Isolation and Differentiation of Neurons and Glial Cells from Olfactory Epithelium in Living Subjects.

Publisher: Humana Press Country of Publication: United States NLM ID: 8900963 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1559-1182 (Electronic) Linking ISSN: 08937648 NLM ISO Abbreviation: Mol Neurobiol Subsets: MEDLINE

Original Publication: Clifton, NJ : Humana Press, c1987-

Cell Culture Techniques*/methods ; Cell Differentiation* ; Cell Separation*/methods ; Neurogenesis* ; Neuroglia*/cytology ; Neurons*/cytology ; Olfactory Mucosa*/cytology; Humans ; Basement Membrane/cytology ; Biomarkers/analysis ; Cell Adhesion ; Cell Proliferation ; Cells, Cultured ; Culture Media/chemistry ; Flow Cytometry ; Immunohistochemistry ; Magnetics ; Neural Stem Cells/cytology ; Organ Specificity

The study of psychiatric and neurological diseases requires the substrate in which the disorders occur, that is, the nervous tissue. Currently, several types of human bio-specimens are being used for research, including postmortem brains, cerebrospinal fluid, induced pluripotent stem (iPS) cells, and induced neuronal (iN) cells. However, these samples are far from providing a useful predictive, diagnostic, or prognostic biomarker. The olfactory epithelium is a region close to the brain that has received increased interest as a research tool for the study of brain mechanisms in complex neuropsychiatric and neurological diseases. The olfactory sensory neurons are replaced by neurogenesis throughout adult life from stem cells on the basement membrane. These stem cells are multipotent and can be propagated in neurospheres, proliferated in vitro and differentiated into multiple cell types including neurons and glia. For all these reasons, olfactory epithelium provides a unique resource for investigating neuronal molecular markers of neuropsychiatric and neurological diseases. Here, we describe the isolation and culture of human differentiated neurons and glial cells from olfactory epithelium of living subjects by an easy and non-invasive exfoliation method that may serve as a useful tool for the research in brain diseases.
(© 2023. The Author(s).)

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PID2019-106404RB-I00 Ministerio de Ciencia e Innovación; PNSD 2019I021 Plan Nacional sobre Drogas; 2019111082 Eusko Jaurlaritza; IT1211/19 Eusko Jaurlaritza; IT1512/22 Eusko Jaurlaritza

Keywords: Glia; Neurons; Neuropsychiatric diseases; Neurospheres; Olfactory epithelium; PSA-NCAM

0 (Biomarkers)
0 (Culture Media)

Date Created: 20230428 Date Completed: 20240214 Latest Revision: 20240406

20240406

PMC10293402

10.1007/s12035-023-03363-2

37118325