Modulation of ACh release by presynaptic muscarinic autoreceptors in the neuromuscular junction of the newborn and adult rat.

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    • Subject Terms:
    • Abstract:
      Abstract We studied the presynaptic muscarinic autoreceptor subtypes controlling ACh release and their relationship with voltage-dependent calcium channels in the neuromuscular synapses of the Levator auris longus muscle from adult (30–40 days) and newborn (3–6 and 15 days postnatal) rats. Using intracellular recording, we studied how several muscarinic antagonists affected the evoked endplate potentials. In some experiments we previously incubated the muscle with calcium channel blockers (nitrendipine, ω-conotoxin-GVIA and ω-Agatoxin-IVA) before determining the muscarinic response. In the adult, the M1 receptor-selective antagonist pirenzepine (10 µm) reduced evoked neurotransmission (≈ 47%). The M2 receptor-selective antagonist methoctramine (1 µm) increased the evoked release (≈ 67%). Both M1- and M2-mediated mechanisms depend on calcium influx via P/Q-type synaptic channels. We found nothing to indicate the presence of M3 (4-DAMP-sensitive) or M4 (tropicamide-sensitive) receptors in the muscles of adult or newborn rats. In the 3–6-day newborn rats, pirenzepine reduced the evoked release (≈ 30%) by a mechanism independent of L-, N- and P/Q-type calcium channels, and the M2 antagonist methoctramine (1 µm) unexpectedly decreased the evoked release (≈ 40%). This methoctramine effect was a P/Q-type calcium-channel-dependent mechanism. However, upon maturation in the first two postnatal weeks, the M2 pathway shifted to perform the calcium-dependent release-inhibitory activity found in the adult. We show that the way in which M1 and M2 muscarinic receptors modulate neurotransmission can differ between the developing and adult rat neuromuscular synapse. [ABSTRACT FROM AUTHOR]
    • Abstract:
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    • ISSN:
      0953-816X
    • Accession Number:
      10.1046/j.1460-9568.2003.02428.x
    • Accession Number:
      8838565
  • Citations
    • ABNT:
      SANTAFÉ, M. M. et al. Modulation of ACh release by presynaptic muscarinic autoreceptors in the neuromuscular junction of the newborn and adult rat. European Journal of Neuroscience, [s. l.], v. 17, n. 1, p. 119–127, 2003. DOI 10.1046/j.1460-9568.2003.02428.x. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=pbh&AN=8838565. Acesso em: 5 jul. 2020.
    • AMA:
      Santafé MM, Salon I, Garcia N, Lanuza MA, Uchitel OD, Tomàs J. Modulation of ACh release by presynaptic muscarinic autoreceptors in the neuromuscular junction of the newborn and adult rat. European Journal of Neuroscience. 2003;17(1):119-127. doi:10.1046/j.1460-9568.2003.02428.x.
    • AMA11:
      Santafé MM, Salon I, Garcia N, Lanuza MA, Uchitel OD, Tomàs J. Modulation of ACh release by presynaptic muscarinic autoreceptors in the neuromuscular junction of the newborn and adult rat. European Journal of Neuroscience. 2003;17(1):119-127. doi:10.1046/j.1460-9568.2003.02428.x
    • APA:
      Santafé, M. M., Salon, I., Garcia, N., Lanuza, M. A., Uchitel, O. D., & Tomàs, J. (2003). Modulation of ACh release by presynaptic muscarinic autoreceptors in the neuromuscular junction of the newborn and adult rat. European Journal of Neuroscience, 17(1), 119–127. https://doi.org/10.1046/j.1460-9568.2003.02428.x
    • Chicago/Turabian: Author-Date:
      Santafé, Manel M., Isabel Salon, Neus Garcia, M. Angel Lanuza, Osvaldo D. Uchitel, and Josep Tomàs. 2003. “Modulation of ACh Release by Presynaptic Muscarinic Autoreceptors in the Neuromuscular Junction of the Newborn and Adult Rat.” European Journal of Neuroscience 17 (1): 119–27. doi:10.1046/j.1460-9568.2003.02428.x.
    • Harvard:
      Santafé, M. M. et al. (2003) ‘Modulation of ACh release by presynaptic muscarinic autoreceptors in the neuromuscular junction of the newborn and adult rat’, European Journal of Neuroscience, 17(1), pp. 119–127. doi: 10.1046/j.1460-9568.2003.02428.x.
    • Harvard: Australian:
      Santafé, MM, Salon, I, Garcia, N, Lanuza, MA, Uchitel, OD & Tomàs, J 2003, ‘Modulation of ACh release by presynaptic muscarinic autoreceptors in the neuromuscular junction of the newborn and adult rat’, European Journal of Neuroscience, vol. 17, no. 1, pp. 119–127, viewed 5 July 2020, .
    • MLA:
      Santafé, Manel M., et al. “Modulation of ACh Release by Presynaptic Muscarinic Autoreceptors in the Neuromuscular Junction of the Newborn and Adult Rat.” European Journal of Neuroscience, vol. 17, no. 1, Jan. 2003, pp. 119–127. EBSCOhost, doi:10.1046/j.1460-9568.2003.02428.x.
    • Chicago/Turabian: Humanities:
      Santafé, Manel M., Isabel Salon, Neus Garcia, M. Angel Lanuza, Osvaldo D. Uchitel, and Josep Tomàs. “Modulation of ACh Release by Presynaptic Muscarinic Autoreceptors in the Neuromuscular Junction of the Newborn and Adult Rat.” European Journal of Neuroscience 17, no. 1 (January 2003): 119–27. doi:10.1046/j.1460-9568.2003.02428.x.
    • Vancouver/ICMJE:
      Santafé MM, Salon I, Garcia N, Lanuza MA, Uchitel OD, Tomàs J. Modulation of ACh release by presynaptic muscarinic autoreceptors in the neuromuscular junction of the newborn and adult rat. European Journal of Neuroscience [Internet]. 2003 Jan [cited 2020 Jul 5];17(1):119–27. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=pbh&AN=8838565