Imunohistohemijska analiza angiogeneze kod invazivnog karcinoma duktusa dojke i korelacija sa kliničko-patološkim faktorima. (Croatian).

Immunohistochemical analysis of angiogenesis in invasive ductal breast carcinoma with correlations to clinicopathological factor. (English)

NEOVASCULARIZATION; TUMORS; BREAST cancer; IMMUNOHISTOCHEMISTRY; HORMONE receptors

Background/Aim. Angiogenesis is the formation of new vessels from preexisting ones. The aim of our study was to determine the relevance of tumor-induced angiogenesis, its correlation with some of the commonly used clinical, pathological factors and the recent biological indicators, and metastatic potential of the tumor in a series of 120 patients with invasive ductal carcinoma of the breast. Methods. The identification of microvessels was performed immunohistochemically with factor VIII-related antigen. The microvessel count (MVC) was assessed at the invasive front of each carcinoma. The cases were divided into high-and low-microvessel density groups according to an average number of microvessels found in the multiple fields of the most vessel-dense part of each tumor. The nuclear immunohistochemical staining for hormone receptors, and the p53, and the membranous staining for c-erbB-2 were evalueted. Results. There were significant correlations between a high MVC and a large tumor size, high histological grade, and c-erbB-2 protein overexpression. There was no association between tumor angiogenesis, as assessed by the MVC, and the hormone receptors status, and the p53 protein expression. In the cases with a high MVC, there was a significant number of tumors with lymph node metastases. Conclusion. Our findings showed that a high MVC might point out an aggressive tumor phenotype. This might help to stratify patients for an appropriate therapy on an individual basis and, thus, offer the possibility of a more effectively tailored treatment program. [ABSTRACT FROM AUTHOR]

Uvod/Cilj. Angiogeneza je proces stvaranja novih krvnih sudova iz postojeće vaskularne mreže. U radu je ispitivan značaj tumorski indukovane angiogeneze kod 120 invazivnih karcinoma duktusa dojke određivanjem korelacije sa standardnim kliničko-patološkim prognostičkim faktorima i faktorima metastatskog potencijala karcinoma dojke, kao i ispitivanjem korelacije angiogeneze sa novijim biomarkerima prognoze i potencijalnim prognostičkim faktorima. Metode. Identifikacija krvnih sudova vršena je imunohistohemijski, uz primarno antitelo faktor VIII. Angiogeneza je procenjivana kvantitativno, brojanjem krvnih sudova na invazivnom rubu infiltrativnog karcinoma duktusa dojke. Na osnovu broja novoformiranih krvnih sudova (BKS), svi slučajevi su podeljeni u grupu sa malim prosečnim BKS i grupu sa velikim BKS. Ekspresija estrogenskih i progesteronskih receptora, p53 i c-erbB-2 proteina određivana je semikvantitativno, nakon imunohistohemijske vizualizacije korišćenjem specifičnih primarnih antitela. Rezultati. Stepen tumorski indukovane angiogeneze je pozitivno korelisao sa veličinom tumora, histološkim gradusom, kao i ekspresijom c-erbB-2 proteina. Stepen ekspresije hormonskih receptora i p53 proteina nije bio u korelaciji sa nivoom tumorski indukovane angiogeneze. U grupi hipervaskularnih tumora statistički značajno su bili brojniji karcinomi dojke sa prisutnim metastazama u limfnim čvorovima. Zaključak. Visok nivo angiogeneze u invazivnom karcinomu duktusa dojke može da ukaže na agresivniji tumorski fenotip. Određivanje stepena angiogeneze uz druge prognostičke i prediktivne biomarkere u karcinomu dojke može da pomogne pri optimalnom izboru terapije prilagođene svakoj bolesnici. [ABSTRACT FROM AUTHOR]

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