Development of a novel multi-epitope oral DNA vaccine for rabies based on a food-borne microbial vector.

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  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE
    • Publication Information:
      Publication: Amsterdam : Elsevier
      Original Publication: Guildford, Eng., IPC Science and Technology Press.
    • Subject Terms:
      Rabies*/prevention & control ; Vaccines, DNA* ; Rabies Vaccines*/genetics ; Rabies virus*/genetics; Humans ; Animals ; Mice ; Escherichia coli ; Antibodies, Viral ; Epitopes, B-Lymphocyte/genetics
    • Abstract:
      Rabies has been with humans for a long time, and its special transmission route and almost 100 % lethality rate made it once a nightmare for humans. In this study, by predicting the rabies virus glycoprotein outer membrane region and nucleoprotein B-cell antigenic epitopes, the coding sequence of the predicted highly antigenic polypeptide region obtained was assembled using the eukaryotic expression vector pcDNA3.1(-), and then E. coli was used as the delivery vector. The immunogenicity and protective properties of the vaccine were verified by in vivo and in vitro experiments, which demonstrated that the vaccine could produce antibodies in mice and prolong the survival time of mice exposed to the strong virus without any side effects. This study demonstrated that the preparation of an oral rabies DNA vaccine using food-borne microorganisms as a transport vehicle is feasible and could be a new strategy to eradicate rabies starting with wild animals.
      Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have influenced the work reported in this paper.
      (Copyright © 2023. Published by Elsevier B.V.)
    • Contributed Indexing:
      Keywords: DNA vaccine; Microbial vector; Oral vaccine; Rabies
    • Accession Number:
      0 (Vaccines, DNA)
      0 (Antibodies, Viral)
      0 (Rabies Vaccines)
      0 (Epitopes, B-Lymphocyte)
    • Publication Date:
      Date Created: 20231117 Date Completed: 20240110 Latest Revision: 20240110
    • Publication Date:
      20240110
    • Accession Number:
      10.1016/j.ijbiomac.2023.128085
    • Accession Number:
      37977454