Tools and methods for circular dichroism spectroscopy of proteins: a tutorial review.

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  • Author(s): Miles AJ;Miles AJ; Janes RW; Wallace BA
  • Source:
    Chemical Society reviews [Chem Soc Rev] 2021 Aug 07; Vol. 50 (15), pp. 8400-8413. Date of Electronic Publication: 2021 Jun 15.
  • Publication Type:
    Journal Article; Review
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Chemical Society Country of Publication: England NLM ID: 0335405 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1460-4744 (Electronic) Linking ISSN: 03060012 NLM ISO Abbreviation: Chem Soc Rev Subsets: MEDLINE
    • Publication Information:
      Publication: Letchworth : Chemical Society
      Original Publication: London, Chemical Society.
    • Subject Terms:
      Circular Dichroism/*instrumentation ; Circular Dichroism/*methods ; Proteins/*chemistry; Biochemistry ; Humans
    • Abstract:
      Circular dichroism (CD) spectroscopy is a widely-used method in biochemistry, structural biology and pharmaceutical chemistry. More than 24 000 papers published in the past decade have included CD characterisations of proteins; many of those studies have also included other complementary chemical, biophysical, and computational chemistry methods. This tutorial review describes the background to the technique of CD spectroscopy and good practice methods for high quality data collection. It specifically focuses on both established and new methods and tools available for experimental design and interpretation, data processing, visualisation, analysis, validation, archiving, and accession, including tools developed to enhance the complementarity of this method with other structural and chemical biology studies.
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    • Grant Information:
      BB/F010346/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council
    • Accession Number:
      0 (Proteins)
    • Publication Date:
      Date Created: 20210616 Date Completed: 20211109 Latest Revision: 20231107
    • Publication Date:
      20231215
    • Accession Number:
      PMC8328188
    • Accession Number:
      10.1039/d0cs00558d
    • Accession Number:
      34132259