The Munich MIDY Pig Biobank - A unique resource for studying organ crosstalk in diabetes.

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  • Additional Information
    • Source:
      Publisher: Elsevier GmbH Country of Publication: Germany NLM ID: 101605730 Publication Model: eCollection Cited Medium: Internet ISSN: 2212-8778 (Electronic) Linking ISSN: 22128778 NLM ISO Abbreviation: Mol Metab Subsets: MEDLINE
    • Publication Information:
      Original Publication: [München] : Elsevier GmbH, 2012-
    • Subject Terms:
      Body Fluids* ; Disease Models, Animal* ; Tissue Banks*; Diabetes Mellitus, Type 2/*genetics ; Insulin/*genetics ; Swine/*genetics; Animals ; Diabetes Mellitus, Type 2/pathology ; Diabetes Mellitus, Type 2/veterinary ; Female ; Germany
    • Abstract:
      Objective: The prevalence of diabetes mellitus and associated complications is steadily increasing. As a resource for studying systemic consequences of chronic insulin insufficiency and hyperglycemia, we established a comprehensive biobank of long-term diabetic INS C94Y transgenic pigs, a model of mutant INS gene-induced diabetes of youth (MIDY), and of wild-type (WT) littermates.
      Methods: Female MIDY pigs (n = 4) were maintained with suboptimal insulin treatment for 2 years, together with female WT littermates (n = 5). Plasma insulin, C-peptide and glucagon levels were regularly determined using specific immunoassays. In addition, clinical chemical, targeted metabolomics, and lipidomics analyses were performed. At age 2 years, all pigs were euthanized, necropsied, and a broad spectrum of tissues was taken by systematic uniform random sampling procedures. Total beta cell volume was determined by stereological methods. A pilot proteome analysis of pancreas, liver, and kidney cortex was performed by label free proteomics.
      Results: MIDY pigs had elevated fasting plasma glucose and fructosamine concentrations, C-peptide levels that decreased with age and were undetectable at 2 years, and an 82% reduced total beta cell volume compared to WT. Plasma glucagon and beta hydroxybutyrate levels of MIDY pigs were chronically elevated, reflecting hallmarks of poorly controlled diabetes in humans. In total, ∼1900 samples of different body fluids (blood, serum, plasma, urine, cerebrospinal fluid, and synovial fluid) as well as ∼17,000 samples from ∼50 different tissues and organs were preserved to facilitate a plethora of morphological and molecular analyses. Principal component analyses of plasma targeted metabolomics and lipidomics data and of proteome profiles from pancreas, liver, and kidney cortex clearly separated MIDY and WT samples.
      Conclusions: The broad spectrum of well-defined biosamples in the Munich MIDY Pig Biobank that will be available to the scientific community provides a unique resource for systematic studies of organ crosstalk in diabetes in a multi-organ, multi-omics dimension.
    • References:
      Diabetes. 2013 May;62(5):1505-11. (PMID: 23274907)
      Theriogenology. 2016 Jul 1;86(1):406-21. (PMID: 27180329)
      Science. 2000 Sep 22;289(5487):2122-5. (PMID: 11000114)
      J Am Coll Cardiol. 2017 Jan 17;69(2):131-143. (PMID: 28081822)
      Cell Metab. 2016 May 10;23(5):770-84. (PMID: 27166942)
      Nat Rev Endocrinol. 2014 Dec;10(12):723-36. (PMID: 25287287)
      Proc Natl Acad Sci U S A. 2012 May 1;109(18):7049-54. (PMID: 22499789)
      Nature. 2008 Mar 27;452(7186):423-8. (PMID: 18344981)
      CMAJ. 2003 Apr 1;168(7):859-66. (PMID: 12668546)
      Sci Rep. 2016 Sep 16;6:33362. (PMID: 27634466)
      Anesthesiology. 2011 Dec;115(6):1363-81. (PMID: 22020140)
      Diabetes. 2010 May;59(5):1228-38. (PMID: 20185813)
      Diabetologia. 2017 Aug;60(8):1541-1549. (PMID: 28480495)
      Anesthesiology. 2008 Dec;109(6):1113-31. (PMID: 19034109)
      Exp Mol Med. 2016 Mar 11;48:e214. (PMID: 26964830)
      Eur J Lipid Sci Technol. 2015 Oct;117(10):1540-1549. (PMID: 26494980)
      PLoS One. 2014 Oct 28;9(10):e110384. (PMID: 25350558)
      Toxicol Pathol. 2016 Apr;44(3):414-20. (PMID: 26883152)
      Am J Physiol Endocrinol Metab. 2007 Dec;293(6):E1687-96. (PMID: 17911348)
      Acta Physiol (Oxf). 2014 Apr;210(4):733-53. (PMID: 24495317)
      Nature. 2015 Mar 26;519(7544):397-8. (PMID: 25810182)
      Mol Aspects Med. 2015 Apr;42:3-18. (PMID: 25542748)
      Sci Rep. 2017 Feb 21;7:42970. (PMID: 28220896)
      Eur J Clin Nutr. 2017 Jul;71(7):896-903. (PMID: 28294170)
      Methods Mol Biol. 2015;1222:37-59. (PMID: 25287337)
      Curr Cardiovasc Risk Rep. 2013 Feb;7(1):73-83. (PMID: 23326608)
      Diabetes. 2015 Mar;64(3):718-32. (PMID: 25713200)
      Physiol Rev. 2013 Jan;93(1):137-88. (PMID: 23303908)
    • Contributed Indexing:
      Keywords: Biobank; CE, cholesterol ester; CPT1, carnitine O-palmitoyltransferase 1; ER, endoplasmic reticulum; FFA, free fatty acids; Hyperglycemia; Insulin insufficiency; MIDY; MIDY, mutant INS gene-induced diabetes of youth; Metabolomics; PC, phosphatidylcholine; PCA, principal component analysis; Pig model; Proteomics; Random systematic sampling; SM, sphingomyelin; Stereology; TAG, triacylglycerol; Transcriptomics; WT, wild-type
    • Accession Number:
      0 (Insulin)
    • Publication Date:
      Date Created: 20170729 Date Completed: 20181212 Latest Revision: 20231112
    • Publication Date:
      20240513
    • Accession Number:
      PMC5518720
    • Accession Number:
      10.1016/j.molmet.2017.06.004
    • Accession Number:
      28752056