Mechano-chemical feedback mediated competition for BMP signalling leads to pattern formation.

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  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: United States NLM ID: 0372762 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-564X (Electronic) Linking ISSN: 00121606 NLM ISO Abbreviation: Dev Biol Subsets: MEDLINE
    • Publication Information:
      Publication: San Diego, CA : Elsevier
      Original Publication: New York.
    • Subject Terms:
      Body Patterning* ; Gene Expression Regulation, Developmental* ; Signal Transduction*; Bone Morphogenetic Proteins/*metabolism ; Drosophila Proteins/*metabolism; Animals ; Bone Morphogenetic Proteins/genetics ; Drosophila Proteins/genetics ; Drosophila melanogaster ; Pupa ; Wings, Animal
    • Abstract:
      Developmental patterning is thought to be regulated by conserved signalling pathways. Initial patterns are often broad before refining to only those cells that commit to a particular fate. However, the mechanisms by which pattern refinement takes place remain to be addressed. Using the posterior crossvein (PCV) of the Drosophila pupal wing as a model, into which bone morphogenetic protein (BMP) ligand is extracellularly transported to instruct vein patterning, we investigate how pattern refinement is regulated. We found that BMP signalling induces apical enrichment of Myosin II in developing crossvein cells to regulate apical constriction. Live imaging of cellular behaviour indicates that changes in cell shape are dynamic and transient, only being maintained in those cells that retain vein fate competence after refinement. Disrupting cell shape changes throughout the PCV inhibits pattern refinement. In contrast, disrupting cell shape in only a subset of vein cells can result in a loss of BMP signalling. We propose that mechano-chemical feedback leads to competition for the developmental signal which plays a critical role in pattern refinement.
      Competing Interests: Declaration of competing interest The authors declare no conflict of interest.
      (Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.)
    • Contributed Indexing:
      Keywords: BMP signalling; Cell competition; Drosophila melanogaster; apical constriction; epithelial cells
    • Accession Number:
      0 (Bone Morphogenetic Proteins)
      0 (Drosophila Proteins)
    • Publication Date:
      Date Created: 20210923 Date Completed: 20211221 Latest Revision: 20211221
    • Publication Date:
      20231215
    • Accession Number:
      10.1016/j.ydbio.2021.09.006
    • Accession Number:
      34555363