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Academic Journal

Studying RAS Interactions in Live Cells with BRET.

  • Authors : Columbus J; NCI RAS Initiative, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, MD, USA.; Turbyville T

Subjects: Bioluminescence Resonance Energy Transfer Techniques*/Bioluminescence Resonance Energy Transfer Techniques*/Bioluminescence Resonance Energy Transfer Techniques*/methods ; High-Throughput Screening Assays*; Energy Transfer

  • Source: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2797, pp. 253-260.Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029

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A novel BRET-based assay to investigate binding and residence time of unmodified ligands to the human lysosomal ion channel TRPML1 in intact cells.

  • Authors : Cunha MR; Center of Medicinal Chemistry (CQMED), Center for Molecular Biology and Genetic Engineering (CBMEG), University of Campinas, Campinas, Brazil. Electronic address: .; Catta-Preta CMC

Subjects: Bioluminescence Resonance Energy Transfer Techniques*/Bioluminescence Resonance Energy Transfer Techniques*/Bioluminescence Resonance Energy Transfer Techniques*/methods ; Transient Receptor Potential Channels*/Transient Receptor Potential Channels*/Transient Receptor Potential Channels*/metabolism; Humans

  • Source: The Journal of biological chemistry [J Biol Chem] 2023 Jun; Vol. 299 (6), pp. 104807. Date of Electronic Publication: 2023 May 11.Publisher: Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Country of Publication: United States NLM ID: 2985121R Publication

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Highly Sensitive and Selective Detection of Inorganic Phosphates in the Water Environment by Biosensors Based on Bioluminescence Resonance Energy Transfer.

  • Authors : Yu J; State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, Jiangsu 210023, China.; Zhang Y

Subjects: Bioluminescence Resonance Energy Transfer Techniques*/Bioluminescence Resonance Energy Transfer Techniques*/Bioluminescence Resonance Energy Transfer Techniques*/methods ; Biosensing Techniques*/Biosensing Techniques*/Biosensing Techniques*/methods; Phosphates

  • Source: Analytical chemistry [Anal Chem] 2023 Mar 21; Vol. 95 (11), pp. 4904-4913. Date of Electronic Publication: 2023 Mar 09.Publisher: American Chemical Society Country of Publication: United States NLM ID: 0370536 Publication Model: Print-Electronic Cited Medium: Internet

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Academic Journal

Measuring Protein-Protein Interactions in Cells using Nanoluciferase Bioluminescence Resonance Energy Transfer (NanoBRET) Assay.

  • Authors : Szewczyk MM; Structural Genomics Consortium, University of Toronto, Toronto, ON, Canada.; Owens DDG

Subjects: Luminescent Measurements*/Luminescent Measurements*/Luminescent Measurements*/methods ; Bioluminescence Resonance Energy Transfer Techniques*/Bioluminescence Resonance Energy Transfer Techniques*/Bioluminescence Resonance Energy Transfer Techniques*/methods; Reproducibility of Results

  • Source: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2706, pp. 137-148.Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029

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Academic Journal

Application of bioluminescence resonance energy transfer to quantitate cell-surface expression of membrane proteins.

  • Authors : Mattheisen JM; Laboratory of Chemical Biology and Signal Transduction, The Rockefeller University, New York, NY, 10065, USA; Tri-Institutional PhD Program in Chemical Biology, New York, NY, 10065, USA.

Subjects: Membrane Proteins* ; Receptors, G-Protein-Coupled*; Cell Membrane/Cell Membrane/Cell Membrane/metabolism

  • Source: Analytical biochemistry [Anal Biochem] 2024 Jan 01; Vol. 684, pp. 115361. Date of Electronic Publication: 2023 Oct 20.Publisher: Elsevier Country of Publication: United States NLM ID: 0370535 Publication Model: Print-Electronic Cited Medium: Internet ISSN:

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Molecular determinants of ligand efficacy and potency in GPCR signaling.

