Species-specific identification of human hookworms by PCR of the mitochondrial cytochrome oxidase I gene.

Publisher: American Society of Parasitologists [etc.] Country of Publication: United States NLM ID: 7803124 Publication Model: Print Cited Medium: Print ISSN: 0022-3395 (Print) Linking ISSN: 00223395 NLM ISO Abbreviation: J Parasitol Subsets: MEDLINE

Original Publication: Lawrence, Kans. [etc.] American Society of Parasitologists [etc.]

Ancylostoma/*classification ; Ancylostomiasis/*parasitology ; DNA, Helminth/*genetics ; Electron Transport Complex IV/*genetics ; Necator americanus/*classification ; Necatoriasis/*parasitology; Ancylostoma/enzymology ; Ancylostoma/genetics ; Animals ; Base Sequence ; China ; Cricetinae ; DNA, Helminth/chemistry ; DNA, Helminth/isolation & purification ; DNA, Mitochondrial/chemistry ; DNA, Mitochondrial/genetics ; DNA, Mitochondrial/isolation & purification ; Electron Transport Complex IV/chemistry ; Humans ; Mesocricetus ; Molecular Sequence Data ; Necator americanus/enzymology ; Necator americanus/genetics ; Polymerase Chain Reaction/methods ; Sensitivity and Specificity ; Sequence Homology, Nucleic Acid ; Species Specificity

Significant differences in the life histories of the human hookworms Ancylostoma duodenale and Necator americanus necessitate their differentiation for epidemiological studies and the design of control programs. Current methods of identification require time-consuming, labor-intensive techniques. A polymerase chain reaction (PCR)-based method that enables rapid species identification is described. The mitochondrial cytochrome oxidase I genes of both species were sequenced, and species-specific primer sets were designed. The primers were used in PCR to amplify 585-bp fragments of the cytochrome oxidase gene from individual hookworm eggs, larvae, and adults. The technique was also able to identify mixed infections containing equal amounts of eggs from each species. The technique is rapid, technically simple, and sensitive and will permit the accurate identification of human hookworms in epidemiological field studies.

AI-32726 United States AI NIAID NIH HHS; P50 AI-39461 United States AI NIAID NIH HHS

0 (DNA, Helminth)
0 (DNA, Mitochondrial)
EC 1.9.3.1 (Electron Transport Complex IV)

Date Created: 20011107 Date Completed: 20011204 Latest Revision: 20071114

20240513

10.1645/0022-3395(2001)087[1227:SSIOHH]2.0.CO;2

11695411