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Main Library
2 p.m. – 5 p.m.
Phone: (843) 805-6930
McClellanville Library
Closed
Phone: (843) 887-3699
Folly Beach Library
Closed
Phone: (843) 588-2001
Miss Jane's Building (Edisto Library Temporary Location)
Closed for renovations
Phone: (843) 869-2355
West Ashley Library
Closed
Phone: (843) 766-6635
John L. Dart Library
Closed
Phone: (843) 722-7550
St. Paul's/Hollywood Library
Closed
Phone: (843) 889-3300
Mt. Pleasant Library
Closed
Phone: (843) 849-6161
Dorchester Road Library
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Phone: (843) 552-6466
Edgar Allan Poe/Sullivan's Island Library
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Phone: (843) 883-3914
John's Island Library
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Phone: (843) 559-1945
Wando Mount Pleasant Library
Closed
Phone: (843) 805-6888
Otranto Road Library
Closed
Phone: (843) 572-4094
Hurd/St. Andrews Library
Closed
Phone: (843) 766-2546
Baxter-Patrick James Island
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Phone: (843) 795-6679
Bees Ferry West Ashley Library
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Phone: (843) 805-6892
Village Library
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Phone: (843) 884-9741
Keith Summey North Charleston Library
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Phone: (843) 744-2489
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Establishment of a Pseudovirus Platform for Neuraminidase Inhibiting Antibody Analysis.
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- Author(s): Desheva, Yulia; Petkova, Nadezhda; Losev, Igor; Guzhov, Dmitry; Go, Alexey; Chao, Yu-Chan; Tsai, Chih-Hsuan
- Source:
International Journal of Molecular Sciences; Feb2023, Vol. 24 Issue 3, p2376, 13p- Subject Terms:
- Source:
- Additional Information
- Subject Terms:
- Abstract: Neuraminidase (NA)-based immunity to influenza can be useful for protecting against novel antigenic variants. To develop safe and effective tools to assess NA-based immunity, we generated a baculovirus-based pseudotyped virus, N1-Bac, that expresses the full-length NA of the influenza A/California/07/2009 (H1N1)pdm09 strain. We evaluated the level of NA-inhibiting (NI) antibodies in the paired blood sera of influenza patients by means of an enzyme-linked lectin assay (ELLA) using the influenza virus or N1-Bac. Additionally, we evaluated the level of NA antibodies by means of the enzyme-linked immunosorbent assay (ELISA) with an N1-expressing Sf21 culture. We detected a strong correlation between our results from using the influenza virus and NA-Bac pseudoviruses to detect NI antibodies and a medium-strong correlation between NI antibodies and NA antibodies determined by an N1-cell ELISA, indicating that baculovirus-based platforms can be successfully used to evaluate NI or NA antibodies. Furthermore, animal experiments showed that immunization with N1-Bac protected against infection with a drift variant of the A/H1N1pdm09 influenza virus. Our results demonstrate that recombinant baculovirus can be an effective influenza pseudotype to evaluate influenza serologic immunity and protect against influenza virus infection. [ABSTRACT FROM AUTHOR]
- Abstract: Copyright of International Journal of Molecular Sciences is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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