Identification of a set of genes involved in the formation of the substrate for the incorporation of the unusual "glycolate" chain extension unit in ansamitocin biosynthesis.

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  • Author(s): Carroll BJ;Carroll BJ; Moss SJ; Bai L; Kato Y; Toelzer S; Yu TW; Floss HG
  • Source:
    Journal of the American Chemical Society [J Am Chem Soc] 2002 Apr 24; Vol. 124 (16), pp. 4176-7.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: American Chemical Society Country of Publication: United States NLM ID: 7503056 Publication Model: Print Cited Medium: Print ISSN: 0002-7863 (Print) Linking ISSN: 00027863 NLM ISO Abbreviation: J Am Chem Soc Subsets: MEDLINE
    • Publication Information:
      Publication: Washington, DC : American Chemical Society
      Original Publication: Easton, Pa. [etc.]
    • Subject Terms:
      Genes, Bacterial* ; Multigene Family*; Actinomycetales/*genetics ; Glycolates/*metabolism ; Maytansine/*analogs & derivatives ; Maytansine/*metabolism; Actinomycetales/metabolism ; Multienzyme Complexes/genetics ; Multienzyme Complexes/metabolism
    • Abstract:
      The unusual "glycolate" extender unit at C-9/C-10 of ansamitocin is not derived from 2-hydroxymalonyl-CoA or 2-methoxymalonyl-CoA, as demonstrated by feeding experiments with the corresponding 1-13C-labeled N-acetylcysteamine thioesters but is formed from an acyl carrier protein (ACP)-bound substrate, possibly 2-methoxymalonyl-ACP, elaborated by enzymes encoded by a subcluster of five genes, asm12-17, from the ansamitocin bisosynthetic gene cluster.
    • Grant Information:
      CA 76461 United States CA NCI NIH HHS
    • Accession Number:
      0 (Glycolates)
      0 (Multienzyme Complexes)
      14083FR882 (Maytansine)
      69279-90-9 (ansamitocins)
    • Publication Date:
      Date Created: 20020419 Date Completed: 20020624 Latest Revision: 20190708
    • Publication Date:
      20231215
    • Accession Number:
      10.1021/ja0124764
    • Accession Number:
      11960423