  • Authors : Heydenreich FM; Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA, USA.; MRC Laboratory of Molecular Biology, Cambridge, UK.

Subjects: Adrenergic beta-2 Receptor Agonists*/Adrenergic beta-2 Receptor Agonists*/Adrenergic beta-2 Receptor Agonists*/chemistry ; Adrenergic beta-2 Receptor Agonists*/Adrenergic beta-2 Receptor Agonists*/Adrenergic beta-2 Receptor Agonists*/pharmacology ; Receptors, Adrenergic, beta-2*/Receptors, Adrenergic, beta-2*/Receptors, Adrenergic, beta-2*/chemistry

  • Source: Science (New York, N.Y.) [Science] 2023 Dec 22; Vol. 382 (6677), pp. eadh1859. Date of Electronic Publication: 2023 Dec 22.Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 0404511 Publication Model: Print-Electronic

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Deciphering downstream receptor signaling.

  • Authors : Filizola M; Department of Pharmacological Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA.; Javitch JA

Subjects: Drug Discovery* ; Receptors, Adrenergic, beta-1*/Receptors, Adrenergic, beta-1*/Receptors, Adrenergic, beta-1*/chemistry ; Adrenergic beta-1 Receptor Agonists*/Adrenergic beta-1 Receptor Agonists*/Adrenergic beta-1 Receptor Agonists*/chemistry

  • Source: Science (New York, N.Y.) [Science] 2023 Dec 22; Vol. 382 (6677), pp. 1357-1358. Date of Electronic Publication: 2023 Dec 21.Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 0404511 Publication Model: Print-Electronic

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Monitoring TRPC7 Conformational Changes by BRET Following GPCR Activation.

  • Authors : Pétigny C; Department of Pharmacology-Physiology, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada.; Institut de Pharmacologie de Sherbrooke, Université de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada.

Subjects: Bioluminescence Resonance Energy Transfer Techniques*/Bioluminescence Resonance Energy Transfer Techniques*/Bioluminescence Resonance Energy Transfer Techniques*/methods ; Biosensing Techniques*/Biosensing Techniques*/Biosensing Techniques*/methods; Animals

  • Source: International journal of molecular sciences [Int J Mol Sci] 2022 Feb 24; Vol. 23 (5). Date of Electronic Publication: 2022 Feb 24.Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067

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Academic Journal

Probing the binding of interleukin-23 to individual receptor components and the IL-23 heteromeric receptor complex in living cells using NanoBRET.

  • Authors : Lay CS; Division of Physiology, Pharmacology and Neuroscience, School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK; Centre of Membrane Proteins and Receptors, Universities of Birmingham and Nottingham, The Midlands, UK

Subjects: Bioluminescence Resonance Energy Transfer Techniques*; Interleukin-23/Interleukin-23/Interleukin-23/*immunology ; Luciferases/Luciferases/Luciferases/*immunology

  • Source: Cell chemical biology [Cell Chem Biol] 2022 Jan 20; Vol. 29 (1), pp. 19-29.e6. Date of Electronic Publication: 2021 May 25.Publisher: Cell Press Country of Publication: United States NLM ID: 101676030 Publication Model: Print-Electronic Cited Medium: Internet ISSN:

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Screening of Protein-Protein Interaction Modulators Using BRET-Based Technology.

  • Authors : Fernández LR; Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Orgánica, Buenos Aires, Argentina.; CONICET-Universidad de Buenos Aires, Unidad de Microanálisis y Métodos Físicos Aplicados a la Química Orgánica (UMYMFOR), Buenos Aires, Argentina.

Subjects: Bioluminescence Resonance Energy Transfer Techniques*/Bioluminescence Resonance Energy Transfer Techniques*/Bioluminescence Resonance Energy Transfer Techniques*/methods ; Trypanosoma cruzi*; Biological Assay

  • Source: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2525, pp. 173-183.Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029

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  • 1-10 of  260 results for ""Bioluminescence Resonance Energy Transfer Techniques""