Growth Differentiation Factor 7 promotes multiple-lineage differentiation in tenogenic cultures of mesenchymal stem cells.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0226040 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0267 (Electronic) Linking ISSN: 00201383 NLM ISO Abbreviation: Injury Subsets: MEDLINE
    • Publication Information:
      Publication: <2002->: Amsterdam : Elsevier
      Original Publication: Bristol, Wright.
    • Subject Terms:
      Bone Morphogenetic Proteins*/physiology ; Cell Differentiation* ; Mesenchymal Stem Cells*; Collagen/metabolism ; Animals ; Mice ; Cell Line ; Tendons ; Regeneration
    • Abstract:
      The repair of the tendon-bone interface, which is composed of tendon, fibrocartilage, and bony attachment, remains a clinical challenge. The application of mesenchymal stem cells (MSCs), collagen-rich extracellular matrix (ECMs), as well as growth factors, has the potential to regenerate this special multiple-tissue structure through the so-called biological augmentation. We present here an in vitro tendon regeneration model with C3H10T1/2 cells cultured on Collagen I matrix and evaluated the lineage determination effects of Growth Differentiation Factor 7 (GDF-7). We found that besides tenogenic effect, GDF-7 also stimulates the expression of osteoblastic as well as adipocytic genes. Our results indicate that GDF-7 might be a promising growth factor for regeneration of the tendon-bone interface due to its multiple-lineage stimulating effects. However, the side effect on adipogenic differentiation should be of concern, as it is a known risk factor for repair failures.
      Competing Interests: Declaration of interest None.
      (Copyright © 2022. Published by Elsevier Ltd.)
    • Contributed Indexing:
      Keywords: Adipocyte; Growth Differentiation Factor 7 (GDF-7); Mesenchymal stem cells (MSCs); Osteoblast; Tendon regeneration; Tenocyte
    • Accession Number:
      0 (Bone Morphogenetic Proteins)
      9007-34-5 (Collagen)
      0 (growth differentiation factor 7)
    • Publication Date:
      Date Created: 20221019 Date Completed: 20221129 Latest Revision: 20221129
    • Publication Date:
      20231215
    • Accession Number:
      10.1016/j.injury.2022.09.017
    • Accession Number:
      36261312