Effects of nimodipine administration on small bowel mucosa under conditions of laparotomy and consequent 48-hour starvation in a rat model.

Publisher: Casa Editrice Licinio Cappelli Country of Publication: Italy NLM ID: 0372343 Publication Model: Print Cited Medium: Print ISSN: 0003-469X (Print) Linking ISSN: 0003469X NLM ISO Abbreviation: Ann Ital Chir Subsets: MEDLINE

Original Publication: Bologna : Casa Editrice Licinio Cappelli

Apoptosis* ; Laparotomy* ; Starvation*; Calcium Channel Blockers/*pharmacology ; Intestinal Mucosa/*drug effects ; Intestinal Mucosa/*surgery ; Intestine, Small/*drug effects ; Intestine, Small/*surgery ; Nimodipine/*pharmacology; Animal Experimentation ; Animals ; Calcium Channel Blockers/administration & dosage ; Data Interpretation, Statistical ; Enterocytes/drug effects ; Ileum/surgery ; Immunohistochemistry ; Injections, Intraperitoneal ; Jejunum/surgery ; Male ; Nimodipine/administration & dosage ; Rats ; Rats, Wistar ; Reperfusion Injury/prevention & control ; Time Factors

Background/aims: The combination of starvation and surgical trauma induces disturbances to the intestinal mucosal structure and function, as well as changes in mucosal barrier function in the rat small bowel. The aim of the present study was to evaluate the effects of nimodipine administration, on intestinal mucosal structural changes and enterocyte apoptosis, following laparotomy and subsequent postsurgical starvation (PSS) in the rat.
Methods: Thirty Wistar rats were divided into two experimental groups: A: Control group (n=15), where the animal models underwent laparotomy and consequent 48-hours PSS and B: Nimodipine group (n=15), where the rats underwent laparotomy, followed by intraperitoneal nimodipine administration and consequent 48-hour (h) PSS. Small bowel mucosal structural changes and enterocyte epithelial apoptosis were determined 48 h following laparotomy.
Results: Nimodipine rats (group B) demonstrated a significant decrease in small bowel villous height in jejunum (p=0.016) and ileum (p=0.002). Similarly, crypt depth decreased in jejunum (p<0.001) and ileum (p<0.001). Nimodipine group exhibited significantly higher apoptotic index in ileum compared to control rats (p=0.006).
Conclusion: Nimodipine did not protect the intestinal mucosa from damage caused by surgery and consequent PSS and had obvious damaging effects on intestinal mucosa with derangements to its structure and subsequent mucosal atrophy.

0 (Calcium Channel Blockers)
57WA9QZ5WH (Nimodipine)

Date Created: 20080830 Date Completed: 20081023 Latest Revision: 20191210

20231215

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