Mutagen specificity and position effects on mutation in T4rII nonsense sites.

Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0400763 Publication Model: Print Cited Medium: Print ISSN: 0027-5107 (Print) Linking ISSN: 00275107 NLM ISO Abbreviation: Mutat Res Subsets: MEDLINE

Original Publication: Amsterdam : Elsevier, [1964-

DNA, Viral* ; Mutation*; Coliphages/*metabolism; 2-Aminopurine/pharmacology ; Alleles ; Base Sequence ; Bromouracil/pharmacology ; Codon ; Genes ; Hydroxylamines/pharmacology ; Methylnitronitrosoguanidine/pharmacology ; Viral Proteins

In 14 sites in the T4rII region, spontaneous and induced interconversions of ochre (UAA) and opal (UGA) alleles, as well as the reversion of the nonsense sites to r+, were studied. The mutagens employed were 2-aminopurine (2AP), 5-bromouracil (5BU), N-methyl-N'-nitro-N-nitrosoguanidine (NTG) and hydroxylamine (HA). With the test system employed, mutagen specificity (i.e., the preferential induction of A: T leads to G: C or G: C leads to A: T mutation at a given site) can be studied. Simultaneously, the response of similar base pairs at various locations in the same or in different nucleotide triplets throughout the rII region, to a given mutagen, can be compared. 2-Aminopurine can induce transitions of both A: T and G: C base pairs at high rates. This mutagen shows no preference for either direction. Furthermore, there is a correlation between the response to 2AP of an A: T pair occupying a given site, and that of a G: C pair occupying the same site. NTG and HA induce G: C leads to A: T transitions almost exclusively. However, there is a correlation between the low rates of A: T leads to G: C transition induced in each of the various sites by these mutagens and those of G: C leads to A: T. 5-Bromouracil induces transitions from G: C to A: T more readily than from A: T to G: C. With 5BU-induced mutation, there is no correlation between the rates of G: C leads to A: T transitions and those of A: T leads to G: C. In UAA sites, all three adenine:thymidine paris respond to 2AP mutagenesis in a similar pattern, In each position in the triplet, response to 2 AP is correlated with that to 5BU. In UGA sites, there are correlations among the spontaneous as well as the 2AP-, HA- and NTG-induced transition rates. 5BU-induced transition rates are usually not correlated with those induced by other mutagens or with the sponatneous ones.

0 (Codon)
0 (DNA, Viral)
0 (Hydroxylamines)
0 (Viral Proteins)
12H3O2UGSF (Methylnitronitrosoguanidine)
452-06-2 (2-Aminopurine)
4HK400G5UO (Bromouracil)

Date Created: 19760101 Date Completed: 19760423 Latest Revision: 20190702

20240513

10.1016/0027-5107(76)90258-x

1250247