[Obtaining of isolated enterocytes from the small intestine of cattle fetuses under the control of biochemical indices of cell fractions].

Publisher: Nat︠s︡ionalʹna akademii︠a︡ nauk Ukraïny, Instytut biokhimiï im. O.V. Palladina Country of Publication: Ukraine NLM ID: 101657175 Publication Model: Print Cited Medium: Print NLM ISO Abbreviation: Ukr Biokhim Zh (1999) Subsets: MEDLINE

Original Publication: Kyïv : Nat︠s︡ionalʹna akademii︠a︡ nauk Ukraïny, Instytut biokhimiï im. O.V. Palladina, 1999-2013.

Enterocytes*/cytology ; Enterocytes*/metabolism ; Intestinal Mucosa*/cytology ; Intestinal Mucosa*/embryology ; Intestinal Mucosa*/metabolism ; Intestine, Small*/cytology ; Intestine, Small*/embryology ; Intestine, Small*/metabolism; Animals ; Cattle ; Cell Culture Techniques ; Cell Separation ; Time Factors

The modification of chemical method for obtaining of isolated bovine fetal small intestine enterocytes is experimentally substantiated in the paper. The necessity of time reduction of incubation of small intestine in solution of chelating agents for obtaining the high-cleared fraction of absorptive cells with the low degree of their destruction is proved.

Date Created: 20071123 Date Completed: 20080102 Latest Revision: 20150803

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