Acylglycerol kinase functions as an oncogene and an unfavorable prognostic marker of human gliomas.

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  • Additional Information
    • Source:
      Publisher: W B Saunders Country of Publication: United States NLM ID: 9421547 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-8392 (Electronic) Linking ISSN: 00468177 NLM ISO Abbreviation: Hum Pathol Subsets: MEDLINE
    • Publication Information:
      Publication: Philadelphia, PA : W B Saunders
      Original Publication: Philadelphia, W B. Saunders Co.
    • Subject Terms:
      Oncogenes*; Biomarkers, Tumor/*metabolism ; Brain Neoplasms/*enzymology ; Glioma/*epidemiology ; Phosphotransferases (Alcohol Group Acceptor)/*metabolism; Biomarkers, Tumor/genetics ; Brain Neoplasms/genetics ; Brain Neoplasms/mortality ; Brain Neoplasms/pathology ; Cell Line, Tumor ; Cell Movement ; Cell Proliferation ; Female ; Gene Expression Regulation, Neoplastic ; Glioma/genetics ; Glioma/mortality ; Glioma/pathology ; Humans ; Kaplan-Meier Estimate ; Male ; Middle Aged ; Multivariate Analysis ; Neoplasm Grading ; Neoplasm Invasiveness ; Phosphotransferases (Alcohol Group Acceptor)/genetics ; Proportional Hazards Models ; RNA Interference ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Risk Factors ; Signal Transduction ; Transfection
    • Abstract:
      Acylglycerol kinase (AGK) regulates various cellular processes involved into tumorigenesis and tumor progression. To investigate involvement of AGK in human gliomas, here, quantitative real-time polymerase chain reaction, Western blot, and immunohistochemistry analyses were performed to respectively detect the expression of AGK mRNA and protein in glioma and nonneoplastic brain tissue specimens. Then, the associations of AGK expression with various clinicopathological characteristics and patients' prognosis were statistically evaluated. Moreover, the effects of siRNA-mediated AGK knockdown on glioma cell proliferation, migration, and invasion were respectively assessed via Cell Counting Kit-8 and Transwell assays in vitro. As a result, AGK expression, at both mRNA and protein levels, were markedly up-regulated in glioma tissues compared with nonneoplastic brain tissues (both P < .001). In addition, high AGK expression was significantly associated with the grade of malignancy and poor prognosis in glioma patients (all P < .05). Importantly, Cox regression model of multivariate analysis identified AGK expression as an independent prognostic factor for glioma patients (P = .03). Furthermore, silencing the expression of AGK dramatically suppressed cell proliferation, migration, and invasion of glioma cells in vitro (all P < .05). In conclusion, AGK up-regulation may be involved into glioma development and progression, highlighting its prognostic value for the treatment of patients with this malignancy. Further loss-of-function experiments suggest that AGK might play an important role in the viability and motility of glioma cells, implying its potentials as an attractive therapeutic target for this tumor.
      (Copyright © 2016 Elsevier Inc. All rights reserved.)
    • Contributed Indexing:
      Keywords: Acylglycerol kinase; Cell proliferation; Glioma; Invasion; Migration; Prognosis
    • Accession Number:
      0 (Biomarkers, Tumor)
      0 (RNA, Messenger)
      EC 2.7.1.- (AGK protein, human)
      EC 2.7.1.- (Phosphotransferases (Alcohol Group Acceptor))
    • Publication Date:
      Date Created: 20160831 Date Completed: 20170828 Latest Revision: 20180114
    • Publication Date:
      20231215
    • Accession Number:
      10.1016/j.humpath.2016.07.034
    • Accession Number:
      27574811