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[Three-dimensional cell cultures. Applications in basic science and biotechnology].
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- Author(s): Kitel R;Kitel R; Czarnecka J; Czarnecka J; Rusin A; Rusin A
- Source:
Postepy biochemii [Postepy Biochem] 2013; Vol. 59 (3), pp. 305-14.- Publication Type:
English Abstract; Journal Article; Review- Language:
Polish - Source:
- Additional Information
- Transliterated Title: Trójwymiarowe hodowle komórek--zastosowania w badaniach podstawowych i inzynierii tkankowej.
- Source: Publisher: Panstwowe Wydawnictwo Naukowe Country of Publication: Poland NLM ID: 0023525 Publication Model: Print Cited Medium: Print ISSN: 0032-5422 (Print) Linking ISSN: 00325422 NLM ISO Abbreviation: Postepy Biochem Subsets: MEDLINE
- Publication Information: Publication: Warszawa : Panstwowe Wydawnictwo Naukowe
Original Publication: Warszawa. - Subject Terms:
- Abstract: The tissue culture technique is widely used in biochemical and molecular studies, offering accessibility to biological material, high reproducibility of results and high throughput format, comparing with organ cultures. However, traditional, two-dimensional cultures (2D cultures) poorly represent the microenvironment of a tissue, and they are gradually replaced with 3D cultures, that enable formation of intercellular contacts, signaling pathways and gene expression characteristic for tissue in vivo. This paper presents the biology of three-dimensional cultures (spheroids), their applications in basic science and biotechnology and methods of spheroids formation.
- Publication Date: Date Created: 20131225 Date Completed: 20140123 Latest Revision: 20140408
- Publication Date: 20221213
- Accession Number: 24364213
- Transliterated Title:
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