. (Croatian).

Immunohistochemical analysis of α-SMA and GFAP expression in liver stellate cells. (English)

HEPATITIS B; IMMUNOHISTOCHEMISTRY; BIOMARKERS; BIOPSY; NEUROGLIA

Background/Aim. Liver stellate cells play an important role in hepatic fibrosis, and its progression to cirrhosis. These cells show immunoreactivity with different monoclonal antibodies amongst which the commonest are α-smooth muscle actin (α-SMA) and glial fibrillary acid protein (GFAP). The aim of this study was to analyze stellate cell immunoreactivity for α-SMA and GFAP in tissue sections showing the signs of chronic viral B-hepatitis and compare it with those without histopathological changes. Methods. We included 12 tissue samples showing chronic viral B hepatitis in the different stages of fibrosis and 7 tissue samples showing no histopathological changes. Immunohistochemical staining was performed using the streptavidinbiotin method. Results. There was a regular presence of α-SMA immunoreactivity in tissue sections without histopathological changes in the portal tracts and also in liver parenchyma, while GFAP expression was noted in the periportal cavity. Tissue sections with the signs of chronic viral B hepatitis displayed very strong α-SMA expression in the portal tracts. A statistical analysis showed a positive correlation between the degree of liver fibrosis and α-SMA expression along the fibrous septa, whereas a negative correlation between the degree of liver fibrosis and α-SMA expression was present in the portal zone. Conclusion. This study showed the existence of two different stellate cell subpopulations in liver tissue. Differentiation between them was possible on the basis of SMA/GFAP expression. [ABSTRACT FROM AUTHOR]

Uvod/Cilj. Aktivisane stelatne ćelije igraju glavnu ulogu u procesu fibrogeneze u jetri i u napredovanju ovog procesa do ciroze. Imunoreaktivne su na veliki broj markera, među kojima su najpoznatiji α-aktin glatkih mišića (α-SMA), glijalni kiseli fibrilarni protein (GFAP), dezmin i drugi. Cilj studije je analiza imunoreaktivnosti stelatnih ćelija na α-SMA i GFAP markere u biopsijskim uzorcima bez patoloških promena i uzorcima tkiva jetre bolesnika sa hronič-nim B-virusnim hepatitisom. Metode. U ovoj studiji ispitivali smo 12 biopsijskih uzoraka tkiva jetre obolelih od hroničnog B-virusnog hepatitisa i sedam uzoraka tkiva jetre koji nisu pokazali histopatološke promene. Za imunohistohemijska bojenja koristili smo streptavidin-biotin metod. Rezultati. α-SMA imunoaktivnost u kontrolnoj grupi ispitanika postoji u portnim prostorima i u parenhimu, a GFAP marker u periportnoj zoni jetre, kako zdravih, tako i bolesnih ispitanika. U biopsijskim uzorcima sa hroničnim B-virusnim hepatitisom uočava se izrazita α-SMA imuno-reaktivnost u portnim prostorima. Statistička analiza pokazala je postojanje pozitivne korelacije stepena fibroze i ekspresije SMA markera u zoni vezivnih septuma, a negativna korelacija postoji u zoni portnih prostora. Zaključak. Rezultati ove studije ukazuju na postojanje subpopulacija stelatnih ćelija u tkivu jetre koje karakteriše različita SMA/GFAP imunoreaktivnost. [ABSTRACT FROM AUTHOR]

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