Arabidopsis ABCG28 is required for the apical accumulation of reactive oxygen species in growing pollen tubes.

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  • Additional Information
    • Source:
      Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1091-6490 (Electronic) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE
    • Publication Information:
      Original Publication: Washington, DC : National Academy of Sciences
    • Subject Terms:
      Arabidopsis/*metabolism ; Arabidopsis Proteins/*physiology ; Pollen Tube/*metabolism ; Reactive Oxygen Species/*metabolism; Amino Acid Sequence ; Arabidopsis Proteins/chemistry ; Pollen Tube/growth & development ; Protein Conformation ; Sequence Homology, Amino Acid
    • Abstract:
      Tip-focused accumulation of reactive oxygen species (ROS) is tightly associated with pollen tube growth and is thus critical for fertilization. However, it is unclear how tip-growing cells establish such specific ROS localization. Polyamines have been proposed to function in tip growth as precursors of the ROS, hydrogen peroxide. The ABC transporter AtABCG28 may regulate ROS status, as it contains multiple cysteine residues, a characteristic of proteins involved in ROS homeostasis. In this study, we found that AtABCG28 was specifically expressed in the mature pollen grains and pollen tubes. AtABCG28 was localized to secretory vesicles inside the pollen tube that moved toward and fused with the plasma membrane of the pollen tube tip. Knocking out AtABCG28 resulted in defective pollen tube growth, failure to localize polyamine and ROS to the growing pollen tube tip, and complete male sterility, whereas ectopic expression of this gene in root hair could recover ROS accumulation at the tip and improved the growth under high-pH conditions, which normally prevent ROS accumulation and tip growth. Together, these data suggest that AtABCG28 is critical for localizing polyamine and ROS at the growing tip. In addition, this function of AtABCG28 is likely to protect the pollen tube from the cytotoxicity of polyamine and contribute to the delivery of polyamine to the growing tip for incorporation into the expanding cell wall.
      Competing Interests: The authors declare no conflict of interest.
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    • Contributed Indexing:
      Keywords: ABC transporters; AtABCG28; ROS; pollen tube growth; polyamine
    • Accession Number:
      0 (Arabidopsis Proteins)
      0 (Reactive Oxygen Species)
    • Publication Date:
      Date Created: 20190602 Date Completed: 20200406 Latest Revision: 20200408
    • Publication Date:
      20231215
    • Accession Number:
      PMC6589667
    • Accession Number:
      10.1073/pnas.1902010116
    • Accession Number:
      31152136